长链非编码RNA PSMA3-AS1在人胶质瘤细胞中的表达及作用研究

doi:10.11904/j.issn.1002-3070.2020.03.005

Abstract

Abstract: Objective The aim of this study was to detect the expression of lncRNA PSMA3-AS1 in glioma tissues and cell lines(U251、LN229、T98G and A172)and its effect on glioma cells.Methods Quantitative qRT-PCR method was used to evaluate the expression of PSMA3-AS1 in 35 glioma tissues;Combined with TCGA databases,the correlation between PSMA3-AS1 expression and the degree of malignancy and the prognosis of glioma patients was analyzed;Cell proliferation and invasion were explored in U251 and T98G glioma cells;The effects of PSMA3-AS1 on invasion related proteins MMP-2/9 in glioma cells were detected by Western blot assay.Results TCGA database indicated that PSMA3-AS1 was increased in glioma tissues,and the expression of PSMA3-AS1 in 35 paired glioma tissues and glioma cells was significantly increased than in adjacent normal brain tissues(P＜0.05).The expression of PSMA3-AS1 was correlated with tumor mean diameter(P=0.007),and high PSMA3-AS1 exhibited poorer overall survival in glioma patients(P=0.042).After knockdown the level of PSMA3-AS1,the proliferation and invasion abilities of glioma cells were significantly inhibited and the expression of MMP-2/MMP9 were decreased in glioma cells(P＜0.05).Conclusion The level of PSMA3-AS1 is significantly higher in glioma tissues with a role of promoting cancer in glioma and may be a potential therapeutic target.

Key words: lncRNA, Glioma, Cell proliferation, Invasion

CLC Number:

R739.41

ZHANG Yao,FAN Dan. The expression and role of LncRNA PSMA3-AS1 in human glioma cells[J]. Journal of Practical Oncology, 2020, 34(3): 214-219.

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URL: http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/10.11904/j.issn.1002-3070.2020.03.005

http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2020/V34/I3/214

References

1 Jalbert LE,Elkhaled A,Phillips JJ,et al.Metabolic profiling of IDH mutation and malignant progression in infiltrating glioma[J].Sci Rep,2017,7:44792.
2 Galldiks N,Kocher M,Langen KJ.Pseudoprogression after glioma therapy:an update[J].Expert Rev Neurother,2017,17(11):1109-1115.
3 Li C,Zheng H,Hou W,et al.Long non-coding RNA linc00645 promotes TGF-β-induced epithelial-mesenchymal transition by regulating miR-205-3p-ZEB1 axis in glioma[J].Cell Death Dis,2019,10(10):717.
4 Kopp F,Mendell JT.Functional classification and experimental dissection of long noncoding RNAs[J].Cell,2018,172(3):393-407.
5 Cui H,Zhang Y,Zhang Q,et al.A comprehensive genome-wide analysis of long noncoding RNA expression profile in hepatocellular carcinoma[J].Cancer Medicine,2017,6(12):2932-2941.
6 Qiu BQ,Lin XH,Ye XD,et al.Long non-coding RNA PSMA3-AS1 promotes malignant phenotypes of esophageal cancer by modulating the miR-101/EZH2 axis as a ceRNA[J].Aging(Albany NY),2020,12(2):1843-1856.
7 Capdevila C,Rodríguez Vázquez L,Martí J.Glioblastoma multiforme and adult neurogenesis in the ventricular-subventricular zone:a review[J].J Cell Physiol,2017,232(7):1596-1601.
8 Zhou S,Wang L,Yang Q,et al.Systematical analysis of lncRNA-mRNA competing endogenous RNA network in breast cancer subtypes[J].Breast Cancer Res Treat,2018,169(2):267-275.
9 Zhao Y,Wang H,Wu C,et al.Construction and investigation of lncRNA-associated ceRNA regulatory network in papillary thyroid cancer[J].Oncol Rep,2018,39(3):1197-1206.
10 Li J,Meng Z,Gang A,et al.LncRNA TUG1 acts as a tumor suppressor in human glioma by promoting cell apoptosis[J].Exp Biol Med,2016,241(6):644-649.
11 He Z,Wang Y,Huang G,et al.The lncRNA UCA1 interacts with miR-182 to modulate glioma proliferation and migration by targeting iASPP[J].Arch Biochem Biophys,2017,623/624:1-8.
12 Lu Y,Li Y,Chai X,et al.Long noncoding RNA HULC promotes cell proliferation by regulating PI3K/AKT signaling pathway in chronic myeloid leukemia[J].Gene,2017,607:41-46.
13 范宁宁,耿敬姝.长链非编码RNA MEG3在人胶质瘤细胞中的表达及作用研究[J].实用肿瘤学杂志,2018,32(3):219-223.
14 Xu H,Han H,Song S,et al.Exosome-transmitted PSMA3 and PSMA3-AS1 promote proteasome inhibitor resistance in multiple myeloma[J].Clin Cancer Res,2019,25(6):1923-1935.
15 He Y,Meng XM,Huang C,et al.Long noncoding RNAs:novel insights into hepatocelluar carcinoma[J].Cancer Lett,2014,344(1):20-27.
16 李思维,庞达.长链非编码RNA(lncRNA)在癌症中的作用[J].实用肿瘤学杂志,2019,33(5):471-475.
17 Zhao XY,Li S,Wang GX,et al.A long noncoding RNA transcriptional regulatory circuit drives thermogenic adipocyte differentiation[J].Mole Cell,2014,55(3):372-382.
18 Xia T,Liao Q,Jiang X,et al.Long noncoding RNA associated-competing endogenous RNAs in gastric cancer[J].Sci Rep,2014,4:6088.

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The expression and role of LncRNA PSMA3-AS1 in human glioma cells

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