Journal of Practical Oncology ›› 2025, Vol. 39 ›› Issue (1): 13-20.doi: 10.11904/j.issn.1002-3070.2025.01.003

• Basic Research • Previous Articles     Next Articles

Effect and mechanism of CXCL8 on the paclitaxel sensitivity of cervical cancer cells

ZHANG Hua1, Nurbia Ibibulla1, LU Pengfei1, JIA Chunli1, PEI Xuanxuan2, BAO Yongxing1   

  1. 1. Department of Oncology II,The First Affiliated Hospital of Xinjiang Medical University,State Key Laboratory of Pathogenesis,Prevention and Treatment of High Incidence Diseases in Central Asia,Urumqi 830000,China;
    2. Zhongshan School of Medicine,Sun Yat-sen University
  • Received:2024-08-02 Revised:2024-12-23 Online:2025-02-28 Published:2025-03-19

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Abstract: Objective The Objective of this study was to explore the effect of knocking down CXCL8 on the efficacy of paclitaxel chemotherapy in cervical squamous cell carcinoma and to investigate its potential mechanism of action. Methods The Hela cell model was used to specifically inhibit CXCL8 gene expression through lentivirus-mediated RNA interference(RNAi)technology.The optimal transfection conditions were HitransG P and a multiplicity of infection(MOI)of 100.The CCK-8 assay was used to screen the optimal intervention concentration of puromycin as 1.5 μg/mL.The LV-CXCL8-RNAi(3 targets)and negative control lentivirus were transfected into cells under optimal transfection conditions and set up a blank control group.The qRT-PCR assay was used to select the sh-CXCL8-13 group lentivirus as the intervention sequence and virus for subsequent experiments.The experiments were divided into the blank control group,negative control group,sh-CXCL8 group,paclitaxel group,and sh-CXCL8+paclitaxel group.The proliferative activity and invasive ability of cervical cancer cells were assessed by CCK-8 and cell invasion assays.The expression of CXCL8,Bcl2,Bax,and β-actin were detected by qRT-PCR and Western blot. Results Compared with the other four groups,the proliferative and invasive ability of Hela cells was significantly reduced in the sh-CXCL8+paclitaxel group,and the difference was statistically significant(P＜0.01).The qRT-PCR results showed that the expression of CXCL8,Bcl2,PIK3CB,and Akt1 genes was significantly reduced,and the expression of Bax gene was significantly increased in the sh-CXCL8+paclitaxel group.The difference between the groups was statistically significant(P＜0.001).The results of Western blot showed that the expression of CXCL8,PIK3CB,and p-Akt1 proteins was reduced in the sh-CXCL8+paclitaxel group(P＜0.05). Conclusion Knocking down CXCL8 can reduce the proliferative and invasive capacity of Hela cells,possibly by affecting the PI3K/Akt pathway to affect the drug sensitivity of Hela cells to paclitaxel.

Key words: Cervical cancer, CXCL8, Paclitaxel, Sensitivity, Invasion

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