Abstract: Objective To investigate the role of glycogen synthase kinase-3beta(GSK3β)on invasion and metastasis of human osteosarcoma cells.Methods Expression and phosphorylation of GSK3β were examined in osteosarcoma cell lines and tumor tissue from osteosarcoma patients by Western blot.The effects of small molecule GSK3βinhibitors on cell metastasis were detected by cell invasion assay and cell migration assay.Results Osteosarcoma cell lines showed increased GSK3β expression and abnormal activity regulation.In tumor tissue of patients with osteosarcoma metastasis and non-metastasis,GSK3β were detected expression and abnormal activity,especially in tumor tissue of the patients with osteosarcoma metastasis.Inhibition of GSK3β activity resulted in inhibiting cells invasion and migration in osteosarcoma cell line.Conclusion In this research,we demonstrated that GSK3β encouraged osteosarcoma cells metastasis.The result will open up a potential target for clinical treatment of osteosarcoma.

Key words: Osteosarcoma, GSK3β, Migration, Invasion