miR-106a靶向调控TIMP2对人胃癌细胞增殖、转移及EMT的影响

doi:10.11904/j.issn.1002-3070.2022.02.002

Abstract

Abstract: Objective The aim of this study was to investigate the expression of small RNA-106a(miR-106a)in human gastric cancer tissues and its relationship with cancer cell proliferation and metastasis.Methods A total of 50 pairs of human gastric cancer and paired paracancerous formalin-fixed-embedded samples were collected,and real-time PCR was used to detect the expression of miR-106a in gastric cancer tissues,human low differentiated gastric cancer or poorly differentiated gastric cancer SGC-7901,BGC-823,MKN-45 cell lines and immortalized human gastric mucosal epithelial GES-1 cell line.MTT method was used to detect the cell proliferation,and transwell method was used to detect cell migration and invasion.The bioinformatics analysis and dual luciferase assay were used to identify target genes of miR-106a,and Western blot was used to detect the expression of target proteins TIMP2,MMP2,MMP9,E-cadherin and N-cadherin.Results Real-time PCR result showed that miR-106a was highly expressed in human gastric cancer tissues,and the difference was statistically significant when compared with adjacent tissues(P＜0.001).MiR-106a was also highly expressed in human gastric cancer SGC-7901,BGC-823 and MKN-45 cells,and the difference was statistically significant when compared with GES-1 cells(P＜0.001).MTT assay showed that the proliferation ability of SGC-7901 cells and BGC-823 cells were decreased after inhibiting miR-106a(P＜0.01).Transwell assay showed that the migration and invasion abilities of BGC-823 cells were also decreased(P＜0.001).The dual-luciferase assay showed that after co-transfection of TIMP2 wild type reporter gene and miR-106a mimic,its luciferase activity was significantly lower than that of the miR-106a NC group(P＜0.001),but there was no significant change in the TIMP2 mutant reporter gene.Western blot showed that after inhibiting miR-106a,the expression of TIMP2 was increased,the expressions of MMP2 and MMP9 were decreased,the expression of E-cadherin was increased,and the expression of N-cadherin was decreased.Conclusion The high expression of miR-106a in human gastric cancer may affect the proliferation,metastasis and EMT of gastric cancer cells by targeting TIMP2.

Key words: Gastric tumor, microRNA-106a, Proliferation, Metastasis, Epithelial-mesenchymal transition

CLC Number:

R735.2

ZHANG Ning, LIN Liuya, LI Sifan, ZHU Meng. Effects of miR-106a targeting TIMP2 on proliferation,metastasis and EMT of human gastric cancer[J]. Journal of Practical Oncology, 2022, 36(2): 105-110.

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URL: http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/10.11904/j.issn.1002-3070.2022.02.002

http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2022/V36/I2/105

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Effects of miR-106a targeting TIMP2 on proliferation,metastasis and EMT of human gastric cancer

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