DKK1蛋白表达与肝内胆管细胞癌患者淋巴结侵犯及其预后的相关性研究

doi:10.11904/j.issn.1002-3070.2017.03.007

Abstract

Abstract: Objective The objectives of this study were to investigate the relationship between DKK1 protein expression and lymph node invasion,and prognosis in patients with intrahepatic cholangiocarcinoma.Methods From January 2005 to December 2012,78 patients with intrahepatic cholangiocarcinoma were treated with endoscopic resection.Tissue microarray of intrahepatic cholangiocarcinoma and adjacent tissues were done and biochemical indexes were measured before operation.The patients were recruited every 3 months after operation.The Kaplan-Meier method was used to analyze the recurrence-free survival curve and the overall survival curve.The Cox proportional hazards regression model was used to analyze the multivariate analysis and to determine the factors that affect the disease-free survival(RFS)and overall survival(OS).Results There were significant differences in the expression of GGT,hepatic portal lymph node invasion,Child-Pugh grade,MMP9 and DKK1 protein in the negative and positive expression of intrahepatic cholangiocarcinoma(P＜0.05).The positive expression rate of DKK1 protein in intrahepatic cholangiocarcinoma was 35.90%(28/78),and the positive expression rate of DKK1 protein in hilar cholangiocarcinoma was 14.10%(11/78).RFS and OS in 78 cases of intrahepatic cholangiocarcinoma were 51.28%(40/78)and 50.00%(38/78),41.03%(32/78)and 38.46%(30/78),25.64%(20/78)and 23.08%(18/78)after surgery for 1,3 and 5 years,respectively.The results from univariate and multivariate analysis showed that GGT,CA19-9,CEA,tumor size,DKK1 and hilar lymph node invasion were the prognostic factors of OS in patients with intrahepatic cholangiocarcinoma.CEA,tumor size,DKK1 and hilar lymph node involvement were prognostic factors for RFS in patients with intrahepatic cholangiocarcinoma.DKK1 protein expression positive and negative OS curve or RFS curve showed that 5 years after intrahepatic cholangiocarcinoma patients DKK1 protein expression positive and negative OS were 28.20% and 20.51%;RFS were 24.36% and 21.79%.Conclusion The expression of DKK1 protein is closely related to lymph node invasion in patients with intrahepatic cholangiocarcinoma.The expression of DKK1 protein is the prognostic factor of OS and RFS in patients with intrahepatic cholangiocarcinoma.

Key words: DKK1 protein, Intrahepatic cholangiocarcinoma, Lymph node invasion, Prognosis

CLC Number:

R735.7

ZHAO Lifeng,CHENG Weihua,LEI Da,YANG Yanwei,WANG Gang. The relationship between DKK1 protein expression and lymph node invasion,and prognosis in patients with intrahepatic cholangiocarcinoma[J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(3): 228-233.

References

1 Bartsch F,Baumgart J,Heinrich S,et al.Resection of intrahepatic cholangiocarcinoma[J].HPB,2016,18(1):463-471.
2 李永胜,孙保君.肝内胆管癌手术治疗的预后影响因素分析[J].实用癌症杂志,2016,31(4):610-612.
3 陆凯,张博,董立巍,等.中下段胆管癌的预后影响因素分析[J].现代生物医学进展,2012,12(26):5068-5071.
4 王捷.对“1370例肝内胆管细胞癌肝切除术的疗效及预后因素分析”和“肝门部胆管癌手术切除的远期疗效及预后因素分析”的点评[J].中华消化外科杂志,2016,4(1):334.
5 Tian E,Zhan F,Walker R,et al.The role of the Wnt-signaling antagonist DKK1 in the development of osteolytic lesions in multiple myeloma[J].N Engl J Med,2003,349(26):2483-2494.
6 Wang JY,Zhou PR,Liu Y,et al.The analysis of DKK1 polymorphisms in relation to skeletal phenotypes and bone response to alendronate treatment in Chinese postmenopausal women[J].Pharmacogenomics,2016,17(3):209-217.
7 Ruo YS,Xin RY,Qiu JS,et al.High expression of Dickkopf-related protein 1 is related to lymphatic metastasis and indicates poor prognosis in intrahepatic cholangiocarcinoma patients after surgery[J].Cancer,2013,119(5):993-1003.
8 董辉,丛文铭.提高肝癌规范化病理诊断水平,为临床精细化治疗保驾护航-《原发性肝癌规范化病理诊断指南(2015年版)》解读[J].中国普通外科杂志,2016,25(7):939-943.
9 李玉博,高雪梅,程敬亮,等.体素内不相干运动扩散加权成像在肝细胞癌术前分级中的应用[J].中国医学影像技术,2014,34(11):1669-1673.
10 权继传,解亦斌,田艳涛.国际抗癌联盟胃癌TNM分期系统第七版解读[J].中华诊断学电子杂志,2014,2(1):59-61.
11 余杏娟,许静,郑利民.组织芯片制备及在肝癌研究中的应用体会[J].中国免疫学杂志,2013,29(10):1089-1092.
12 Nagata S,Tomoeda M,Kubo C,et al.Intraductal polypoid growth variant of pancreatic acinar cell carcinoma metastasizing to the intrahepatic bile duct 6 years after surgery:A case report and literature review[J].Pancreatology,2012,12(1):23-26.
13 张登才,刘斌,张丽华,等.一种简便实用的组织芯片制作方法[J].诊断病理学杂志,2013,20(11):722-724.
14 Kononen J,Bubendorf L,Kallioniemi A,et al.Tissue microarrays for high-throughput molecular profiling of tumor specimens[J].Nat Med,1998,4(4):844-847.
15 Yamabuki T,Takano AS,Ishikawa N,et al.Dikkopf-1 as a novel serologic and prognostic biomarker for lung and esophageal carcinomas[J].Cancer Research,2007,67(6):2517-2525.
16 Forget MA,Turcotte S,Beauseigle D,et al.The Wnt pathway regulator DKK1 is preferentially expressed in hormone-resistant breast tumours and in some common cancer types[J].Br J Cancer,2007,96(4):646-653.
17 Sheng SL,Huang GB.Clinical significance and prognostic value of serum Dickkopf-1 concentrations in patients with lung cancer[J].Clinical Chemistry,2009,55(9):1656-1664.

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The relationship between DKK1 protein expression and lymph node invasion,and prognosis in patients with intrahepatic cholangiocarcinoma

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