lncRNA CASC2对结直肠癌细胞功能及血管生成的影响

doi:10.11904/j.issn.1002-3070.2022.02.003

Abstract

Abstract: Objective The of this study was to investigate the effect of lncRNA CASC2 overexpression on the proliferation,migration,angiogenesis and Wnt/FZD/β-catenin signaling pathway of colorectal cancer cells.Methods qRT-PCR was used to detect the expression of lncRNA CASC2 in normal colon epithelial cells(NCM460)and colorectal cancer cell lines(DLD-1,HT29,SW620,HCT116,RKO,LOVO,and SW480).HCT116 cells and SW480 cells were established stable transfection.The transfected cell lines were divided into the control group and lncRNA CASC2 overexpression group.CCK-8,Transwell,and scratch assays were used to detect the cell proliferation and migration ability.The cell supernatants collected from the control group and the lncRNA CASC2 overexpression group were used to extract exosomes,which were incubated with HUVEC cells.Their effects on angiogenesis were detected by 3D Matrigel method.The protein expression of Wnt16,FZD7 and β-catenin were determined by Western blot.Results The expression of lncRNA CASC2 in 7 colorectal cancer cell lines was significantly lower than that in normal colon epithelial NCM460 cells.Compared with the control group,the overexpression of lncRNA CASC2 significantly inhibited the proliferation,migration of HCT116 cells and SW480 cells(P＜0.01).3D Matrigel assay showed that exosomes from cells in SW480-CASC2 and HCT116-CASC2 groups significantly inhibited the formation of tubular cells in HUVEC(P＜0.01).The results of Western blot showed that the levels of Wnt16,FZD7 and β-catenin protein decreased after lncRNA CASC2 overexpression(P＜0.01).Conclusion The expression of lncRNA CASC2 is down-regulated in colorectal cancer cells.Overexpression of lncRNA CASC2 can inhibit the proliferation and migration of colorectal cancer cells by inhibiting the Wnt/FZD/β-catenin signaling pathway,and reducing angiogenesis.

Key words: Long non-coding RNA CASC2, Colorectal cancer, Exosomes, Wnt signaling pathway, Angiogenesis

CLC Number:

R735.3

CHEN Han, LUO Xiaoyong, LI Chaoping, GUO Cairu, JU Wencui, ZHAI Yangyang, SHI Xinpeng. The effect of lncRNA CASC2 on the function and angiogenesis of colorectal cancer cells[J]. Journal of Practical Oncology, 2022, 36(2): 111-118.

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http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2022/V36/I2/111

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The effect of lncRNA CASC2 on the function and angiogenesis of colorectal cancer cells

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