PNCK对鼻咽癌细胞增殖和凋亡作用的研究

doi:10.11904/j.issn.1002-3070.2019.05.003

Abstract

Abstract: Objective The aim of this study was to explore the expression of pregnancy-upregulated nonubiquitous calmodulin kinase(PNCK)in nasopharyngeal carcinoma(NPC)and its effect on proliferation and apoptosis of NPC cells.Methods Immunohistochemistry was used to detect the expression of PNCK in NPC and normal tissues.The lentiviral vector was constructed to interfere with the expression of PNCK gene,confirming by real-time PCR and Western blot assays.The MTT assay,Annexin V staining with flow cytometry(FCM)analysis and caspase 3/7 activity assays were used to detect proliferation and apoptosis of CNE-2Z cells knocked-down PNCK.Results PNCK protein was highly expressed in NPC tissues.RT-qPCR and Western blot results confirmed that lentivirus successfully interfered with PNCK expression in nasopharyngeal carcinoma CNE-2Z cells.MTT assay result confirmed that the knock-down of PNCK significantly inhibited proliferation and induced apoptosis of CNE-2Z cells.Conclusion Interfering with PNCK gene expression inhibits proliferation of nasopharyngeal carcinoma cells and induces cell apoptosis,which can be potential targets for cancer treatment of nasopharyngeal carcinoma.

Key words: PNCK, Nasopharyngeal carcinoma, Proliferation, Apoptosis

CLC Number:

R739.62

CHEN Guanghao, FANG Jingxuan, PENG Sujuan, YAN Weiwei, WANG Fuhai, CHEN Chengzhe, CHEN Sichuan, PAN Jianji, QIU Sufang. Effects of PNCK on proliferation and apoptosis of nasopharyngeal carcinoma cells[J]. Journal of Practical Oncology, 2019, 33(5): 397-401.

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URL: http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/10.11904/j.issn.1002-3070.2019.05.003

http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2019/V33/I5/397

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Effects of PNCK on proliferation and apoptosis of nasopharyngeal carcinoma cells

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