甲基化修饰抑癌基因MSX1抑制黑色素瘤细胞迁移和侵袭的研究

doi:10.11904/j.issn.1002-3070.2020.06.006

Abstract

Abstract: Objective The Objective of this study was to investigate the expression and methylation of MSX1 in melanoma cells and tissues,and its effect on migration/invasion of melanoma A375 cells. Methods qRT-PCR and immunohistochemical were used to examine the expression of MSX1 in A375 cells and tissues;methylation PCR was used to examine the methylation of MSX1 in melanoma tissues;pcDNA3.1(+)-Flag-MSX1(experimental group)and pcDNA3.1(+)-Flag-Vector(control group)were transfected into A375 cells,transwell migration and invasion assay were used to detect the effect of MSX1 on cell migration and invasion;RT-PCR was used to verify the effect of MSX1 on epithelial-mesenchymal transition and its downstream related markers;Westernblot was used to verify the effect of MSX1 on Wnt/β-catenin signaling pathway. Results MSX1 mRNA were down-regulated in human malignant melanoma cell lines and tissues(P＜0.001).The methylation of MSX1 was up-regulated,which was significantly higher than that in adjacent tissues and normal nevus tissues.Overexpression of MSX1 inhibited cell migration and invasion in A375 cells(P＜0.001),while epithelial to mesenchymal transition and the expression of downstream markers were also inhibited.MSX1 overexpressed could inhibit the expression of Wnt/β-catenin signaling pathway and its downstream markers in A375 cells. Conclusion MSX1 was down-regulated in melanoma tissues,indicating that MSX1 inhibits Wnt/β-catenin signaling pathway and participates in the progression of melanoma.

Key words: Melanoma, MSX1, Methylation, Tumor suppressor genes, Migration, Invasion

CLC Number:

R739.5

WANG Can, LUO Qian, LUO Yan, LIU Sutao, YU Yin, DIAO Qingchun, LI Zhi, LI Jing. Inhibitory migration and invasion of melanoma cells by methylation modified tumor suppressor gene MSX1[J]. Journal of Practical Oncology, 2020, 34(6): 511-517.

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URL: http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/10.11904/j.issn.1002-3070.2020.06.006

http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2020/V34/I6/511

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Inhibitory migration and invasion of melanoma cells by methylation modified tumor suppressor gene MSX1

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