过表达SOX7对胆囊癌GBC-SD细胞增殖生物学特性的影响及其机制研究

doi:10.11904/j.issn.1002-3070.2018.04.002

Abstract

Abstract: Objective The aims of the study were to explore effects of SOX7(Sex determining region Y-box 7)overexpression on proliferation and apoptosis of gallbladder carcinoma of GBC-SD cells and the possible signal pathways involved. Methods The specific recombinant plasmid-pcDNA3.1-SOX7 was used as the experimental group to transfect GBC-SD cells and then establish a stable over-expressed SOX7 cell line.The empty vector pc-DNA3.1-mock was used as a control group.Effects of SOX7 overexpression on the proliferation of GBC-SD cells were detected by Edu cell immunofluorescent staining assay.The apoptosis of GBC-SD cells were detected by Hoechst 33342 staining assay.The phosphorylation of Akt and PTEN protein related to signaling pathways were examined by Western blot. Results The relative expression of SOX7 mRNA in the experimental group was(353±28.1)%,which was significantly increased when compared to the control group(100±2.3)%(P＜0.05).The absorbance at 450 nm in the control group was 4.6±0.4,which was significantly higher than that in the experimental group(2.4±0.2)(P＜0.05).The proportion of cell proliferation in the control group(33.3±3.4)% was higher than that in the experimental group(12.2±4.2)% in GBC-SD cells.They showed a statistically significance(P＜0.05).The proportion of apoptosis in the experimental group(10.2±2.4)% was higher than that in the control group(5.2±1.3)% in GBC-SD cells.They also showed a statistically significance(P＜0.05).The relative expression level of PTEN protein in the experimental group(0.7±0.04)significantly increased in GBC-SD cells in comparison with the control group(0.2±0.02)(P＜0.05).The relative expression of phosphorylated Akt protein in the experimental group(0.4±0.02)significantly decreased in GBC-SD cells when compared to the control group(0.8±0.03)(P＜0.05). Conclusion The overexpression of SOX7 significantly inhibits the proliferation and induces apoptosis of GBC-SD cells.Activation of the PTEN/Akt signaling pathway may involve in this process.

Key words: SOX7, Gallbladder carcinoma, PTEN, Proliferation

CLC Number:

R73.3

CHEN Zhaohong,HE Yingying,ZHANG Youcai,LU Dingyu. Effects of SOX7 overexpression on proliferation and other biological characteristics of gallbladder carcinoma GBC-SD cells and its mechanism[J]. PRACTICAL ONCOLOGY JOURNAL, 2018, 32(4): 293-297.

References

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Effects of SOX7 overexpression on proliferation and other biological characteristics of gallbladder carcinoma GBC-SD cells and its mechanism

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