长链非编码RNA OTUD6B-AS1对肺腺癌A549细胞增殖、侵袭和迁移能力的影响

doi:10.11904/j.issn.1002-3070.2024.01.003

实用肿瘤学杂志 ›› 2024, Vol. 38 ›› Issue (1): 13-17.doi: 10.11904/j.issn.1002-3070.2024.01.003

长链非编码RNA OTUD6B-AS1对肺腺癌A549细胞增殖、侵袭和迁移能力的影响

薄芸^1,2, 杭文璐², 左淑润², 董圆², 李海泉²

1.安徽理工大学医学院(安徽 232001);
2.徐州医科大学第二附属医院呼吸与危重症医学科

收稿日期:2023-04-26 修回日期:2024-01-05 出版日期:2024-02-28 发布日期:2024-06-21
通讯作者: 李海泉,E-mail:8711860@qq.com
作者简介:薄芸,女,(1998-),硕士研究生,从事呼吸系统肿瘤诊治方向的研究。
基金资助:
徐州市科技局医药卫生面上项目(编号:KC22207,KC22212);江苏省自然科学基金青年项目(编号:BK20220236);安徽理工大学研究生创新基金项目(编号:2022CX2143)

Effects of long non-coding RNA OTUD6B-AS1 on proliferation,invasion and migration of lung adenocarcinoma A549 cells

BO Yun^1,2, HANG Wenlu², ZUO Shurun², Dong Yuan², LI Haiquan²

1. Medical College of Anhui University of Science and Technology,Anhui 232001,China;
2. Department of Respiratory and Critical Care Medicine,Second Affiliated Hospital of Xuzhou Medical University

Received:2023-04-26 Revised:2024-01-05 Online:2024-02-28 Published:2024-06-21

摘要/Abstract

摘要： 目的探讨lncRNA OTUD6B-AS1对肺腺癌A549细胞增殖、迁移和侵袭能力的影响。方法体外培养肺腺癌A549细胞系,瞬时转染OTUD6B-AS1和空载质粒构建过表达和对照细胞模型,分为OTUD6B-AS1过表达组和空载质粒组(NC组)。通过qRT-PCR实验验证OTUD6B-AS1的转染效率;采用CCK-8实验检测OTUD6B-AS1对肺腺癌细胞增殖活性的影响,采用Transwell法检测OTUD6B-AS1对肺腺癌细胞迁移和侵袭能力的影响。结果 qRT-PCR检测显示,与NC组相比,过表达组的OTUD6B-AS1表达量显著增高(P＜0.05);CCK-8细胞活性检测结果显示,OTUD6B-AS1过表达组的肺腺癌A549细胞增殖活性较NC组显著降低(P＜0.05);Transwell侵袭实验结果表明,OTUD6B-AS1过表达组细胞迁移和侵袭能力均显著低于NC组(P＜0.05)。结论在肺腺癌细胞A549中lncRNA OTUD6B-AS1的过表达可以抑制A549细胞增殖、迁移和侵袭的能力。

关键词: 长链非编码RNA, 卵巢肿瘤结构域的6B反义RNA1, 肺癌, 迁移, 侵袭

Abstract: Objective The aim of this study was to investigate the effects of LncRNA OTUD6B-AS1 on the proliferation,migration and invasion of lung adenocarcinoma A549 cells. Methods Lung adenocarcinoma A549 cell line was cultured in vitro,and transient transfection of OTUD6B-AS1 and empty plasmid group were used as the control group.Overexpression and control cell models were constructed,and divided into OTUD6B-AS1 overexpression group and empty plasmid group(NC group).The cell model was divided into the empty plasmid group(NC group)and OTUD6B-AS1 overexpression group.The transfection efficiency of OTUD6B-AS1 mRNA was verified through qRT-PCR.The CCK-8 experiment was used to detect the effect of OTUD6B-AS1 on the proliferation activity of lung adenocarcinoma cells,and the Transwell assay was used to detect the effect of OTUD6B-AS1 on the migration and invasion ability of lung adenocarcinoma cells. Results Compared to the NC group,the overexpression OTUD6B-AS1 group had a significant increase in the expression of OTUD6B-AS1(P＜0.05).The CCK-8 assay results showed that the proliferation activity of A549 cells in the OTUD6B-AS1 overexpression group was significantly reduced compared to the NC group(P＜0.05).The results of the Transwell assay showed that the OTUD6B-AS1 overexpression group had significantly lower cell migration and invasion abilities than the NC group(P＜0.05). Conclusion Overexpression lncRNA OTUD6B-AS1 in lung adenocarcinoma A549 cells can significantly inhibit the proliferation,migration,and invasion ability of A549 cells.

Key words: Long chain non coding RNA, Ovarian tumor domain containing 6B antisense RNA1, Lung cancer, Migration, Invasion

中图分类号:

R734.2

薄芸, 杭文璐, 左淑润, 董圆, 李海泉. 长链非编码RNA OTUD6B-AS1对肺腺癌A549细胞增殖、侵袭和迁移能力的影响[J]. 实用肿瘤学杂志, 2024, 38(1): 13-17.

BO Yun, HANG Wenlu, ZUO Shurun, Dong Yuan, LI Haiquan. Effects of long non-coding RNA OTUD6B-AS1 on proliferation,invasion and migration of lung adenocarcinoma A549 cells[J]. Journal of Practical Oncology, 2024, 38(1): 13-17.

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长链非编码RNA OTUD6B-AS1对肺腺癌A549细胞增殖、侵袭和迁移能力的影响

Effects of long non-coding RNA OTUD6B-AS1 on proliferation,invasion and migration of lung adenocarcinoma A549 cells

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