circRNA MYO1C对肺癌细胞自噬和肿瘤干细胞分化的影响

doi:10.11904/j.issn.1002-3070.2021.06.003

Abstract

Abstract: Objective The aim of this study was to investigate the effects of circRNA MYO1C on autophagy and cancer stem cells(CSCs) differentiation of non-small cell lung cancer(NSCLC). Methods The levels of circRNA MYO1C in normal lung epithelial cells and NSCLC A549 cells were detected by RT-qPCR.A549 cells were divided into the control,NC and siMYO1C groups,and circRNA MYO1C was interfered by transfection with si-circRNA MYO1C plasmids.The cell growth viability and migration ability were detected by CCK-8 and Transwell assays.The expression of CSCs differentiation marker proteins(OCT4,SOX2 and NANOG)and autophagy marker proteins(P62 and IC3II/LC3I)was detected by Western blot. Results Compared with normal lung epithelial cells,the expression of circRNA MYO1C was significantly up-regulated in A549 cells(0.99±0.06 vs. 4.87±0.88,P=0.002).Compared with the NC group,the level of circRNA MYO1C,cell viability,cell migration,the levels of OCT4,SOX2,NANOG and IC3II/LC3I proteins in the siMYO1C group were significantly reduced,the level of P62 protein was significantly increased(P＜0.001). Conclusion Down-regulating circRNA MYO1C can inhibit CSCs differentiation and autophagy-related protein expression of NSLCLC cells,and inhibit lung cancer cell migration.

Key words: Non-small cell lung cancer, circRNA MYO1C, Cancer stem cells differentiation, Autophagy

CLC Number:

R734.2

LUO Wen, JIA Li, ZHANG Jiawen, WANG Dongjie, KONG Yingjun. The effects of circRNA MYO1C on lung cancer cell autophagy and cancer stem cell differentiation[J]. Journal of Practical Oncology, 2021, 35(6): 500-505.

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URL: http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/10.11904/j.issn.1002-3070.2021.06.003

http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2021/V35/I6/500

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The effects of circRNA MYO1C on lung cancer cell autophagy and cancer stem cell differentiation

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