WEE1在胃癌组织中的表达及对患者预后的影响

doi:10.11904/j.issn.1002-3070.2019.03.009

实用肿瘤学杂志 ›› 2019, Vol. 33 ›› Issue (3): 239-243.doi: 10.11904/j.issn.1002-3070.2019.03.009

WEE1在胃癌组织中的表达及对患者预后的影响

孟海¹,杜观祥¹,朱利玲¹,汤琪云²

1.滨海县人民医院消化科(盐城 224500);
2.江苏省人民医院老年消化科

收稿日期:2018-12-11 修回日期:2019-01-04 出版日期:2019-06-20 发布日期:2019-06-18
通讯作者: 汤琪云,E-mail:zhujia6606@163.com
作者简介:孟海,男,(1970-),硕士,副主任医师,从事消化道肿瘤诊治的研究
基金资助:
盐城市科技计划项目(编号:YK2018080)

The expression of WEE1 in gastric cancer and its influence on prognosis of patients

MENG Hai¹,DU Guanxiang¹,ZHU Liling¹,TANG Qiyun²

1.Department of Gastroenterology,Binhai People′s Hospital,Yancheng 224500,China;
2.Department of Geriatric Gastroenterology,Jiangsu People′s Hospital

Received:2018-12-11 Revised:2019-01-04 Online:2019-06-20 Published:2019-06-18

摘要/Abstract

摘要： 目的探讨WEE1基因在胃癌组织中的表达及对患者预后的影响。方法收集78例胃癌患者为研究对象,根据有无淋巴结转移,将患者分为淋巴结(-)组与淋巴结(+)组,采用免疫组化及RT-qRCR技术检测胃癌组织中WEE1的表达,统计分析WEE1表达对胃癌淋巴结转移及预后的影响。结果 (1)WEE1在胃癌组织中的阳性率为43.6%,其中,低表达13例,高表达21例。淋巴结(+)组与淋巴结(-)组的WEE1的阳性率分别为53.8%(28/52)和23.1%(6/26);(2)RT-qPCR检测结果显示,淋巴结(-)组与淋巴结(+)组患者的平均WEE1 mRNA表达量分别为(1.32±0.21)、(3.64±0.41),差异具有统计学意义(P＜0.01);(3)ROC曲线结果显示,WEE1 mRNA表达水平对胃癌淋巴结转移预测的曲线下面积为0.806,敏感性84.8%,特异性79.6%,有较好的诊断效能。T分期、有无淋巴结转移是影响WEE1表达的独立性危险因素(P＜0.05);(4)78例患者,失访7例,失访率为8.9%,5年内死亡41例,其中,WEE1(-)组、WEE1低表达组、WEE1高表达组分别死亡13例、10例、18例,三组患者的5年生存率有统计学差异(χ²=25.67,P＜0.001)。两两比较后发现,WEE1低表达组与WEE1高表达的生存率无统计学差异(P＞0.05),但均显著低于WEE1(-)组(P＜0.05)。结论 WEE1在胃癌中有较高的阳性率,且与患者分期及淋巴结转移密切相关,其阳性表达是患者预后不良的强烈信号。

关键词: 胃癌, WEE1, 淋巴结转移

Abstract: Objective The aim of this study was to investigate the expression of WEE1 in gastric cancer and its influence on prognosis of patients.Methods Seventy-eight patients with gastric cancer were enrolled.According to the presence or absence of lymph node(LN)metastasis,the patients were divided into LN(-)group and LN(+)group.The expression of WEE1 in gastric cancer tissues was detected by immunohistochemistry and RT-qRCR techniques.The influence of WEE1 expression on LN metastasis and prognosis of gastric cancer were analyzed statistically.Results (1)The positive rate of WEE1 in gastric cancer was 43.6%,of which 13 cases were a low expression and 21 cases with high expression.The positive rates of WEE1 in LN(+)group and LN(-)group were 53.8% and 23.1%,respectively.(2)The results of RT-qPCR showed that the mRNA expression of WEE1 in LN(-)and LN(+)groups was(1.32±0.21)and(3.64±0.41),respectively,and the difference was statistically significant(P＜0.01).(3)The results of ROC curve showed that the area under curve(AUC)of WEE1 mRNA expression level for LN metastasis of gastric cancer was 0.806,the sensitivity was 84.8%,the specificity was 79.6%,and the diagnostic efficacy was good.T stage and LN metastasis were independent risk factors for the expression of WEE1(P＜0.05).(4)Among 78 patients,7 patients were lost to follow-up,the rate of loss of follow-up was 8.9%,and 41 patients died within 5 years.Among them,13,10 and 18 died in the WEE1(-)group,low-expression of WEE1 group and high-expression of WEE1 group,respectively.The 5-year survival rates of the three groups were significantly different(χ²=25.67,P＜0.001).The survival rate in the low expression of WEE1 group was not significantly different from the high expression of WEE1 group(P＞0.05),but both of them were significantly low the WEE1(-)group(P＜0.05).Conclusion WEE1 has a high positive rate in gastric cancer,and is closely related to patient stage and LN metastasis.The positive expression of WEE1 is a strong signal for poor prognosis.

Key words: Gastric cancer, WEE1, Lymph node metastasis

中图分类号:

R735.2

孟海,杜观祥,朱利玲,汤琪云. WEE1在胃癌组织中的表达及对患者预后的影响[J]. 实用肿瘤学杂志, 2019, 33(3): 239-243.

MENG Hai,DU Guanxiang,ZHU Liling,TANG Qiyun. The expression of WEE1 in gastric cancer and its influence on prognosis of patients[J]. PRACTICAL ONCOLOGY JOURNAL, 2019, 33(3): 239-243.

参考文献 1

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WEE1在胃癌组织中的表达及对患者预后的影响

The expression of WEE1 in gastric cancer and its influence on prognosis of patients

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