Abstract: Objective To explore effects of norcantharidin(NCTD)on the proliferation and killing activity of cytokine-induced killer cells(CIK).Methods Cord blood mononuclear cells were extracted by Ficoll density gradient method and then induced into CIK cells.The experiment was divided into NCTD-CIK group with a final concentration of 30μmol / L NCTD and control group CIK without NCTD.Changes in the proliferation of CIK cells,the immune phenotype and cytotoxic activity were observed using cell counting,flow cytometry and MTT method.Results The rate of cell proliferation and anti-tumor activity in NCTD-CIK group were significantly higher than those of the control CIK group,which showed statistical significance(P＜0.05).Conclusion NCTD promoted the expansion of CIK cells and enhanced their anti-tumor activity,which will provide a new approach for adoptive immunotherapy of CIK.