丹参酚酸B调控ROS抑制大肠癌细胞HCT-116增殖并促进其凋亡

doi:10.11904/j.issn.1002-3070.2020.06.005

Abstract

Abstract: Objective The aims of this study were to verify that Salvianolic acid B(SalB)inhibited the proliferation of human colorectal cancer HCT-116 cells and promoted its apoptosis,and further elaborated its possible mechanism through reactive oxygen species(ROS). Methods The experiment was divided into four groups:HCT-116 group,HCT-116+H202 group,HCT-116+SalB group,and HCT-116+SalB+N-acetylcysteine(NAC)group.After group intervention,MTT assay was used to detect cell viability,plate colony formation assay to detect cell proliferation,and flow cytometry to detect ROS content,cell cycle and apoptotic rate. Results SalB had an inhibitory effect on HCT-116 cells,and it was a positively correlated within a certain concentration range(P＜0.01);SalB and H₂O₂ promoted the generation of ROS in HCT-116 cells(P＜0.01),and pretreatment with ROS scavenger NAC could eliminate ROS produced by SalB(P＜0.01);SalB inhibited the proliferation of HCT-116 cells(P＜0.01)and promoted apoptosis(P＜0.01).This effect could be partially reversed by NAC(P＜0.05);SalB caused cycle arrest at the G0/G1 phase in HCT-116 cells(P＜0.01),and NAC pretreatment completely reversed the cell cycle arrest induced by SalB(P＜0.05). Conclusion SalB can cause cell cycle arrest by increasing the level of ROS in HCT-116 cells,thereby inhibiting cell proliferation and promoting cell apoptosis.

Key words: Salvianolic acid B, Reactive oxygen species, Colorectal cancer, Proliferation, Apoptosis

CLC Number:

R735.3+4

GUO Piaoting, MIN Li, CHEN Jianjun, WANG Xin, NI Siyi. Salvianolic acid B regulates ROS to inhibit the proliferation and promote apoptosis in colorectal cancer HCT-116 cells[J]. Journal of Practical Oncology, 2020, 34(6): 504-510.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/10.11904/j.issn.1002-3070.2020.06.005

http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2020/V34/I6/504

References

1 Siegel RL,Miller KD,Jemal A.Cancer statistics,2020[J].CA Cancer J Clin,2020,70(1):7-30.
2 周跃,周燕红,彭莉.中药治疗大肠癌的研究进展[J].湖北科技学院学报(医学版),2019,33(5):457-460.
3 Lai E,Liscia N,Donisi C,et al.Molecular-biology-driven treatment for metastatic colorectal cancer[J].Cancers(Basel),2020,12(5):1214.
4 Pfeiffer P,Köhne CH,Qvortrup C.The changing face of treatment for metastatic colorectal cancer[J].Expert Rev Anticancer Ther,2019,19(1):61-70.
5 李淼,陈雷,胡兵.中医药治疗大肠癌转化研究[J].现代中西医结合杂志,2020,29(15):1698-1703.
6 林卉,吴木莲,金军.中医药治疗大肠癌用药规律分析[J].中医药导报,2020,26(4):94-97.
7 Wang Y,Liu P,Fang Y,et al.The effect of long-term traditional chinese medicine treatment on survival time of colorectal cancer based on propensity score matching:a retrospective cohort study[J].Evid Based Complement Alternat Med,2020,2020:7023420.
8 Guo PT,Wang PS,Liang W,et al.Salvianolic acid B reverses multidrug resistance in HCT-8/VCR human colorectal cancer cells by increasing ROS levels[J].Mol Med Rep,2017,15(2):724-730.
9 Guo PT,Wu SF,Wang JC,et al.Reverse effect of Salvianolic acid B on multidrug resistance in nude mice bearing human colon cancer stem cells[J].Mol Med Rep,2018,18(2):1323-1334.
10 Wu P,Yu T,Wu J.Licochalcone a induces ROS-mediated apoptosis through TrxR1 inactivation in colorectal cancer cells[J].Biomed Res Int,2020,2020:5875074.
11 石焕焕,朱运峰.活性氧在肿瘤发生和治疗中的作用研究进展[J].中国药理学与毒理学杂志,2019,33(5):366-372.
12 Zhou JL,Huang XY,Qiu HC,et al.SSPH I,a novel anti-cancer saponin,inhibits autophagy and induces apoptosis via ROS accumulation and ERK1/2 signaling pathway in hepatocellular carcinoma cells[J].Onco Targets Ther,2020,13:5979-5991.
13 Yin W,Zhong G,Fan H,et al.SophoraThe effect of compound on fluorouracil and oxaliplatin resistance in colorectal cancer cells[J].Evid Based Complement Alternat Med,2019,2019:7564232.
14 Wang X,Saud SM,Wang F,et al.Protective effect of ShaoYao decoction on colitis-associated colorectal cancer by inducing Nrf2 signaling pathway[J].J Ethnopharmacol,2020,252:112600.
15 Bai JH,Xu J,Zhao J,et al.Ganoderma lucidum polysaccharide enzymatic hydrolysate suppresses the growth of human colon cancer cells via inducing apoptosis[J].Cell Transplant,2020,29:963689720931435.
16 Wang R,Liu H,Shao Y,et al.Sophoridine inhibits human colorectal cancer progression via targeting MAPKAPK2[J].Mol Cancer Res,2019,17(12):2469-2479.
17 卢文杰,曹建春,李慧苹,等.早期大肠癌及癌前病变中医证候分布规律研究[J].浙江中医杂志,2019,54(7):488-489.
18 徐晓华,林佳敏,李可欣,等.从气血理论简析大肠癌的中医药治疗[J].中医杂志,2019,60(8):656-659.
19 Chen B,Huang C,Zhang Y,et al.Salvia bowleyana Dunn root is a novel source of salvianolic acid B and displays antitumor effects against gastric cancer cells[J].Oncol Lett,2020,20(1):817-827.
20 Katary MA,Abdelsayed R,Alhashim A,et al.Salvianolic acid B slows the progression of breast cancer cell growth via enhancement of apoptosis and reduction of oxidative stress,inflammation,and angiogenesis[J].Int J Mol Sci,2019,20(22):5653.
21 Sha W,Zhou Y,Ling ZQ,et al.Antitumor properties of Salvianolic acid B against triple-negative and hormone receptor-positive breast cancer cells via ceramide-mediated apoptosis[J].Oncotarget,2018,9(91):36331-36343.
22 Wu Q,Deng J,Fan D,et al.Ginsenoside Rh4 induces apoptosis and autophagic cell death through activation of the ROS/JNK/p53 pathway in colorectal cancer cells[J].Biochem Pharmacol,2017,148:64-74.
23 Li S,Cheng B,Hou L,et al.Dioscin inhibits colon cancer cells′ growth by reactive oxygen species-mediated mitochondrial dysfunction and p38 and JNK pathways[J].Anticancer Drugs,2018,29(3):234-242.
24 赵晨璐,高银亮,张国庆.活性氧在七氟烷致人口腔鳞状细胞癌凋亡中的作用研究[J].中国医药生物技术,2019,14(5):421-429.
25Yue TH,Zuo S,Bu DF,et al.Aminooxyacetic acid(AOAA)sensitizes colon cancer cells to oxaliplatin via exaggerating apoptosis induced by ROS[J].J Cancer 2020,11(7):1828-1838.
26 An N,Zheng B.MiR-203a-3p inhibits pancreatic cancer cell proliferation,EMT,and apoptosis by regulating SLUG[J].Technol Cancer Res Treat,2020,19:1533033819898729.

[1]	NIU Ming, YU Ge. Gankyrin promotes tumor proliferation,metastasis and cancer stem cell characteristic in breast cancer [J]. Journal of Practical Oncology, 2020, 34(6): 518-523.
[2]	GUO Piaoting, ZOU Yang. LncRNA SOX2OT sponge adsorbs miR-34a to promote SOX2 expression and enhance multidrug resistance in colorectal cancer [J]. Journal of Practical Oncology, 2020, 34(5): 398-403.
[3]	HAN Jing, PU Yan, LIU Huibin. Effects of SKA1 on cell proliferation and apoptosis of renal cell carcinoma and its molecular mechanism [J]. Journal of Practical Oncology, 2020, 34(5): 404-411.
[4]	DONG Manku, JIANG Fuquan, LIN Haiguan, SUN Xiaomin, LIU Jingxin, HU Gang, YANG Jianwu. LncRNA BLACAT1 targeting LEMD1 promotes the proliferation and metastasis of colorectal cancer cells [J]. Journal of Practical Oncology, 2020, 34(5): 418-424.
[5]	SUN Xiaoyan, ZHANG Xiaoyan. Prognostic significance of lncRNA-SChLAP1 high-expression in colorectal cancer [J]. Journal of Practical Oncology, 2020, 34(5): 425-429.
[6]	ZHAO Rencheng, LIN Zitang, YU Weijun, LEI Lin, LIU Zheng, XU Ying, GUO Yanfang, PENG Ji. Analysis of the prevalence of five cancer risks among permanent residents aged 40~74 years old in Baoan District,Shenzhen,2017—2019 [J]. Journal of Practical Oncology, 2020, 34(4): 315-320.
[7]	CHEN Can, ZHANG Yanling, LIU Xiaoqing, RAN Fengwei. The effect of miR-182 on the proliferation and invasion of rectal cancer cells and regulation of Foxo3a/Wnt/β-catenin pathway [J]. Journal of Practical Oncology, 2020, 34(4): 328-333.
[8]	LI Hengzhen, ZHANG Yanqiao. Application progress of liquid biopsy in the diagnosis of colorectal cancer [J]. Journal of Practical Oncology, 2020, 34(4): 357-361.
[9]	PANG Chen, ZHANG Xiuling, ZHANG Xiliu. Research progress in inhibitory epithelium-derived malignant tumors by dendrobium officinale [J]. Journal of Practical Oncology, 2020, 34(4): 362-367.
[10]	ZHU Xiaoyun,ZHANG Yaning,MA Biping. Trend analysis of the death level and the probability of early death caused by colorectal cancer in Jinshan district,Shanghai,1980—2018 [J]. Journal of Practical Oncology, 2020, 34(3): 198-202.
[11]	LUO Lifang,LIU Yunduo,GUAN Yue,CHEN Xiuwei. Quercetin-labeled metal organic framework radiotherapy combined with adriamycin induces apoptosis of cervical cancer HeLa cells [J]. Journal of Practical Oncology, 2020, 34(3): 203-207.
[12]	ZHANG Yao,FAN Dan. The expression and role of LncRNA PSMA3-AS1 in human glioma cells [J]. Journal of Practical Oncology, 2020, 34(3): 214-219.
[13]	LU Xuemei,LI Yuntao,AN Jinhui,JI Guozhong. The mechanism of lysophosphatidic acid in the development of colorectal cancer [J]. Journal of Practical Oncology, 2020, 34(3): 276-281.
[14]	WANG Fei, ZHU Benben. Effects of propofol on the proliferation,apoptosis and invasion of SCC-74 cells via HIF-1 α signal pathway [J]. Journal of Practical Oncology, 2020, 34(2): 120-125.
[15]	LIU Guangxia, ZHAO Lianchun, CHEN Fang, JIA Qian, GAO Wei, HOU Zhan, LU Yamin. Effect and mechanism of ZCCHC12 on malignant transformation of thyroid cells [J]. Journal of Practical Oncology, 2020, 34(2): 133-138.

Salvianolic acid B regulates ROS to inhibit the proliferation and promote apoptosis in colorectal cancer HCT-116 cells

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments