Abstract: Objective The aim of this study was to investigate the effects and mechanisms of Volerovel on the proliferation and apoptosis of ovarian cancer SKOV3 cells. Methods Human ovarian cancer SKOV3 cells were treated with the negative control,low,middle and high doses of Vorinostat.The proliferation of SKOV3 cells was determined by CCK-8 assay.Western blot was used to detect the expression of H3K4ac and H3K27ac protein in SKOV3 cells.Real-time PCR was used to detect the expression of Bax,Bcl-2,p53 and Cyclin D1 in SKOV3 cells. Results Vorinostat at low,middle and high dose groups was significantly decreased proliferation of SKOV3 cells when compared to the blank group.They showed a statistical difference.As compared with the blank group,Vorinostat in the low,medium and high dose groups significantly decreased the expression of Bcl-2 mRNA and CyclinD1 mRNA,and significantly increased the expression of Bax mRNA and p53 mRNA in SKOV3 cells.Vorinostat significantly increased the expression of H3K4ac and H3K27ac protein in SKOV3 cells when compared to the negative control group. Conclusion Vorinostat can inhibit the proliferation of SKOV3 cells and promote apoptosis.The mechanism may be related to inhibition of histone deacetylase activity and activation of p53 apoptosis pathway.

Key words: Ovarian cancer SKOV3 cell, Histone deacetylase inhibitor, Vorinostat, p53 pathway