角化棘皮瘤与高分化鳞状细胞癌细胞周期和细胞凋亡相关蛋白表达的研究

doi:10.11904/j.issn.1002-3070.2014.06.002

Abstract

Abstract:

Objective To explore the expressions and significances of p53、bcl-2、Ki-67 and apoptosis in keratoacanthoma(KA)and well differentiated squamous cell carcinoma(WDSCC).Methods The expressions of p53、bcl-2、Ki-67 were detected by immunohistochemical technique in 44 cases of KA and 20 cases of WDSCC.Apoptosis was identified by using terminal deoxynucleotidyl transfers(TdT)-mediated dUTP-biotin nick and labelling(TUNEL)method.Results The positive expressive rates of p53 in proliferative、mature and regressive of KA were 22.23%、26.18% and 6.52%,which was lower than the positive rate of WDSCC(41.82%).Significant differences were found between every period of KA with WDSCC in p53 expression;The intensity of expression and pattern were similar in KA of Ki-67 and p53.There were a positive correlation between p53 and Ki-67 expression rate(r=0.986,P＜0.001).The bcl-2 staining showed weak expression in 1 case in WDSCC and 2 cases in KA,but only a few positive tumor cells was limited to basal cell layer in KA.The average apoptosis rate in KA was 21.72%,which was apparently higher than in WDSCC(9.925%).There were a negative correlation between the apoptosis rate and proliferation rate of Ki-67(r=-0.824,P＜0.001).Conclusion The proliferation and apoptosis in KA can coexist.However,the apoptosis will occupy a dominance position in regressive phase,which results in the regression of tumor.The expressions of p53、Ki-67 and apoptosis would be a certain significance in differentiated KA and WDSCC.

Key words: Keratoacanthoma, Squamous cell carcinoma, p53, bcl-2, Ki-67

CLC Number:

R739.5

LIU Bingmei,YANG Jing,ZHANG Hanwen,XING Ronggui,LI Lili,ZHAO Liping,QIAO Shi,YOU Haiyan. The research on expression of cell cycle and apoptosis related protein in keratoacanthoma and well-differentiated squamous cell carcinoma[J]. PRACTICAL ONCOLOGY JOURNAL, 2014, 28(6): 485-491.

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The research on expression of cell cycle and apoptosis related protein in keratoacanthoma and well-differentiated squamous cell carcinoma

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