POM121在头颈部黏膜黑色素瘤中的表达及临床意义

doi:10.11904/j.issn.1002-3070.2025.02.006

实用肿瘤学杂志 ›› 2025, Vol. 39 ›› Issue (2): 108-115.doi: 10.11904/j.issn.1002-3070.2025.02.006

POM121在头颈部黏膜黑色素瘤中的表达及临床意义

张塨钰, 郭浩兵, 鲁莉莉, 宋宁扬, 朴松林

哈尔滨医科大学附属第一医院口腔颌面外科(哈尔滨 150001)

收稿日期:2024-10-20 修回日期:2025-01-24 出版日期:2025-04-28 发布日期:2025-05-06
通讯作者: 朴松林,E-mail:songlinpiao@126.com
作者简介:张塨钰,男,(1996—),硕士研究生,从事头颈部肿瘤相关的研究。
基金资助:
黑龙江省自然科学基金项目(编号:LH2022H045)

Expression and clinical significance of POM121 in head and neck mucosal melanoma

ZHANG Gongyu, GUO Haobing, LU Lili, SONG Ningyang, PIAO Songlin

Department of Oral and Maxillofacial Surgery,The First Affiliated Hospital of Harbin Medical University,Harbin 150001, China

Received:2024-10-20 Revised:2025-01-24 Online:2025-04-28 Published:2025-05-06

摘要/Abstract

摘要： 目的探讨核孔蛋白121(nuclear pore membrane protein 121,POM121)在头颈部黏膜黑色素瘤(head and neck mucosal melanoma,HNMM)中的表达情况及其对HNMM细胞侵袭和迁移的影响。方法收集2011年3月1日—2015年3月30日就诊于哈尔滨医科大学附属肿瘤医院的63例HNMM患者的癌组织及20例癌旁正常口腔上皮组织,采用免疫组织化学法检测POM121的表达水平。分析POM121表达水平与HNMM患者临床病理特征的关系及其对患者预后的影响。Western blot检测HNMM细胞(MM9H-1)和正常口腔上皮细胞(human oral keratinocytes,HOK)中POM121的表达。应用干扰小RNA(small interfering RNA,siRNA)敲低POM121后,通过划痕实验与Transwell实验检测其对HNMM细胞迁移与侵袭能力的影响。结果免疫组织化学法检测显示POM121在HNMM组织中表达水平显著高于癌旁组织(P＜0.001),POM121的表达与肿瘤大小和肿瘤分期有关(P＜0.05);Kaplan-Meier分析显示,POM121低表达患者的无病生存期(disease-free survival,DFS)与总生存期(overall survival,OS)高于POM121高表达患者;Cox回归分析显示,POM121的表达情况、AJCC分期及淋巴结转移为HNMM患者OS以及DFS的影响因素(P＜0.05);Western blot结果显示,POM121在MM9H-1细胞中的蛋白表达水平高于HOK细胞(P＜0.05);敲低POM121后,细胞迁移率和细胞侵袭能力均明显降低(P＜0.001)。结论 POM121在HNMM组织与细胞中高表达,下调POM121显著抑制HNMM细胞的迁移与侵袭。

关键词: 核孔蛋白121, 头颈部恶性黑色素瘤, 迁移, 侵袭

Abstract: Objective The aim of this study was to investigate the expression of nuclear pore membrane protein 121(POM121)in head and neck mucosal melanoma(HNMM)and its effect on the migration and invasion of HNMM cells. Methods The cancer tissues from 63 patients with HNMM and 20 adjacent normal oral epithelial tissues who were treated in the Harbin Cancer Hospital of Harbin Medical University from March 1,2011 to March 30,2015 were collected,and the expression of POM121 was detected by immunohistochemistry.The relationship between the expression of POM121 and the clinicopathological features of HNMM patients and its effect on the prognosis of patients were analyzed.Western blot was used to detect the expression of POM121 in HNMM cells(MM9H-1 cells)and human oral epithelial cells(human oral keratinocytes,HOK cells).After knocking down POM121 with small interfering RNA(siRNA),the effects on the migration and invasion of HNMM cells were detected by Scratch assay and Transwell assay. Results Immunohistochemistry showed that expression of POM121 was significantly higher in HNMM tissues than that in adjacent tissues(P＜0.001).The expression of POM121 was associated with tumor size and stage(P＜0.05).Kaplan-Meier analysis showed that the disease-free survival(DFS)and overall survival(OS)of patients with low expression of POM121 were significantly longer than those with high expression of POM121.Cox regression analysis showed that POM121 expression,AJCC stage and lymph node metastasis were influencing factors for OS and DFS of HNMM patients(P＜0.05).Western blot results showed that the expression of POM121 protein in MM9H-1 cells was significantly higher than that in HOK cells(P＜0.05).After knocking down POM121,the cell migration rate and cell invasion ability were significantly reduced(P＜0.001). Conclusion POM121 is highly expressed in HNMM tissues and cells,and down-regulation of POM121 significantly inhibits the migration and invasion of HNMM cells.

Key words: Nuclear pore membrane protein 121, Head and neck mucosal melanoma, Migration, Invasion

中图分类号:

R739

张塨钰, 郭浩兵, 鲁莉莉, 宋宁扬, 朴松林. POM121在头颈部黏膜黑色素瘤中的表达及临床意义[J]. 实用肿瘤学杂志, 2025, 39(2): 108-115.

ZHANG Gongyu, GUO Haobing, LU Lili, SONG Ningyang, PIAO Songlin. Expression and clinical significance of POM121 in head and neck mucosal melanoma[J]. Journal of Practical Oncology, 2025, 39(2): 108-115.

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POM121在头颈部黏膜黑色素瘤中的表达及临床意义

Expression and clinical significance of POM121 in head and neck mucosal melanoma

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