RPL23-siRNA干扰片段的构建及筛选

doi:10.11904/j.issn.1002-3070.2016.06.002

Abstract

Abstract: Objective To construct and screen out the RPL23-siRNA interference fragments,providing the basis for the following experiments about the correlation with RPL23 and gastric cancer.Methods The RPL23-siRNA,synthesized chemically through lipofection,were selected from three target sequences by RNA interference and detected by real-time PCR and Western blot.Results Compared with normal cell group and RPL23 control group,the mRNA and protein expression of RPL23 in the other 3 interference groups were significantly decreased(P＜0.01).Multiple comparisons showed that the interference efficiency of RPL23-siRNA1 group was significantly higher than that of RPL23-siRNA2 group and RPL23-siRNA3 group(P＜0.01).Conclusion The RPL23-siRNA interference fragment can be successfully constructed and screened out,which provides the basis for the following experiments.

Key words: RPL23, siRNA, RNAi

CLC Number:

R735.2

PENG Wenmiao,ZHANG Zhimin,HU Meng,YU Lifang,ZHANG Bicheng,RAO Zhiguo,QIN Chuanrong. Construction and screening of RPL23-siRNA interference fragments[J]. PRACTICAL ONCOLOGY JOURNAL, 2016, 30(6): 487-491.

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Construction and screening of RPL23-siRNA interference fragments

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