miRNA-409-3p表达水平对宫颈癌细胞增殖及顺铂化疗敏感性的影响

doi:10.11904/j.issn.1002-3070.2019.04.003

摘要/Abstract

摘要： 目的探讨微小RNA-409-3p(miRNA-409-3p)表达水平对宫颈癌细胞增殖及顺铂化疗敏感性的影响。方法将HeLa细胞分为正常对照组、NC对照组和miRNA-409-3p mimic组,RT-PCR法检测不同宫颈组织及转染miRNA-409-3p mimic后各组细胞中miRNA-409-3p mRNA的表达,CCK-8法检测细胞增殖率,Western blot法检测Fip200、LC3、Caspase-3蛋白的表达。结果宫颈癌组织中miRNA-409-3p mRNA的相对表达水平显著低于正常宫颈组织和癌旁组织(P＜0.05);通过转染miRNA-409-3p mimic后,miRNA-409-3p mimic组miRNA-409-3p mRNA的相对表达水平较正常对照组显著上调(P＜0.05),NC对照组miRNA-409-3p mRNA的相对表达水平较正常对照组无统计学差异(P＞0.05)。miRNA-409-3p mimic组HeLa细胞增殖率较正常对照组和NC对照组显著降低(P＜0.05)。给予顺铂干预后,miRNA-409-3p mimic组细胞增殖显著被抑制(P＜0.05);同时,miRNA-409-3p mimic组细胞中Fip200蛋白表达水平和LC3Ⅱ/LC3Ⅰ比值显著低于正常对照组和NC对照组(P＜0.05)。结论 miRNA-409-3p在宫颈癌组织中低表达,可能与宫颈癌的发生发展有关,上调miRNA-409-3p水平可以抑制HeLa细胞增殖,增加HeLa细胞对顺铂的敏感性。

关键词: miRNA-409-3p, 宫颈癌, 细胞增殖, 顺铂敏感性

Abstract: Objective The Objective of this study was to investigate the effect of microRNA-409-3p(miRNA-409-3p)expression on proliferation of cervical cancer Hela cells and chemotherapy sensitivity of cisplatin.Methods HeLa cells were divided into normal control,miRNA-409-3p mimic and RNA control groups.The mRNA expression of miRNA-409-3p was detected by RT-qPCR.The cell viability was detected by CCK-8 assay,and the expression of Fip200,LC3 and Caspase-3 was detected by Western blot.Results The relative expression level of microRNA-409-3p mRNA in cervical cancer tissues was significantly lower than that in normal cervical tissues and adjacent tissues(P＜0.05).After transfection of microRNA-409-3p mimic,the relative expression level of microRNA-409-3p mRNA in the microNA-409-3p mimic group was significantly up-regulated compared with the normal control group(P＜0.05).The relative expression level of miRNA-409-3p mRNA in the RNA control group was not different from that in the normal control group(P＞0.05).The proliferative rate of HeLa cells int the microRNA-409 mimic group was significantly lower than that in normal control and RNA control groups(P＜0.05).After cisplatin treatment,the proliferation of Hela cells was significantly inhibited in the miRNA-409-3p mimic group(P＜0.05);the expression of Fip200 protein and LC3II/LC3I ratio in the microRNA-409-3p mimic group were significantly lower than those in the normal control and RNA control groups(P＜0.05).Conclusion The low expression of microRNA-409-3p in cervical cancer tissue may be related to the occurrence and development of cervical cancer.Upregulation of microRNA-409-3p level can inhibit the proliferation of HeLa cells and increase the sensitivity of HeLa cells to cisplatin.

Key words: miRNA-409-3p, Cervical cancer, Cell proliferation, Cisplatin sensitivity

中图分类号:

R737.33

申利, 魏军成. miRNA-409-3p表达水平对宫颈癌细胞增殖及顺铂化疗敏感性的影响[J]. 实用肿瘤学杂志, 2019, 33(4): 300-304.

SHEN Li, WEI Juncheng. Effect of miRNA-409-3p expression on proliferation of cervical cancer cells and chemotherapy sensitivity of cisplatin[J]. Journal of Practical Oncology, 2019, 33(4): 300-304.

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