乙肝病毒蛋白和基因在肝内和肝外胆管癌组织中的检测及意义

doi:10.11904/j.issn.1002-3070.2017.01.002

Abstract

Abstract:

Objective To investigate the importance of hepatitis B virus(HBV)infection in the pathogenesis of cholangiocarcinoma(CC)and further clarify the correlation between the occurrence of intrahepatic and extrahepatic cholangiocarcinoma.Methods HBV protein and gene of 52 formalin fixed,paraffin embedded tissues with CC were detected by immunohistochemistry and nest PCR.Results Hepatitis B virus X gene was detectable in 33.3%(7/21)of 21 intrahepatic cholangiocarcinoma cases.Hepatitis B surface antigen(HBsAg)was detectable in 21.7%(5/21)and hepatitis B core antigen(HBcAg)was detectable in 19.0%(4/21)of 21 intrahepatic cholangiocarcinoma cases.In contrast,no HBsAg,HBcAg and hepatitis B virus X gene were detected in any of the 31 extrahepatic cholangiocarcinoma cases.Conclusion HBV infection is a significant risk factor for intrahepatic cholangiocarcinoma,but not for extrahepatic cholangiocarcinoma,in Northeast of China.The integration of HBV gene may be involved in the carcinogenesis of intrahepatic bile duct epithelial cells.

Key words: Cholangiocarcinoma, Hepatitis B virus, Intrahepatic cholangiocarcinoma, Extrahepatic cholangiocarcinoma

CLC Number:

R730.43

WU Yiqi, LUI Ying, ZHAO Li, JIN Xiaoming. Detection of hepatitis B virus protein and gene in intrahepatic and extrahepatic cholangiocarcinoma tissue[J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(1): 7-12.

References

1 Sempoux C,Jibara G,Ward SC,et al.Intrahepatic cholangiocarcinoma:new insights in pathology[J].Semin Liver Dis,2011,31(1):49-60.
2 Khan SA,Thomas HC,Davidson BR,et al.Cholangiocarcinoma[J].Lancet,2005,366(9493):1303-1314.
3 Lazaridis KN,Gores GJ.Cholangiocarcinoma[J].Gastroenterology,2005,128(6):1655-1667.
4 Khan SA,Davidson BR,Goldin RD,et al.Guidelines for the diagnosis and treatment of cholangiocarcinoma:an update[J].Gut,2012,61(12):1657-1669.
5 Tyson GL,El-Serag HB.Risk factors for cholangiocarcinoma[J].Hepatology,2011,54(1):173-184.
6 Tanaka M,Tanaka H,Tsukuma H,et al.Risk factors for intrahepatic cholangiocarcinoma:a possible role of hepatitis B virus[J].J Viral Hepat,2010,17(10):742-748.
7 Palmer WC,Patel T.Are common factors involved in the pathogenesis of primary liver cancers? A meta-analysis of risk factors for intrahepatic cholangiocarcinoma[J].J Hepatol,2012,57(1):69-76.
8 Ralphs S,Khan SA.The role of the hepatitis viruses in cholangiocarcinoma[J].J Viral Hepat,2013,20(5):297-305.
9 Shaib YH,El-Serag HB,Davila JA,et al.Risk factors of intrahepatic cholangiocarcinoma in the United States:a case control study[J].Gastroenterology,2005,128(3):620-626.
10 Khan SA,Emadossadaty S,Ladep NG,et al.Rising trends in cholangiocarcinoma:is the ICD classification system misleading us?[J].J Hepatol,2012,56(4):848-854.
11 Chaiteerakij R,Yang JD,Harmsen WS,et al.Risk factors for intrahepatic cholangiocarcinoma:association between metformin use and reduced cancer risk[J].Hepatology,2013,57(2):648-655.
12 Matsumoto K,Onoyama T,Kawata S,et al.Hepatitis B and C virus infection is a risk factor for the development of cholangiocarcinoma[J].Intern Med,2014,53(7):651-654.
13 Wu ZF,Yang N,Li DY,et al.Characteristics of intrahepatic cholangiocarcinoma in patients with hepatitis B virus infection:clinicopathologic study of resected tumours[J].J Viral Hepat,2013,20(5):306-310.
14 Zhou HB,Wang H,Li YQ,et al.Hepatitis B virus infection:a favorable prognostic factor for intrahepatic cholangiocarcinoma after resection[J].World J Gastroenterol,2011,17(10):1292-1303.
15 Kobayashi M,Ikeda K,Saitoh S,et al.Incidence of primary cholangiocellular carcinoma of the liver in Japanese patients with hepatitis C virus-related cirrhosis[J].Cancer,2000,88(11):2471-2477.
16 Donato F,Gelatti U,Tagger A,et al.Intrahepatic cholangiocarcinoma and hepatitis C and B virus infection,alcohol intake,and hepatolithiasis:a case-control study in Italy[J].Cancer Causes Control,2001,12(10):959-964.
17 Palmer WC,Patel T.Are common factors involved in the pathogenesis of primary liver cancers? A meta-analysis of risk factors for intrahepatic cholangiocarcinoma[J].J Hepatol,2012,57(1):69-76.
18 Peng NF,Li LQ,Qin X,et al.Evaluation of risk factors and clinicopathologic features for intrahepatic cholangiocarcinoma in Southern China:a possible role of hepatitis B virus[J].Ann Surg Oncol,2011,18(5):1258-1266.
19 Perumal V,Wang J,Thuluvath P,et al.Hepatitis C and hepatitis B nucleic acids are present in intrahepatic cholangiocarcinomas from the United States[J].Hum Pathol,2006,37(9):1211-1216.
20 Zhou YM,Cao L,Li B,et al.Expression of HBx protein in hepatitis B virus-infected intrahepatic cholangiocarcinoma[J].Hepatobiliary Pancreat Dis Int,2012,11(5):532-535.
21 Komuta M,Govaere O,Vandecaveye V,et al.Histological diversity in cholangiocellular carcinoma reflects the different cholangiocyte phenotypes[J].Hepatology,2012,55(6):1876-1888.
22 Theise ND,Yao JL,Harada K,et al.Hepatic 'stem cell' malignancies in adults:four cases[J].Histopathology,2003,43(3):263-271.
23 Gouasda V,Ortizcuar S.TP53R249S mutation,genetic variations in HBX and risk of hepatocellular carcinoma in The Gambia[J].Carcinogenesis,2012,33(6):1219-1224.
24 Qu ZL,Zou SQ,Cui NQ,et al.Upregulation of human telomerase reverse transcriptase mRNA expression by in vitro transfection of hepatitis B virus X gene into human hepatocarcinoma and cholangiocarcinoma cells[J].World J Gastroenterol,2005,11(36):5627-5632.
25 Assar S,Arababadi MK,Ahmadabadi BN,et al.Occult hepatitis B virus(HBV)infection:a global challenge for medicine[J].Chin Lab,2012,58(11/12):1225-1230.

[1]	LIU Nan ,HAN Zhiyang ,LU Pengtian ,LI Danyang ,WANG Yingwei ,DONG Xianghui ,ZHU Hong. Expression of polycomb protein SUZ12 predicts poor prognosis and response of patients with intrahepatic cholangiocarcinoma to adjuvant gemcitabine-based chemotherapy [J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(4): 321-328.
[2]	ZHAO Lifeng,CHENG Weihua,LEI Da,YANG Yanwei,WANG Gang. The relationship between DKK1 protein expression and lymph node invasion,and prognosis in patients with intrahepatic cholangiocarcinoma [J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(3): 228-233.
[3]	LIU Qinlan,YAN Xiaojie,XIA Xueming,BAI Li. Analysis of the clinical characteristics and its prognostic factors for hilar cholangiocarcinoma with exairesis [J]. PRACTICAL ONCOLOGY JOURNAL, 2015, 29(3): 203-207.
[4]	ZHANG Yi, QIU Guo qiang, PENG Zhihai. Expression of TRADD and NF-κB in hepatocellular carcinoma and liver cirrhosis after HBV-infected [J]. PRACTICAL ONCOLOGY JOURNAL, 2009, 23(6): 535-537.

Detection of hepatitis B virus protein and gene in intrahepatic and extrahepatic cholangiocarcinoma tissue

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 4

Recommended Articles

Metrics

Comments