Abstract: Objective To search a good way to store CIK cells.Method Freeze CIK cells when they were induced for 14 days and anabiosis them after two months later.The anabiosis cells growth curve was drawn from the first day they recovered and the unfreezing cells growth curve was drawn after they induced for 14 days.The cytotoxic activities were determined by MTT and the cell phenotypes were detected by flow cytometry after anabiosis three days.Three indexes of anabiosis CIK cells were compared to the unfreezed.Results The anabiotic cells were in good condition.Compared with unfreezing CIK cells, the growth rate of freezed cells was lower, significant differences existed between the two groups(P＜0.05).Cytotoxic activities of CIK cells and the freezed ones were(51.1±2.0)%, (50.9±3.3)%, (36.4±3.2)% and(49.7±1.5)%, (34.7±1.3)%, (29.5±7.6)% according to the different effect and target cell ratios of 40∶1, 20∶1 and 10∶1.No significant difference existed between the two groups.The cell phenotypes CD3+CD56+ were(19.1±0.4)%, (19.5±0.8)%and there were no significant difference between the two groups.Conclusion Freezing had effect on the growth rate of CIK cells but had no influence on cytotoxic activity and cell phenotype.The method is available for clinical application.