丙泊酚经HIF-1α信号通路对头颈鳞状细胞癌SCC-74细胞增殖、凋亡与侵袭的影响

doi:10.11904/j.issn.1002-3070.2020.02.007

摘要/Abstract

摘要： 目的探讨丙泊酚经HIF-1α信号通路对头颈鳞状细胞癌SCC-74细胞增殖、凋亡与侵袭的影响。方法体外培养SCC-74细胞,丙泊酚低剂量组、丙泊酚中剂量组和丙泊酚高剂量组分别给予终浓度为2.5、10、20 μg/mL的丙泊酚,对照组给予等量DMEM培养液,继续培养48 h后收获细胞,检测SCC-74细胞增殖、凋亡与侵袭情况,同时检测SCC-74细胞中Akt、p-Akt、HIF-1α、Fas、FasL、Bcl-2、Bax蛋白和mRNA水平。结果与对照组比较,各丙泊酚剂量组SCC-74细胞增殖率和侵袭数降低,细胞凋亡率增加(P＜0.05);与对照组比较,各丙泊酚剂量组SCC-74细胞中p-Akt蛋白、Bcl-2蛋白和mRNA水平降低,HIF-1α、Fas、FasL和Bax蛋白和mRNA水平增高,呈剂量依赖性(P＜0.05)。各组SCC-74细胞中Akt蛋白和mRNA水平无统计学差异(P＞0.05)。结论丙泊酚通过激活HIF-1α信号通路,抑制SCC-74细胞增殖和侵袭,促进SCC-74细胞凋亡。

关键词: 丙泊酚, HIF-1α信号通路, 头颈鳞状细胞癌, 细胞增殖, 细胞凋亡, 细胞侵袭

Abstract: Objective The aim of this study was to investigate,To investigate the effects of propofol on the proliferation,apoptosis and invasion of SCC-74 cells via HIF-1 α signal pathway.Methods SCC-74 cells were cultured in vitro.The final concentrations of propofol were 2.5,10 and 20 μg/mL in low-dose propofol groups,medium-dose propofol group and high-dose propofol groups,respectively.The control group was given the same amount of DMEM culture medium,and the cells were harvested after treatment for 48h.The proliferation,apoptosis and invasion of SCC-74 cells were detected.At the same time,the levels of Akt,p-Akt,HIF-1 α,Fas,FasL,Bcl-2 and Bax protein and mRNA were detected.Results Compared with the control group,the proliferation rate and invasion number of SCC-74 cells in all propofol dose groups was significantly decreased,the apoptosis rate of SCC-74 cells increased(P＜0.05),and the levels of p-Akt and bcl-2 protein in SCC-74 cells was significantly decreased,the levels of HIF-1 α,Bcl-2,Fas,FasL and Bax protein increased in a dose-dependent manner(P＜0.05).There was no significant change in the expression of Akt in SCC-74 cells(P＞0.05).Conclusion Propofol can inhibit the proliferation and invasion of SCC-74 cells and promote the apoptosis of SCC-74 cells by activating HIF-1 α signal pathway.

Key words: Propofol, HIF-1 α signal pathway, Squamous cell carcinoma of head and neck, Cell proliferation, Apoptosis, Cell invasion

中图分类号:

R739.91

王飞, 朱贲贲. 丙泊酚经HIF-1α信号通路对头颈鳞状细胞癌SCC-74细胞增殖、凋亡与侵袭的影响[J]. 实用肿瘤学杂志, 2020, 34(2): 120-125.

WANG Fei, ZHU Benben. Effects of propofol on the proliferation,apoptosis and invasion of SCC-74 cells via HIF-1 α signal pathway[J]. Journal of Practical Oncology, 2020, 34(2): 120-125.

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链接本文: http://journal09.magtechjournal.com/Jwk3_syzlxzz/CN/10.11904/j.issn.1002-3070.2020.02.007

http://journal09.magtechjournal.com/Jwk3_syzlxzz/CN/Y2020/V34/I2/120

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