Abstract: In recent years,the isotope-labeled relative and absolute quantification(Isobaric tags for relative and absolute quantification,iTRAQ)technology has become a powerful tool for the quantitative research of proteomics,especially in tumor-related fields.The advantages are a wide range of protein determination,reliable analytical results,high accuracy and good repeatability.A large number of literatures have also shown that iTRAQ technology has been widely used in gastrointestinal tumors,such as esophageal cancer,gastric cancer,liver cancer and other tumor marker screening research,the mechanism of tumor development,and the exploration of tumor treatment and prognosis.In the era of large-scale quantitative biology,iTRAQ technology is increasingly indispensable in the molecular level of in-depth understanding of the relevant mechanisms of digestive tract tumors.Therefore,this article briefly reviews the research progress of iTRAQ technology in gastrointestinal tumors.

Key words: iTRAQ, Gastrointestinal tumors, Proteomics