PRACTICAL ONCOLOGY JOURNAL ›› 2011, Vol. 25 ›› Issue (3): 215-218.doi: 10.3969/j.issn.1002-3070.2011.03.004

Previous Articles     Next Articles

The effect of calcium-sensing receptor on the secretion of cytokine in the differentiated THP-1 cells

XI Yuhui1,LI Hongzhu1,WANG Xiuli1,MENG Qingwei2,LIN Yan1,WANG Lina1,ZHANG Li1,SHE Lixia3   

  1. 1.Department of Pathopysiology,Harbin Medical University,Harbin,150081;
    2.The Third Affiliated Hospital of Harbin Medical University,Harbin,150081;
    3.The Rangbei Hospital of Daqing First Hospital,Daqing,163000
  • Received:2011-05-11 Online:2011-03-25 Published:2012-02-21

PDF (PC)

70

Abstract: Objective To observe the function of calcium sensing receptor(CaSR)in differentiated THP-1(human acute monocytic leukemia cell line)cells.Methods In the present study,we induced and differentiated the monocyte-like THP-1 cells into macrophage-like THP-1 cells with PMA.The secretion of cytokines was analyzed by ELISA.The expression of CaSR and NF-κB in the differentiated THP-1 cells was identified by Western blotting.Results (1)We found that GdCl3 elevation stimulated both of IL-1β and TNFα release in differentiated THP-1 cells.(2)GdCl3 could increase the expression of NF-κB-p65 and decrease the expression of NF-κB-IκBα.The effects of GdCl3 were inhibited by NPS2390.Conclusion The activation of CaSR in macrophage-like THP-1 cells could commit to cytokine secretion via NF-κB signal transduction pathway.These results suggested that CaSR might be involved in a variety of pathological processes mediated by activated monocyte-macrophages.

CLC Number: