Objective To investigate the effect of Survivin gene on the growth of human colorectal cancer HT-29 cells and to explore the feasibility of gene therapy in human colorectal carcinoma.Methods The human colorectal cancer HT-29 cell line was treated with ASODNs.The effects of Survivin-ASODN on the growth of HT-29 were studied by cell colony assay, reverse transcription polymerse chain reaction.The invasive ability was measured by number of cells to penetrate polycarbonates coated with matrigel.Results The number of HT-29 cells colony was inhibited after the cells were treated for 48 hours.The cell proliferation was inhibited effectively, too.The expression of Survivin mRNA decreased sharply in HT-29 cells treated with ASODNs.The cells number that penetrated polycarbonates was significantly lower in the ASODNs group than that of control group.Conclusions The antisense oligodeoxynucleotides were capable of inhibiting the proliferation and the invasive ability of human colorectal carcinoma HT-29 cells.

Objective The aim of this study is to summarize and discuss the Results of video-assisted thoracoscopic surgery(VATS)in 58 patients with thoracic tumors.Methods From July 2005 to June 2007, 58 patients with thoracic tumors underwent VATS for peripheral pulmonary nodules, leiomyoma of esophagus, mediastinal lymphadenectasis, malignant pleumal fluid, bronchogenic cyst, medi-astinal cyst, neurofibroma.Results All procedures were performed successfully under VATS except for 3 cases, who were converted to thoracotomy for lung cancer.There was no mortality or severe complication in this series.Conclusion VATS is a safe and effective technique in selected patients with thoracic lesions.The overall incidence of perioperative complication is low.VATS has obvious advantages in treatment of benign thoracic lesions, however, the indications should be selected carefully for malignant tumors.

Objective To study clinicopathological feature of anorectal malignant melanoma and the role of immunohistochemical stain in diagnosis.Method 10 cases of anorectal malignant malanoma were studied.Expression of HMB45, S-100, Vimetin were detected by immunohistochemistry with S-P method.Result 1 case is malignant melanoma.9 cases were misdiagnosed as other diseases.Clinicopathologic features:7 cases with essential epitheliod cell, 2 cases with mainly spindle cell, 1 case with small lymphocyte-like cell.All 10cases of anorectal malignant melanoma were positive to HMB45, S-100 and 9 cases were positive to vimetin and 1 case were positive to CK, but negative to LCA.Conclusion The faeces with fresh blood, no evident mucus.The masses were nodular, fungiform or ulcerative.Histopathologic observation supported the idea that this tumor developed from the melanocytes.10 cases at the basal-layer of the epithelium.Immunohistochemistry proved that melanocytes originated from the neural crest.Immunohistochemical stain HMB45, Vimetin S-100 were helpful for diagnosis of malignant melanoma.

Objective To observe the therapeutic efficacy of chemotherapy and the concurrent chemo-radiotherapy in treatment of extensive stage small cell lung cancer(ES-SCLC). Methods We retrospectively analyzed 68 patients with ES-SCLC.Among them, 40 patients received chemotherapy(CT)and concurrent local radiotherapy at the end of the fourth CT cycle;the other 28 patients only received chemotherapy.Both groups used the EP-CAV regimen for six cycles.Results The short-term therapeutic effect:responsive rates of combined group and CT group are 82.5% and 57.2%, respectively(P＜0.05).The long-term therapeutic effct:the one-year survival rates are 52.5% for combined group and 28.6% for the CT group( P＜0.05).The two-year survival rates are 20% and 7.14%, respectively(P＞0.05).The median-survival time are 13 months in combined group and 8 months in CT group.Conclusions The short term therapeutic effect and one-year survival rate of the combined group are better than that of CT group(P＜0.05), the differences are significant.The difference of two-year survival rate is not significant, but the combined group showed better survival tendency.

Objective To study the therapeutic effect and side effects of combined therapy with paclitaxel liposome and Nedaplatin on advanced ovary cancer.Methods 80 cases of ovary cancer with advanced stage were studied.A combined chemotherapy of paclitaxel liposome and Nedaplatin were applied.The dose of paclitaxel liposome was 135mg/m² with Nedaplatin 80mg/m²-100mg/m².The total course of treatment were three weeks, all cases finish at least 1-2 course of treatment. Results The bone marrow depression incidence was 50%, Gastrointestinal tract reaction rate was 77.5% No patient exit the therapy because of side effects.Conclusions The combined therapy of paclitaxel liposome and NDP showed good efficacy accompanied by less side effects.

Objective To study the significance of MMP-3 and p53 expression in patients with mammary carcinoma. Methods The expression of MMP-3 and p53 were detected by immunohistochemical S-P in 58 mammary infiltration duct carcinoma tissue samples and their clinical data were statistically analysed. Results In 58 mammary carcinoma tissue samples, the expression of MMP-3 was 44.83% and the expression of p53 was 37.93%.The expression of MMP-3 was correlated significantly with lymph node metastasis, P＜0.05;The expression of p53 was correlated significantly with the size of cancer, P＜0.05. Conclusions The expression of MMP-3, p53 can be taken as the biological marker of prognosis and postoperative comprehensive treatment of breast cancer.

Objective Colorectal cancer in China has high morbidity and lethality and the epidemiologic characters such as increasing incidence, changing constituent ratio and clinical features.This study was to explore the clinical characteristics between right colon cancer and rectal cancer in Heilongjiang region among 15 years.Methods The clinical data of 2552 cases were confirmed pathologically and divided into 5-year groups were analyzed retrospectively.Result Compared with 1992-1997 group, right colon cancer and rectal cancer in the 2002-2006 had the following characters:the annual increase ratio were 6.7% and 6.0% respectively in hospitalization number;the median age of incidence increased from 53.1 and 52.8 to 60.5 and 57.6 respectively;the male/femal ratio decreased from 1.38∶1 to 1.19∶1 in right colon cancer and no significant variation was found in rectal cancer;the constituent ratio of rectal cancer decreased from 67.4% to 61.8% and right colon cancer no change;the proportion of Dukes A stage increased 3.8% and 2.6%, while Dukes D stage decreased 2.8% and 3.7% respectively.Conclusion In the past 15 years, the incidence of colorectal cancer were increasing in Heilongjiang province with an increase of median age of incidence;the constituent ratio of colorectal cancer were decreasing in rectal cancer and no change in right colon cancer, while Dukes A were increasing and Dukes D decreasing.