miR-765靶向ARID1A对结直肠癌细胞侵袭及迁移的影响

doi:10.11904/j.issn.1002-3070.2023.01.006

实用肿瘤学杂志 ›› 2023, Vol. 37 ›› Issue (1): 32-38.doi: 10.11904/j.issn.1002-3070.2023.01.006

miR-765靶向ARID1A对结直肠癌细胞侵袭及迁移的影响

周俊峰¹, 曾之耀¹, 骆永富¹, 贺超²

1.永州市中心医院胃肠外科(永州 425000);
2.南华大学附属第一医院急诊科

收稿日期:2022-02-25 修回日期:2022-12-08 出版日期:2023-02-28 发布日期:2023-03-21
通讯作者: 周俊峰,E-mail:a15976158998@126.com
作者简介:周俊峰,男,(1988-),本科,主治医师,从事直肠癌的基础和临床研究。

Effects of miR-765 targeting ARID1A on the invasion and migration of colorectal cancer cells

ZHOU Junfeng¹, ZENG Zhiyao¹, LUO Yongfu¹, HE Chao²

1. Department of Gastrointestinal Surgery,Yongzhou Central Hospital,Yongzhou 425000,China;
2. Emergency Department,The First Affiliated Hospital of South China University

Received:2022-02-25 Revised:2022-12-08 Online:2023-02-28 Published:2023-03-21

摘要/Abstract

摘要： 目的探讨微小核糖核酸-765(miR-765)靶向AT丰富结合域蛋白1A(ARID1A)对结直肠癌细胞侵袭及迁移的影响。方法收集35例结直肠癌患者的结直肠癌组织及癌旁组织,体外培养结直肠癌SW480细胞并分为对照组、siRNA NC组、miR-765 siRNA组、mimic NC组和miR-765 mimic组。qRT-PCR法检测miR-765、ARID1A mRNA表达,Transwell法检测SW480细胞迁移和侵袭情况,蛋白印迹法检测ARID1A、基质金属蛋白酶-9(MMP-9)、血管内皮生长因子(VEGF)蛋白表达,双荧光素酶实验检测miR-765与ARID1A的靶向关系。结果与癌旁组织相比,结直肠癌组织中miR-765水平显著升高,ARID1A mRNA及蛋白水平显著降低(P＜0.001),且结直肠癌组织中miR-765与ARID1A mRNA呈显著负相关(r=-0.768,P＜0.001);与TNM分期为Ⅰ~Ⅱ期的患者相比,Ⅲ~Ⅳ期的患者结直肠癌组织中miR-765水平显著升高(P＜0.01),ARID1A蛋白水平显著降低(P＜0.001)。敲减miR-765表达后SW480细胞迁移数、侵袭数、miR-765水平及MMP-9、VEGF蛋白水平显著降低,ARID1A水平显著升高(P＜0.05);过表达miR-765后SW480细胞迁移数、侵袭数、miR-765水平及MMP-9、VEGF蛋白水平显著升高,ARID1A水平显著降低(P＜0.05)。miR-765靶向调控ARID1A表达。结论 miR-765可能通过靶向抑制ARID1A表达,促进结直肠癌SW480细胞的侵袭和迁移过程。

关键词: 微小核糖核酸-765, AT丰富结合域蛋白1A, 结直肠癌, 迁移, 侵袭

Abstract: Objective The aim of this study was to investigate the effect of microRNA-765(miR-765)targeting AT-rich interactive domain-containing protein 1A(ARID1A)on the invasion and migration of colorectal cancer cells. Methods Colorectal cancer tissues and adjacent tissues were collected from 35 patients with colorectal cancer,SW480 cells were cultured and divided into the control group,siRNA NC group,miR-765 siRNA group,mimic NC group,and miR-765 mimic group.qRT-PCR was used to detect the expression of miR-765 and ARID1A mRNA.Transwell assay was used to detect the migration and invasion of SW480 cells.Western blot was used to detect the expression of ARID1A,matrix metalloproteinase-9(MMP-9)and vascular endothelial growth factor(VEGF)protein.Dual luciferase assay was used to detect the targeting relationship between miR-765 and ARID1A. Results Compared with adjacent tissues,the level of miR-765 in colorectal cancer tissues was significantly increased,and the ARID1A at levels of mRNA and protein were significantly reduced(P＜0.001),and there was a significant negative correlation between miR-765 and ARID1A mRNA in colorectal cancer tissues(r=-0.768,P＜0.001).Compared with TNM stage Ⅰ-Ⅱ patients,the level of miR-765 in colorectal cancer tissues of patients with TNM stage III to IV was significantly increased(P＜0.01),and the expression of ARID1A protein was significantly decreased(P＜0.001).After knockdown -765 expression,the number of migration and invasion,miR-765 level,the levels of MMP-9 and VEGF protein were significantly reduced in SW480 cells,and the expression of ARID1A protein was significantly increased(P＜0.05).After overexpression of miR-765,the number migration and invasion,miR-765 level,the levels of MMP-9 and VEGF protein were significantly increased in SW480 cells,while the expression of ARID1A was significantly reduced(P＜0.05).miR-765 targeted and regulated ARID1A expression. Conclusion miR-765 can promote the invasion and migration of SW480 cells by targeting and inhibiting the expression of ARID1A.

Key words: microRNA-765, AT-rich interactive domain-containing protein 1A, Colorectal cancer, Migration, Invasion

中图分类号:

R735.3

周俊峰, 曾之耀, 骆永富, 贺超. miR-765靶向ARID1A对结直肠癌细胞侵袭及迁移的影响[J]. 实用肿瘤学杂志, 2023, 37(1): 32-38.

ZHOU Junfeng, ZENG Zhiyao, LUO Yongfu, HE Chao. Effects of miR-765 targeting ARID1A on the invasion and migration of colorectal cancer cells[J]. Journal of Practical Oncology, 2023, 37(1): 32-38.

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miR-765靶向ARID1A对结直肠癌细胞侵袭及迁移的影响

Effects of miR-765 targeting ARID1A on the invasion and migration of colorectal cancer cells

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