Abstract: In recent years, the proteomic technology and theory have been more and more widely used in bioscience and medical research with its increased maturity and perfection.The research about blood, gastric fluid, tissues, cells and cell organelles can completely explain the rules of life and provide evidences for further research.The detection for occurrence, developing and metastasis-related protein in gastric cancer is benefit for discovering novel specificity markers or understanding the pathogenesis mechanism, and is of great importance for early diagnosis and target therapy.