miR-409-5p对肝癌细胞系HepG2增殖及迁移的影响

doi:10.11904/j.issn.1002-3070.2017.04.004

Abstract

Abstract: Objective The objective of this study was to observe effects of miR-409-5p on the proliferation and migration of HepG2 cells.Methods HepG2 cells were transfected with miR-409-5p mimics and verified by Real-Time quantitative PCR.After high expression of miR-409-5p,MTT and wound healing assays were used to detect the proliferation and migration ability of HepG2 cells.Results The cell viability of HepG2 cells transfected with miR-409-5p mimics was significantly decreased in comparison with the control group and showed a dose-and time-dependent manner(P＜0.05).The migration ability of cells overexpressing miR-409-5p mimics was significantly lower than that in the NC group(P＜0.05).There was significant difference between the two groups at treatments for 24 h and 48 h.Conclusion Up-regulated miR-409-5p can inhibit the proliferation and migration of HepG2 cells.

Key words: miR-409-5p, HepG2, Proliferation, Migration

CLC Number:

R735.7

WANG Rui ,YU Chenhuan ,FANG Jie. Effects of miR-409-5p on the proliferation and migration of hepatoma HepG2 cell line[J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(4): 305-309.

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Effects of miR-409-5p on the proliferation and migration of hepatoma HepG2 cell line

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