癌性腹水对卵巢癌细胞增殖及迁移的影响

doi:10.11904/j.issn.1002-3070.2016.01.007

Abstract

Abstract: Objective To evaluate the effects of malignant ascites on the morphological characteristics and the proliferation and migration abilities of the tumor cell and ovarian cancer cell lines(SKOV3)in the ovarian cancerous ascites.Methods Tumor cells extracted from the ovarian cancerous ascites were cultured in vitro with DMEM high glucose culture medium,and ovarian cancer cell lines(SKOV3)were cultured in DMEM high glucose and DMEM high glucose with different proportion of malignant ascites.The morphological characteristics of the cells were observed by optical microscope and electron microscope respectively.Cell proliferation ability was detected by CCK kit;The effect of SKOV3 on the migration of ovarian cancer cell lines was measured by scratch test.Results The morphological characteristics of tumor cells and ovarian cancer cell lines(SKOV3)in ovarian cancer ascites were significantly different.The proliferation ability of tumor cells was decreased without the ascites.The proliferation and migration abilities of SKOV3 cultured in mixed culture medium were significantly improved compared with the cells cultured in high glucose medium.Conclusion The change of tumor cell morphology in ascites benefits its abilities of proliferation and migration.The malignant ascites promote the abilities of proliferation and migration of ovarian cancer cell line(SKOV3).

Key words: Ovarian cancer, Cancerous ascites, Ovarian cancer cell line(SKOV3), Proliferation, Migration

CLC Number:

R737.31

GUO Shuang, LIU Duanyang, LI Jing, LI Jiade, JIN Xiaoming. The effect of malignant ascites on the proliferation and migration of ovarian cancer cells[J]. PRACTICAL ONCOLOGY JOURNAL, 2016, 30(1): 36-40.

References

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The effect of malignant ascites on the proliferation and migration of ovarian cancer cells

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