熊果酸调节Wnt/β-catenin信号通路对甲状腺癌细胞增殖、凋亡、侵袭和迁移的影响

doi:10.11904/j.issn.1002-3070.2026.01.004

Abstract

Abstract: Objective This study aimed to investigate whether ursolic acid(UA)could inhibit the malignant biological behaviors of thyroid cancer cells by regulating the Wnt/β-catenin pathway. Methods Human thyroid cancer SW579 cells were divided into four groups:the control group(treated with DMSO as a blank control),the low-dose UA group(2.5 μmol/L UA),the high-dose UA group(5 μmol/L UA),and the high-dose UA+Wnt agonist group(5 μmol/L UA + 0.3 μmol/L Compound 3f).The CCK-8 assay was used to detect the effect of UA(0-10 μmol/L)on the proliferation of SW579 cells;The colony formation assay was performed to evaluate the proliferative capacity of SW579 cells;Flow cytometry was used to detect apoptosis of SW579 cells;The cell scratch assay was conducted to assess migration ability of SW579 cells;The Transwell assay was performed to determine invasive capacity of SW579 cells;Western blot was employed to detect the protein expression of Wnt1,β-catenin,B-cell leukemia/lymphoma 2(BCL2),and BCL2-associated X protein(Bax)in SW579 cells. Results Compared with the 0 μmol/L group,UA treatment gradually enhanced the inhibitory effect on the proliferation of SW579 cells with increasing concentration(P＜0.05),and the half maximal inhibitory concentration(IC₅₀)for UA to inhibit SW579 cell proliferation was 5.22 μmol/L.Compared with the control group,UA in the low-dose and high-dose UA groups significantly reduced the colony formation rate,scratch healing rate,and number of invasive cells as well as decreased the protein expression of BCL2,Wnt1 and β-catenin in SW579 cells,while the apoptotic rate and the protein expression of Bax were significantly increased(P＜0.05).Compared with the high-dose UA group,the high-dose UA + Wnt agonist group exhibited significantly elevated the colony formation rate,scratch healing rate,number of invasive cells,and increased the protein expression of BCL2,Wnt1 and β-catenin,whereas the apoptotic rate and Bax protein expression were significantly decreased(P＜0.05). Conclusion UA may regulate the Wnt/β-catenin pathway by down-regulating the expression of Wnt1 and β-catenin,thereby inhibiting the proliferation,migration and invasion of human thyroid cancer cells and inducing apoptosis.

Key words: ursolic acid, Wnt/β-catenin pathway, thyroid cancer, proliferation, apoptosis, migration, invasion

CLC Number:

R285.5

XU Jing, GUO Zhuying, HE Miao, ZHOU Wenhui, LIU Bin. The effects of ursolic acid on the proliferation,apoptosis,invasion,and migration of thyroid cancer cells by regulating the Wnt/β-catenin pathway[J]. Journal of Practical Oncology, 2026, 40(1): 22-29.

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URL: http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/10.11904/j.issn.1002-3070.2026.01.004

http://journal09.magtechjournal.com/Jwk3_syzlxzz/EN/Y2026/V40/I1/22

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The effects of ursolic acid on the proliferation,apoptosis,invasion,and migration of thyroid cancer cells by regulating the Wnt/β-catenin pathway

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