Abstract: Objective To study the correlation between serum tumor marker and immumohistochemical marker of breast cancer.Methods To determine CA15-3 and CEA in serum of 65 breast cancer before surgery by electro chemiluminescent immunoassay(ECLIA).The expressions of C-erbB2,ER,PR in 65 breast cancer tissues were detected by immunohistochemistry,and analyze the correlation of them.Results CA15-3 was positively correlated with C-erbB2(r=0.597,P=0.000),negatively correlated but no significant with ER(r=-0.190,P=0.129),and PR(r=-0.179,P=0.153);CEA was positively correlated with C-erbB2(r=0.414,P=0.001),negatively correlated with ER(r=-0.267,P=0.031),negatively correlated but no significant with PR(r=-0.157,P=0.211);The positive rate of C-erbB2 in breast cancer was increased significantly with the clinical stage increasing(P=0.038),the positive rate of CA15-3 and CEA were increased with the clinical stage increasing(P=0.077,P=0.772).Conclusions CA15-3 was positively correlated with C-erbB2.CEA was positively correlated with C-erbB2,negatively correlated with ER;the positively rate of C-erbB2 in breast cancer was increased significantly with the clinical stage increasing,the positive rate of CA15-3 and CEA were increased with the clinical stage increasing.