美登木素对人甲状腺癌细胞增殖、侵袭、迁移、凋亡和自噬的影响

doi:10.11904/j.issn.1002-3070.2025.01.004

Abstract

Abstract: Objective The Objective of this study was to investigate the effects of maytansine on proliferation,migration,invasion,apoptosis and autophagy of human thyroid cancer C643 cells. Methods C643 cells were treated with different concentrations(0.049,0.195,0.781,3.125,12.5,50 and 200 μmol/L)of maytansine,the effect of maytansine on the proliferation of C643 cells was detected by the sulforhodamine B(SRB)method,and the concentration of subsequent experiments was determined.C643 cells in the logarithmic growth stage period were divided into the control group,low-dose group,mid-dose group and high-dose group.The effects of maytansine on migration and invasion abilities of C643 cells were detected by cell scratch and Transwell chamber assay;The levels of reactive oxygen species(ROS)were detected by 2 ′,7 ′-dichlorofluorescein diacetate(DCFH-DA)fluorescent probe experiment;The apoptosis rate of C643 cells was detected by flow cytometry;The expression of proteins related to apoptosis or autophagy was detected by Western blot. Results Maytansine at concentrations of 0.049,0.195,0.781,3.125,12.5,50 and 200 μmol/L could inhibit the proliferation of C643 cells(P＜0.05),and exhibited a significant concentration time dependence.The half maximal inhibitory concentrations(IC₅₀)at 24,48 and 72 h were 54.255,5.193 and 0.647 μmol/L,respectively;The cell scratch and Transwell chamber results showed that maytansine at concentrations of 0.1,1 and 10 μmol/L could reduce the migration and invasion abilities of C643 cells(P＜0.05 and P＜ 0.01).The fluorescence probe results showed that maytansine at concentrations of 0.1,1 and 10 μmol/L could increase the intracellular ROS levels of C643 cells(P＜0.01).The flow cytometry results showed that maytansine at concentrations of 0.1,1 and 10 μmol/L could concentration dependently increase the apoptosis rate of C643 cells(P＜0.01).The Western blot results showed that with the increase of maytansine concentrations,the expression of Bax protein related to apoptosis in C643 cells increased(P＜0.05),the expression of Bcl-2 decreased(P＜0.05),the expression of LC3II/I(P＜0.05)and Beclin-1(P＜0.01)increased,while the expression of p62 decreased(P＜0.001). Conclusion Maytansine can inhibit the proliferation,migration and invasion of human thyroid cancer C643 cells,and induce the synergistic effect on apoptosis and autophagy by increasing intracellular ROS levels.

Key words: Maytansine, Human thyroid cancer cells, Cell apoptosis, Autophagy

CLC Number:

R736.1

GU Na, TAN Cheng, WANG Luyao, SHEN Hongsheng, LI Xijing, LI Xinyan, LIU Cuicui, ZHAO Xiumei. Effects of maytansine on proliferation,migration,invasion,apoptosis and autophagy of human thyroid cancer cells[J]. Journal of Practical Oncology, 2025, 39(1): 21-29.

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URL: http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/10.11904/j.issn.1002-3070.2025.01.004

http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2025/V39/I1/21

References

1 施晓辉,白云峰,塔拉,等.CCT各亚基在甲状腺癌中的表达及其对患者临床预后、肿瘤微环境和化疗药物敏感性的影响[J].中国肿瘤生物治疗杂志,2024,31(9):895-906.
2 刘翠翠,顾娜,王璐瑶,等.美登木素对人乳头状甲状腺癌B-CPAP细胞增殖和凋亡的作用及机制[J].现代药物与临床,2023,38(11):2655-2659.
3 宁爱兰,潘琼婧,方肇寅,等.细胞松弛素D及美登木素对ECa-109细胞形态影响的研究——扫描电镜观察[J].解剖学报,1979,10(1):96-100.
4 Yu XL,Wu HC,Hu HY,et al.Zein nanoparticles as nontoxic delivery system for maytansine in the treatment of non-small cell lung cancer[J].Drug delivery,2020,27(1):100-109.
5 韩伟,安靓.美登木素抑制鼻咽癌的增殖以及侵袭迁移机制的生物信息学分析[J].实用医学杂志,2017,33(8):1202-1207.
6 Xie HN,Chen YY,Zhu GB,et al.Targeted delivery of maytansine to liver cancer cells via galactose-modified supramolecular two-dimensional glycomaterial[J].Chem Commun(Camb),2022,58(32):5029-5032.
7 Liu JX,Wu YY,Meng S,et al.Selective autophagy in cancer:mechanisms,therapeutic implications,and future perspectives[J].Molecular Cancer,2024,23:22.
8 Ariosa AR,Ahiri V,Lei Y,et al.A perspective on the role of autophagy in cancer[J].Biochim Biophys Acta Mol Basis Dis,2021,1867(12):166262.
9 Jomon S,Krishnan R.Microtubules and cell division:potential pharmacological targets in cancer therapy[J].Curr Drug Targets,2023,24(11):889-918.
10 胡嘉芮,樊捷婷,何晓华,等.紫杉醇致周围神经病变的研究进展[J].中国新药与临床杂志,2023,42(10):616-621.
11 Sameen Z,Muhammad A,Khushbukhat K,et al.New insights into the anticancer therapeutic potential of maytansine and its derivatives[J].Biomed Pharmacother,2023,9(165):115039.
12 Cao S,Dong YH,Wang F,et al.Tubulin maytansine site binding ligands and their applications as MTAs and ADCs for cancer therapy[J].Curr Med Chem,2020,27(27):4567-4576.
13 曹春艳,张家祺,张立超.澳洲茄边碱抗肿瘤作用及其机制研究进展[J].中国医院药学杂志,2022,42(20):2188-2194.
14 Hardeep ST,Jagjit K,Kanupriya V,et al.Molecular mechanisms behind ROS regulation in cancer:A balancing act between augmented tumorigenesis and cell apoptosis[J].Arch Toxicol,2023,97(1):103-120.
15 Muhammad JI,Ayesha K,Zaighum A,et al.Interplay of oxidative stress,cellular communication and signaling pathways in cancer[J].Cell Commun Signal,2024,22(1):7.
16 Sahoo BM,Banik BK,Borah P,et al.Reactive Oxygen Species(ROS):key components in cancer therapies[J].Anticancer Agents Med Chem,2022,22(2):215-222.
17 陈建海,李惠斌.荜茇酰胺抗肿瘤机制的研究进展[J].现代肿瘤医学,2024,32(3):583-588.
18 周欢思,李艳萍,金明静,等.消瘤藤乙酸乙酯提取物对结直肠癌细胞增殖、凋亡及Bax/Bcl-2/Caspase-3信号通路的影响[J].现代肿瘤医学,2024,32(10):1767-1773.
19 秦丽,栾振先,李敏,等.黄芪-丹参通过PI3K/Akt/mTOR 通路调控自噬改善大鼠急性肺损伤[J].中国中药杂志,2024,49(12):1-7.
20 Chen RC,Zou J,Zhong X,et al.HMGB1 in the interplay between autophagy and apoptosis in cancer[J].Cancer Lett,2024,581(28):216494.
21 Maurizio S.Crosstalk of autophagy and apoptosis[J].Cells,2022,11(9):1479.

[1]	LUO Wen, JIA Li, ZHANG Jiawen, WANG Dongjie, KONG Yingjun. The effects of circRNA MYO1C on lung cancer cell autophagy and cancer stem cell differentiation [J]. Journal of Practical Oncology, 2021, 35(6): 500-505.
[2]	HU Qihui, DU Yichao, HE Kai, FU Wenguang, XIA Xianming. The role of endoplasmic reticulum stress in liver cancer [J]. Journal of Practical Oncology, 2021, 35(2): 160-164.
[3]	HUANG Li, LONG Youmei, FU Yixia, XIA Liangbin. Effect of pterostilbene on apoptosis and glycolysis of ovarian cancer SKOV3 cells and its mechanism [J]. Journal of Practical Oncology, 2019, 33(6): 502-507.
[4]	LIU Eryu, YANG Hao, LIANG Guohua, ZHAO Yanfang, ZHAO Wenhui. Action for CDK4/6 inhibitors combined with mTOR inhibitors in breast cancer and its mechanism [J]. Journal of Practical Oncology, 2019, 33(5): 476-480.
[5]	BIAN Xiusen, LI Guang, GUAN Xinyu, ZHANG Yi, DI Chang, MA Can. Molecular mechanism of UHRF1 inhibiting proliferation of lung adenocarcinoma cells by regulating autophagy [J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(6): 498-502.
[6]	LIU Yajing, LIU Guoyu, WANG Shubin, FANG Zhengyu. Effect of salinomycin on proliferation and autophagic flow of human malignant melanoma M21 cells [J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(3): 203-207.
[7]	ZHANG Pei, CAO Shoubo, YU Yan. Research progress of autophagy-mediated regulation of tumor associated macrophages [J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(2): 130-134.
[8]	LIANG Yusi, TAN Yonggang, ZOU Huawei. Research progress on mechanism of low-dose chemotherapy [J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(6): 538-542.
[9]	ZHANG Xuliang,HU Yahua,HUANG Danqing. Sulindac enhances sensitization effect of NF-κB on apoptosis induced by TNF-α in human breast cancer [J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(2): 102-106.
[10]	YANG Yi,DING Yan,YU Aiqing,YAN Shirong. Effects of autophagy on the proliferation and migration of cervical cancer cells [J]. PRACTICAL ONCOLOGY JOURNAL, 2016, 30(6): 481-486.
[11]	REN Tingting,XING Ying,MENG Qingwei. Progress in association between autophagy and cancer [J]. PRACTICAL ONCOLOGY JOURNAL, 2016, 30(6): 543-546.
[12]	FU Shuang, LIU Guojun. The effect of microRNA-204 on autophagy in U251 [J]. PRACTICAL ONCOLOGY JOURNAL, 2016, 30(1): 23-27.
[13]	GONG Haiyan, ZHANG Kun, LI Ling, WANG Tianjiao, WU Jin. The expression of microRNA-216a in breast cancer and the effect on autophagy [J]. PRACTICAL ONCOLOGY JOURNAL, 2015, 29(6): 507-511.
[14]	WANG Zetao,LV Fulong,WANG Jinsong. Autophagy facilitates breast cancer cells to resist tamoxifen by p38/c-Jun pathway [J]. PRACTICAL ONCOLOGY JOURNAL, 2015, 29(1): 22-28.
[15]	ZHAO Yun,WEN Feng,ZHAO Xiangxuan,GUO Qiyong. Research progress on TRAIL and its related apoptosis regulating mechanism in cholangiocarcinoma [J]. PRACTICAL ONCOLOGY JOURNAL, 2015, 29(1): 49-52.

Effects of maytansine on proliferation,migration,invasion,apoptosis and autophagy of human thyroid cancer cells

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