红松松塔提取物多聚苯丙素-多糖复合物诱导人乳腺癌MCF-7细胞凋亡

doi:10.11904/j.issn.1002-3070.2019.04.001

Abstract

Abstract: Objective The mechanism of apoptosis induced by polyphenyl propenoid-polysaccharide complex(PPC)in human breast cancer MCF-7 cells was studied.Methods MCF-7 cells were cultured with different concentrations of PPC for different time.The effects of PPC on proliferation and apoptosis were detected in MCF-7 cells by MTT assay,fluorescent staining,apoptosis detection kit,DNA gel electrophoresis and Western blot.Results PPC induced apoptosis in MCF-7 cells.When apoptosis occurred,the enzyme activities of caspase-3 and-9 were increased,and the expression of pro-caspase-3 protein was decreased.Caspase-3 inhibitor(z-DEVE-fmk)could partially inhibit PPC-induced apoptosis and inhibit activation of caspase-3 precursor enzyme.Conclusion PPC induces apoptosis of MCF-7 cells by activating caspase family.

Key words: Pine Cone, Breast cancer, MCF-7 cells, Apoptosis, Caspase-3, Caspase-3 inhibitor

CLC Number:

R961
R73.36

AN Weiwei, TANG Yali, ZHAO Lianhua, YANG Yue, WANG Xinling. Polyphenylene-polysaccharide complex from pinus koraiensis extract induces apoptosis in human breast cancer MCF-7 cells[J]. Journal of Practical Oncology, 2019, 33(4): 289-293.

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Polyphenylene-polysaccharide complex from pinus koraiensis extract induces apoptosis in human breast cancer MCF-7 cells

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