RNA干扰抑制血管内皮生长因子影响舌癌耐药细胞移植瘤增殖及血管生成△

doi:10.3969/j.issn.1002-3070.2011.02.001

实用肿瘤学杂志 ›› 2011, Vol. 25 ›› Issue (2): 101-105.doi: 10.3969/j.issn.1002-3070.2011.02.001

• 论著 • 下一篇

RNA干扰抑制血管内皮生长因子影响舌癌耐药细胞移植瘤增殖及血管生成^△

李昊¹, 农晓琳¹, 黎彦¹, 夏勇¹, 李佳荃², 杨亦萍¹

1.广西医科大学口腔医学院(南宁 530021);
2.广西医科大学医学科学实验中心

收稿日期:2010-12-20 出版日期:2011-02-25 发布日期:2012-02-21
通讯作者: 农晓琳,E-mail:labnong@hotmail.com
作者简介:李昊,女,(1984-),硕士研究生,从事口腔颌面部肿瘤基础与临床治疗研究
基金资助:
国家自然科学基金项目(30060082);教育部“春晖计划”科研启动基金(教外司留[2003]593号);广西自然科学基金重点项目(2010GXNSFD013047);中国高等教育学会“十一五”教育科学研究规划立项课题(06AIL0770110);广西卫生厅重点科研项目(重200006,重200927);广西科学基金项目(桂科回0836013);广西教育厅硕士研究生科研创新项目(2007年重要课题16);广西研究生教育创新计划项目(2009105981003M174)

Inhibiting vascular endothelial growth factor by RNA interference affects proliferation and angiogenesis of drug-resistant tongue cancer cell xenografts

LI Hao¹, NONG Xiaolin¹, LI Yan¹, XIA Yong¹, LI Jiaquan², YANG Yiping¹

1.College of Stomatology,Guangxi Medical University,Nanning 530021;
2.Medical Science Research Center,Guangxi Medical University

Received:2010-12-20 Online:2011-02-25 Published:2012-02-21

摘要/Abstract

摘要：

目的研究RNA干扰抑制血管内皮生长因子(VEGF)的表达对舌癌耐药细胞移植瘤增殖及血管生成的影响。方法用顺铂体外连续诱导法诱导舌癌Tca8113细胞系获得耐药细胞,将耐药细胞接种裸鼠皮下建立移植瘤模型,并随机分为空白对照组、空载体组、无关片段组、干扰质粒组。以脂质体法对空载体组、无关片段组、干扰质粒组进行瘤内及瘤周注射转染,RNA干扰抑制VEGF的表达,测量瘤体重量及体积,免疫组化法检测移植瘤PCNA、CD34,比较肿瘤增殖性及微血管参数,原位杂交法检测VEGF mRNA,免疫印迹法检测VEGF蛋白。结果干扰质粒组的瘤体体积、重量、PCNA平均光密度值、微血管参数均小于其他各组(P＜0.05)。干扰质粒组中VEGF mRNA及蛋白表达水平较其他各组明显下降(P＜0.05)。结论通过靶向VEGF的RNA干扰下调VEGF的表达,能抑制人舌癌耐药细胞移植瘤的增殖及血管生成,提示RNA干扰抑制VEGF可能为临床治疗耐药舌癌提供新途径。

关键词: RNA干扰, 血管内皮生长因子, 舌癌, 耐药, 细胞系

Abstract:

Objective To investigate the effect of inhibiting vascular endothelial growth factor(VEGF)by RNA interference on proliferation and angiogenesis of drug-resistant tongue cancer cell xenografts.Methods Tca8113 cells were successively induced in vitro by cisplatin to acquire drug-resistance,and then injected subcutaneously in nude mice to establish xenograft models,which were randomly divided into non-transfected group,mock control group,scrambled sequence group and interference group.The transfection mediated by liposome was done in the latter 3 groups with intratumoral and peritumoral injection for inhibiting VEGF expression.Weight and size of xenografts were measured;immunohistochemical staining was applied to detect PCNA and CD34 for investigating tumor proliferation and microvessel parameters.In situ hybridization and Western blotting were used to test mRNA and protein of VEGF,respectively.Results The size,weight,mean optical density of PCNA protein and microvessel parameters of xenografts in interference group were less than those in other groups(P＜0.05).The expression of VEGF mRNA and protein in interference group decreased significantly,compared by the other 3 groups(P＜0.05).Conclusion RNA interference targeting VEGF could reduce proliferation and angiogenesis of drug-resistant tongue cancer cell xenografts through inhibiting VEGF expression,suggesting that inhibition of VEGF by RNA interference may serve as a new therapy for drug-resistant tongue cancer.

Key words: RNA interference, Vascular endothelial growth factor, Tongue cancer, Drug-resistance, Cell line

中图分类号:

R392.12

李昊, 农晓琳, 黎彦, 夏勇, 李佳荃, 杨亦萍. RNA干扰抑制血管内皮生长因子影响舌癌耐药细胞移植瘤增殖及血管生成^△[J]. 实用肿瘤学杂志, 2011, 25(2): 101-105.

LI Hao, NONG Xiaolin, LI Yan, XIA Yong, LI Jiaquan, YANG Yiping. Inhibiting vascular endothelial growth factor by RNA interference affects proliferation and angiogenesis of drug-resistant tongue cancer cell xenografts[J]. PRACTICAL ONCOLOGY JOURNAL, 2011, 25(2): 101-105.

[1]	罗干, 易超, 依马木买买提江·阿布拉, 徐林, 尹继炜, 丁伟. Notch信号通路在胰腺癌中的表达及其作用研究[J]. 实用肿瘤学杂志, 2019, 33(1): 1-7.
[2]	靳俊杰, 安静, 曹涤非, 宋爱利, 赵丽丽, 刘兆良. 组蛋白赖氨酸转移酶KAT6B基因shRNA慢病毒载体的构建与鉴定[J]. 实用肿瘤学杂志, 2018, 32(1): 1-6.
[3]	孙丽娜, 赵晓迪综述, 卢瑗瑗, 王新审校. LncRNA与胃肠道肿瘤化疗耐药的研究进展[J]. 实用肿瘤学杂志, 2017, 31(5): 448-453.
[4]	代欣综述, 张大昕审校. 非小细胞肺癌抗血管生成治疗研究现状[J]. 实用肿瘤学杂志, 2017, 31(5): 472-476.
[5]	曾玉兰,刘洋洋,梁金燕,谷飞飞,刘莉. 免疫检查点抑制剂耐药机制及耐药后治疗策略的研究进展[J]. 实用肿瘤学杂志, 2017, 31(4): 353-358.
[6]	黄剑，庞慧，宋雨航综述，蔡莉审校. microRNA在乳腺癌耐药中的作用[J]. 实用肿瘤学杂志, 2017, 31(3): 272-276.
[7]	于飞, 胡晶(综述), 陈雪松(审校). 乳腺癌他莫昔芬耐药机制的研究进展[J]. 实用肿瘤学杂志, 2017, 31(2): 160-164.
[8]	段佩雯, 赵苗苗综述, 王松坡审校. NF-κB对大肠癌发生发展的影响及其相关治疗的研究现状[J]. 实用肿瘤学杂志, 2017, 31(1): 43-47.
[9]	彭文苗，张志敏，胡萌，余丽芳，章必成，饶智国，秦传蓉. RPL23-siRNA干扰片段的构建及筛选[J]. 实用肿瘤学杂志, 2016, 30(6): 487-491.
[10]	赵郁，朱晓婷，王轶楠. ω-3多不饱和脂肪酸诱导多发性骨髓瘤细胞凋亡及抗增殖机制的研究[J]. 实用肿瘤学杂志, 2016, 30(6): 516-522.
[11]	张锐，王智煜，王帅，赵晖. 重组人血管内皮抑素联合NP方案治疗初诊晚期非小细胞肺癌骨转移疗效及对血清VEGF表达的影响[J]. 实用肿瘤学杂志, 2016, 30(6): 527-522.
[12]	任婷婷，邢影，综述，孟庆威，审校. 细胞自噬与肿瘤关系的研究进展[J]. 实用肿瘤学杂志, 2016, 30(6): 543-546.
[13]	张雪,王丽,燕相伟,许庆勇. Decorin基因表达对人肺腺癌A549细胞生物学行为的影响[J]. 实用肿瘤学杂志, 2016, 30(4): 289-294.
[14]	王静, 封安强, 张南征. 天然14肽生长抑素联合5-Fu对胃癌移植瘤的抑制作用[J]. 实用肿瘤学杂志, 2016, 30(3): 214-219.
[15]	陈龙综述, 钱诚审校. 血管内皮生长因子基因多态性与乳腺癌关系的研究进展[J]. 实用肿瘤学杂志, 2016, 30(3): 284-286.

RNA干扰抑制血管内皮生长因子影响舌癌耐药细胞移植瘤增殖及血管生成^△

Inhibiting vascular endothelial growth factor by RNA interference affects proliferation and angiogenesis of drug-resistant tongue cancer cell xenografts

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价