CCDC8和TGF-β1在非小细胞肺癌中的表达及临床意义

doi:10.11904/j.issn.1002-3070.2019.03.005

Abstract

Abstract: Objective The aim of this study was to investigate the expression of CCDC8(coiled-coil domain containing 8,P90)and transforming growth factor β 1(TGF- β1)in none small cell lung cancer(NSCLC),and its relationship with clinicopathological characteristics.Methods The expression of CCDC8 and TGF-β1 in NSCLC was analyzed in the TCGA database.The Kaplan-Meier curve was plotted to analyze its correlation with survival of patient.The expression of CCDC8 and TGF-β1 in 204 cases of conventional paraffin-embedded NSCLC was detected by immunohistochemical staining and their relationship with clinicopathological features of NSCLC was analyzed.The relationship between the expression of CCDC8 and TGF-β1 and the overall survival(OS)of patients was analyzed by Cox regression.Results The expression of CCDC8 in NSCLC patients was positively correlated with TGF-β1 expression in the TCGA data(P＜0.05).OS was low in patients with the low expression of CCDC8(HR=1.32,P=0.0437).In 204 NSCLC tissues,CCDC8 expression was positively correlated with TGF-β1 expression(P=0.023).Cox univariate analysis of CCDC8 expression in NSCLC was associated with OS(P=0.013);whereas the expression of CCDC8 and TGF-β1 was not associated with survival in patients with NSCLC(P=0.967,P=0.816).Cox multivariate analyses of TNM staging and CCDC8 were prognosis factors in patients with NSCLC(P=0.016).Conclusion The expression of CCDC8 is correlated the expression of TGF-β1 in NSCLC.CCDC8 may be a prognostic factor for patients with NSCLC.

Key words: CCDC8, TGF-β1, NSCLC

CLC Number:

R734.2

SUN Lichun,LI Dandan,LI Jing,YU Fengling. Expression and clinical significance of CCDC8 and TGF-β1 in non-small cell lung cancer[J]. PRACTICAL ONCOLOGY JOURNAL, 2019, 33(3): 215-221.

References 1

Siegel RL,Miller KD,Jemal A.Cancer Statistics[J].CA Cancer J Clin,2017,67(1):7-30.2 朱晓云,张亚宁,林菲等.上海市金山区2002-2013年肺癌发病及死亡分析[J].实用肿瘤学杂志,2018,32(5):437-441.3 张婷,陶春英,李云芬,等.CCDC8基因的研究现状[J].中国医药指南,2013,11(19):88-90.4 Hanson D,Murray PG,Coulson T,et al.Mutations in CUL7,OBSL1 and CCDC8 in 3-M syndrome lead to disordered growth factor signalling[J].J Mol Endocrinol,2012,49(3):267-275.5 Wei M,Zhao X,Liu M,et al.Inhibition of HIV-1 assembly by coiled-coil domain containing protein 8 in human cells[J].Sci Rep,2015,5:14724-14735.6 Kristen L,Drucker GJ,Kitange TM,et al.Characterization and gene expression profiling in glioma cell lines with deletion of chromosome 19 before and after microcell-mediated restoration of normal human chromosome 19[J].Genes Chromosomes Cancer,2009,48(10):854-864.7 Law JH,Li Y,To K,et al.Molecular decoy to the Y-box binding protein-1 suppresses the growth of breast and prostate cancer cells whilst sparing normal cell viability[J].PLoS One,2010,5(9):e12661.8 Elf S,Blevins D,Jin L,et al.p90RSK2 is essential for FLT3-ITD- but dispensable for BCR-ABL-induced myeloid leukemia[J].Blood,2011,117(25):6885-6894.9 Pangeni RP,Channathodiyil P,Huen DS,et al.The GALNT9,BNC1 and CCDC8 genes are frequently epigenetically dysregulated in breast tumours that metastasise to the brain[J].Clin Epigenetics,2015,7:57-73.10 McLean S,Di Guglielmo GM.TGFβ in endosomal signaling[J].Methods Enzymol,2014,535:39-54.11 Kajdaniuk D,Marek B,Borgiel-Marek H,et al.Transforming growth factor β1(TGFβ1)in physiology and pathology[J].Endokrynol Pol,2013,64(5):384-396.12 Watanabe Y,Itoh S,Goto T,et al.TMEPAI,a transmembrane TGF-beta-inducible protein,sequesters Smad proteins from active participation in TGF-beta signaling[J].Mol Cell,2010,37(1):123-134.13 Imai K,Minamiya Y,Goto A,et al.Bronchioloalveolar invasion in nonsmall cell lung cancer is associated with expression of transforming growth factor-β1[J].World J Surg Oncol,2013,11:113-117.14 李云芬,聂建云,张婷,等.CCDC8基因在乳腺癌中的表达[J].肿瘤研究与临床,2014,26(4):226-229.15 李云芬,唐一吟,王建逵,等.CCDC8基因表达与乳腺癌分子分型的相关性研究[J].昆明医科大学学报,2014,35(12):24-28.16 Morris MR,Ricketts CJ,Gentle D,et al.Genome-wide methylation analysis identifies epigenetically inactivated candidate tumour suppressor genes in renal cell carcinoma[J].Oncogene,2011,30(12):1390-1401.17 Jiang GY,Zhang XP,Zhang Y,et al.Coiled-coil domain-containing protein 8 inhibits the invasiveness and migration of non-small cell lung cancer cells[J].Hum Pathol,2016,56:64-73.18 Yang CK,Yu TD,Han CY,et al.Genome-wide association study of MKI67 expression and its clinical implications in HBV-related hepatocellular carcinoma in southern china[J].Cell Physiol Biochem,2017,42(4):1342-1357.19 董凤萍.CCDC8基因对乳腺癌细胞株MDA-MB-231的作用及与ANKRA2、LKB1、14-3-3σ之间相互关系的研究[D].昆明:昆明医科大学,2016.20 汤晗.CCDC8基因沉默对裸鼠乳腺癌移植瘤生长影响的研究[D].昆明:昆明医科大学,2017.

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Expression and clinical significance of CCDC8 and TGF-β1 in non-small cell lung cancer

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