ZCCHC12对甲状腺细胞恶性转化的影响与作用机制

doi:10.11904/j.issn.1002-3070.2020.02.009

实用肿瘤学杂志 ›› 2020, Vol. 34 ›› Issue (2): 133-138.doi: 10.11904/j.issn.1002-3070.2020.02.009

ZCCHC12对甲状腺细胞恶性转化的影响与作用机制

刘光霞¹, 赵连春², 陈芳¹, 贾乾³, 高伟⁴, 侯瞻¹, 卢亚敏¹

1.河北省人民医院核医学科(石家庄 050051);
2.河北以岭医院检验科;
3.河北省人民医院体检中心;
4.河北省人民医院检验科

收稿日期:2019-10-27 发布日期:2020-04-27
通讯作者: 卢亚敏,E-mail:yamin_yml@163.com
作者简介:刘光霞,女,(1984-),硕士,主管技师,从事核医学检验、癌症方面的研究。
基金资助:
青年科技课题计划(编号:20190232)

Effect and mechanism of ZCCHC12 on malignant transformation of thyroid cells

LIU Guangxia¹, ZHAO Lianchun², CHEN Fang¹, JIA Qian³, GAO Wei⁴, HOU Zhan¹, LU Yamin¹

1.Department of Nuclear Medicine,Hebei General Hospital,Shijiazhuang 050051,China;
2.Department of Clinical Laboratory,Hebei Yiling Hospita;
3.Physical Examination Center,Hebei General Hospital;
4.Department of Clinical Laboratory,Hebei General Hospital

Received:2019-10-27 Published:2020-04-27

摘要/Abstract

摘要： 目的分析含CCHC结构域的锌指蛋白12(ZCCHC12)在甲状腺癌组织与甲状腺癌细胞系中的表达,并探讨其对甲状腺细胞恶性转化的影响与机制。方法采用RT-PCR与Western blot检测30例甲状腺癌患者肿瘤组织和10例良性甲状腺组织以及甲状腺癌细胞系中ZCCHC12的表达。以ZCCHC12腺病毒载体或siRNA转染法处理甲状腺癌细胞,检测细胞增殖、侵袭能力以及相应分子的表达变化情况。结果与良性甲状腺组织相比,甲状腺癌肿瘤组织中ZCCHC12的mRNA和蛋白表达水平均升高(P＜0.05);同时与甲状腺滤泡上皮细胞HUM-CELL-0097相比,甲状腺癌细胞中ZCCHC12的MRNA和蛋白表达也明显升高(P＜0.05)。ZCCHC12过表达后甲状腺癌细胞增殖与侵袭能力显著上升(P＜0.05),相反,ZCCHC12沉默后其增殖和侵袭能力受到抑制(P＜0.05)。与对照组相比,ZCCHC12过表达后细胞周期蛋白D(cyclin D)及基质金属蛋白酶2(MMP-2)、基质金属蛋白酶9(MMP-9)的表达水平增加;ZCCHC12沉默后则降低(P＜0.05)。结论 ZCCHC12在甲状腺癌组织及细胞中表达升高,其过表达后甲状腺癌细胞增殖活力与侵袭能力上升。ZCCHC12异常表达可激活细胞周期相关蛋白及胞外基质相关酶的表达,加剧甲状腺细胞的恶性转化。

关键词: ZCCHC12, 甲状腺癌, 增殖, 侵袭, 分子机制

Abstract: Objective The aims of this study were to analyze the expression of zinc finger protein 12(ZCCHC12)containing CCHC domain in thyroid cancer tissues and cells,and to explore its effect and mechanism on malignant transformation of thyroid cells.Methods The expression of ZCCHC12 in tumor tissues from 30 thyroid cancer patients,10 benign thyroid tissues and cancer cell lines were detected by qRT-PCR and Western blot.ZCCHC12 adenovirus vector or siRNA was used to transfect thyroid cancer cells,and the cell proliferation,invasion ability and expression of corresponding molecules were detected.Results Compared with benign thyroid tissues,the expression of ZCCHC12 in thyroid cancer tissues increased(P＜0.05).At the same time,compared with thyroid follicular epithelial HUM-CELL-0097 cells,the expression of ZCCHC12 at levels of mRNA and protein in thyroid cancer cells also significantly increased(P＜0.05).After ZCCHC12 overexpression,the proliferation and invasive ability of thyroid cancer cells increased significantly(P＜0.05).On the contrary,the proliferation and invasion ability of ZCCHC12 was inhibited after ZCCHC12 silencing(P＜0.05).Compared with the control group,the expression of cyclin D,matrix metalloproteinase 2(MMP-2)and MMP-9 increased after ZCCHC12 overexpression and their expression decreased after ZCCHC12 silencing(P＜0.05).Conclusion ZCCHC12 is highly expressed in thyroid cancer tissues and thyroid cancer cells,and its proliferative activity and invasion ability increase after overexpression ZCCHC12.The abnormal expression of ZCCHC12 can activate the expression of cell cycle related proteins and extracellular matrix related enzymes,and aggravate the malignant transformation of thyroid cells.

Key words: ZCCHC12, Thyroid cancer, Proliferation, Invasive, Molecular mechanism

中图分类号:

R730.23

刘光霞, 赵连春, 陈芳, 贾乾, 高伟, 侯瞻, 卢亚敏. ZCCHC12对甲状腺细胞恶性转化的影响与作用机制[J]. 实用肿瘤学杂志, 2020, 34(2): 133-138.

LIU Guangxia, ZHAO Lianchun, CHEN Fang, JIA Qian, GAO Wei, HOU Zhan, LU Yamin. Effect and mechanism of ZCCHC12 on malignant transformation of thyroid cells[J]. Journal of Practical Oncology, 2020, 34(2): 133-138.

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http://journal09.magtechjournal.com/Jwk3_syzlxzz/CN/Y2020/V34/I2/133

参考文献

1 高明,郑向前.甲状腺癌过去与未来十年[J].中国肿瘤临床,2018,45(1):2-6.
2 Roman BR,Morris LG,Davies L.The thyroid cancer epidemic,2017 perspective[J].Curr Opin Endocrinol Diabetes Obes,2017,24(5):332-336.
3 Wachter S,Damanakis AI,Elxnat M,et al.Epigenetic modifications in thyroid cancer cells restore NIS and radio-iodine uptake and promote cell death[J].J Clin Med,2018,7(4):61.
4 Ginam C,Bhat SS,Jinsong G,et al.Evidence that SIZN1 is a candidate X-linked mental retardation gene[J].Am J Med Genet Part A,2010,146(20):2644-2650.
5 杨辉,牛美芝,郭晓丽,等.重组腺病毒介导MRTF-A基因干预缺血再灌注损伤模型大鼠心肌细胞的凋亡[J].中国组织工程研究,2018,22(24):77-82.
6 Wang J,Chi S,Huang Z,et al.Comprehensive characterization of differentially expressed genes in thyroid cancer[J].Future Oncol,2017,13(24):2159.
7 Xia S,Ji R,Xu Y,et al.Twisted gastrulation BMP signaling modulator 1 regulates papillary thyroid cancer cell motility and proliferation[J].J Cancer,2017,8(14):2816-2827.
8 Zhao JJ,Hao S,Wang LL,et al.Long non-coding RNA ANRIL promotes the invasion and metastasis of thyroid cancer cells through TGF-β/Smad signaling pathway[J].Oncotarget,2016,7(36):57903-57918.
9 董宇,申亚伟,马建仓,等.TGF-β1和BMP-2在分化型甲状腺癌中的表达和意义[J].西安交通大学学报(医学版),2012,33(6):765-768.
10 刘楠楠,于春艳,王丹,等.Smad4蛋白在良恶性甲状腺病变组织中的表达及意义[J].中国老年学,2017,37(5):1077-1078.
11 Yan C,Su H,Song X,et al.Smad ubiquitination regulatory factor 1(Smurf1)promotes thyroid cancer cell proliferation and migration via ubiquitin-dependent degradation of Kisspeptin-1[J].Cell Physiol Biochem,2018,49(5):2047-2059.
12 Bhatti MZ,Pan L,Wang T,et al.REGγ potentiates TGF-β/Smad signal dependent epithelial-mesenchymal transition in thyroid cancer cells[J].Cell Signal,2019,64:109412.
13 Ginam C,Youngshin L,Dina Z,et al.Sizn1 is a novel protein that functions as a transcriptional coactivator of bone morphogenic protein signaling[J].Mol Cell Biol,2008,28(5):1565-1572.
14 Li H,Liu Q,Hu X,et al.Human ZCCHC12 activates AP-1 and CREB signaling as a transcriptional co-activator[J].Acta Biochim Biophys Sin,2009,41(7):535-544.
15 Shingo I,Miho R,Mitsuyoshi H,et al.GLI1 interferes with the DNA mismatch repair system in pancreatic cancer through BHLHE41-mediated suppression of MLH1[J].Cancer Res,2013,73(24):7313-7323.
16 Qiu-Li L,Fu-Jin C,Renchun L,et al.ZCCHC12,a potential molecular marker of papillary thyroid carcinoma:a preliminary study[J].Med Oncol,2012,29(3):1409-1417.
17 Wang O,Zheng Z,Wang Q,et al.ZCCHC12,a novel oncogene in papillary thyroid cancer[J].J Cancer Res Clin Oncol,2017,143(9):1679-1686.

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ZCCHC12对甲状腺细胞恶性转化的影响与作用机制

Effect and mechanism of ZCCHC12 on malignant transformation of thyroid cells

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