Objective Our study aims to investigate the inhibition effects of miR-124 on the growth of breast cancer cell MCF-7 induced by paclitaxel. Methods MTT was used to detect the growth inhibition of MCF-7 induced by paclitaxel. Flow cytometry was used to detect the effect of paclitaxel on cell cycle. Real-time quantitative PCR(qRT-PCR) was used to detect the expression level of miR-124, while MCF-7 celss were treated with paclitaxel. MiR-124 inhibitor was transfected into MCF-7 breast cancer cells, and growth inhibition was detected by MTT. Results The results showed that paclitaxel could significantly inhibit the growth of breast cancer cell line MCF-7 by blocking the G2 phase. The results from qRT-PCR showed that the relative expression of miR-124 was increased when the dosage of paclitaxel was increased. When the expression of miR-124 was inhibited, the cell growth inhibition caused by paclitaxel was also prominent decreased. Conclusion The higher expression of miR-124 in MCF-7 induced by paclitaxel was dose dependent. And miR-124 inhibitor can significant influence the cell growth inhibition caused by paclitaxel. These results indicated that miR-124 plays an important role in paclitaxel-induced chemotherapy drug resistance, and provides a new direction to solve the problem.

Objective To investigate the expression of ERCC1 and β-Tubulin-?Ⅲ in ovarian epithelial carcinoma and their correlation with pathological characteristics as well as clinical significance, thus to provide theoretical basis for ovarian epithelial carcinoma individualized treatment. Methods The mRNA expressions of ERCC1 and TUBB3 in ovarian epithelial cancer tissues were detected by reverse transcription real-time fluorescence quantitative reactin (RTq-PCR).We analyzed the correlation of the expression of ERCC1 mRNA and TUBB3 mRNA and clinical pathological features of patients. Results ERCC1 and TUBB3 positively expressed in epithelial ovarian cancer. The rate of high expression of ERCC1 was 28.21%（22/78）and that of TUBB3 high expression was 29.49%（23/78）. By univariate analysis, high expression of ERCC1 mRNA and TUBB3 mRNA was only correlated with the size of residual mass（P＜0.05）, but not other clinical and pathological features. ERCC1 and TUBB3 expression was moderately correlated with each other（r=0.4249，P<0.05）. Conclusion ERCC1 and TUBB3 positively expresses in epithelial ovarian cancer and they are correlated with each other. High expression of ERCC1 mRNA and TUBB3 mRNA is positively correlated with the size of residual mass.

Objective The purpose of this study is to study the expression of miR-221 in cervical cancer tissues and its relationship with HPV infection. Methods HR-HPV infection was detected by HC2, and 30 cases of HR-HPV negative and 5 cases of HR-HPV positive cervical cancer tissues were collected. Meanwhile, 30 cases of normal cervical tissues in patients with benign disease were collected as control group. The expression of miR-221 was detected by RT-PCR, preliminarily investigating the relationship between miR-221 expression and the occurrence of cervical cancer and HPV infection. Through transfection of miR-221 and anti-miR-221 into HPV16-positive cervical carcinoma cell line Caski and HPV16-negative cervical carcinoma cell line C33a, we observed the role of miR-221 on the migration and invasion of Caski cells and C33a cells. Results Compared with normal cervical tissues, the expression of miR-221 in cervical cancer was significantly increased, the difference was statistically significant (P<0.01); and the expression of miR-221 is closely correlative to patients with or without lymph node metastasis, pathological grade and clinical stage (P<0.01); the expression of miR-221 in HR-HPV positive cervical cancer tissues was higher than in HR-HPV negative cervical cancer tissues (P<0.01); transfection of miR-221 and anti-miR-221 could promote or downregulate C33a and Caski cells migration and invasion, and the changes between two groups had statistical significance (P<0.05). Conclusion The increased expression of miR-221 in cervical cancer tissues is closely related to the occurrence and development of cervical cancer and HPV infection.

ObjectiveTo observe the effect of Galectin-3 gene knock out to the mouse skin tumor growth and discuss the mechanism of inhibition of the mouse cutaneous tumor induced by TPA which caused by Galectin-3 knock out. MethodsThe DMBA + TPA multi-step induced skin tumor in mice model were used to establish the skin cancer model. The control group was the same age wild type mice. We observed the inhibition of the mouse tumor growth by Galectin-3 knock out. In situ tumor cells were collected and cultured on soft agar for colony formation assay. The side population of the situ cancer cells was analyzed quantitatively by flow cytometry. Results1. Compared with wild type mice group (group A), Galectin-3 knock out mice group (group B) displayed a significant delay of the appearance of tumor. The tumor incidence and the average number of tumor per mice between group A and group B had obvious difference (P < 0.01). 2 . In vitro, data of soft agar colony formation assay showed that colony formation rate in group A are significantly higher than group B (P < 0.01). 3. The collection and separation of A and B group in situ tumor cells, using flow cytometry instrument for in situ tumor cell side population quantitative analysis, group A compared with group B had obvious difference (P < 0.01). ConclusionThe knock out of the Galectin-3 gene reduces the skin cancer stem cells, inhibits tumor cell proliferation, depresses chemical carcinogenesis of mice skin. Galectin-3 gene may become the new target for skin cancer chemotherapy.

ObjectiveTo study effects of signal transducer and activator of transcription 3 (STAT3) gene on lung adenocarcinoma cells EMT. Methods We observed morphological changes of the cells and detected the expression of STAT3 and Twist by western blot after STAT3-SiRNA plasmid was transfected into A549 cells. Meanwhile, we observed invasion and migration ability of A549 cells using transwell method and wound healing assay. Eventually, we verified the correlation between STAT3 and Twist expression in lung adenocarcinoma tissues by immunohistochemical method. Results Si-STAT3 significantly down-regulated the expression of STAT3 in A549 cells, and pronouncedly inhibited migration and invasion of A549 cells in vitro. Meanwhile, we also found decreased expression of Twist. The expression of STAT3 was positively correlated with the expression of Twist in lung adenocarcinoma tissue specimens and the expression of two genes was reversely correlated with tissue differentiation degree. Conclusion The STAT3 participated in the process of EMT by regulating twist expression, which played a vital role in progression of lung adenocarcinoma.

Objective To detect the expression of CD44+/CD24- and E-cadherin and vimentin in non-small cell lung cancer and its cancer prevention and treatment targets. cancer stem cells , which can lead to the development of malignant cells poorly differentiated trends，they are not an isolated process ; important role in the development of EMT and CD44+/CD24- union occurs in non-small cell lung cancer and prognosis with patients , which provides a potential breakthrough in the treatment of

Objective Two-incision video-assisted thoracic surger relieved post operative pain when compared with open thoractomy, while it is rarely reported worldwide，most thoracic surgeons think it is hard to finish the complicated operation and it is not safe.We compared the safety between open and two-incision VATS. Methods Bwteen Febrary 2009 to December 2011, a total of 334 cases with clinical early-staged lung cancer of open thoracotomy were performed , 66 cases were completely performed with 2-incision VATS, 17 cases were transferred to open thoracotomy defined as two-incision VATS assisted thoracotomy. We compared and analyzed open thoracotomy with two-incision VATS in operating time, and pre,post and total period of hospitalization, postoperative chest tube removal time, postoperative complications. Results Operating time in the left lower lobe of both traditional open thoracotomy and two-incision VATS was 162.5 ± 6.5 and 185.8 ± 12.8 minutes respectively(p=0.1228),there was no statistical significance for the remaining parts of the lobectomy, the operating time of open thoracotomy was shorter than two-incision VATS. The overall complication and perioperative mortality rate of open thoracotomy and two-incision VATS were 10.2% and 15.0%(P = 0.238), and 2.0% and 0.0% (p=1.000)respectively, there was no statistical significance. ConclusionThe lobectomy and lymph node dissections for 2-incision VATS in treating clinical stage I lung cancer is feasible and safe.

Objective Hyalinizing trabecular tumor (HTT) is often mistaken as thyroid papillary carcinoma(TPC), which shares some morphological features with TPC. The aim of this study is to investigate HTT and TPC with immunohistochemical methods. Methodswe detected the expression of the three Immunohistochemical index((CK19, HBME-1,MIB-1) in thirteen cases HTT and twenty cases TPC. ResultsIn HTT samples, CK19: three of the thirteen were positive and focal positivity（1+）; HBME-1: None of the thirteen samples was stained. MIB-1: ten in thirteen cases were stained in nucleus. In TPC samples, CK19: all of the twenty samples were intensely stained; HBME-1: nineteen of twenty samples were intensely stained; MIB-1: all of the twenty samples were stained in nucleus. The sensitivity and specific degrees of CK19 and HBME-1 combination to diagnosis HTT and TPC were 90.0% and 69.2%. Conclusion Our research could provide potent aid to differential diagnosis of HTT and TPC. The combination of CK19 and HBME-1 are adequate to identify HTT and TPC.

Objective To detect the TNFSF13 expression and to evaluate its possible use as a potential prognostic marker in breast cancer. Methods We analyzed TNFSF13 expression by Western blot and qRT-PCR in 93 breast cancer patients. Results Our results showed that TNFSF13 was overexpressed in breast cancer tissue.High TNFSF13 mRNA expression was closely correlated with tumor differentiation, pN classification（P = 0.003，0.022）. In addition, patients with high TNFSF13 expression had a significantly shorter survival analyzed by Kaplan-Meier (P=0.04, log-rank test). Cox proportional hazards regression multivariable analysis revealed that high expression of TNFSF13 was identified as an independent prognostic factor (P=0.03). Conclusion TNFSF13 expression might be a novel target for prognosis and intensive therapy in breast cancer patients.

ObjectiveTo investigate the biological characteristics,therapy selection of tubular carcinoma of the breast. Relationship between axillary lymph node metastasis and other factors were analyzed retrospectively. MethodsTubular carcinoma patients were collected in our hospital from June, 1987 to March, 2014. Paraffin tissues were re-checked again by pathology doctors and all patients were performed follow-up by telephone to evaluate the prognosis. Results68 cases of tubular carcinoma of the breast were chosen. Axillary lymph node metastasis is closely related to tumor size and tumor grade in our research. In all patients, only one patient was relapsed, 24 patients died of other diseases, without distant metastasis or death of breast cancer. ConclusionTubular carcinoma of the breast has a good prognosis with low recurrence rate. The treatment of tubular carcinoma is not quite reasonable. Preoperative core needle biopsy and surgery axillary staging should be paid more attention and breast conserving rate is still needed for improvement.

AbstractMesenchymal chondrosarcoma originated in the primitive mesenchymal tissue. It usually develops in the short bones such as hand, foot and body bone, while extremeIy rare in the orbit．We report a case of mesenchymal chondrosarcoma of the orbit which is confirmed by pathology .

Abstract Monoclonal antibodies (mAbs) to human TRAIL receptors (TRAIL-Rs) have been developed in tumor targeted therapy and currently used in clinical Ⅰ-Ⅳ trials . MAbs can bind death-receptors（TRAIL-R1 and TRAIL-R2）and then results in formation of the death inducing signaling complex (DISC) and apoptosis of tumor cells. TRAIL-Rs mAbs have become one of the research and development hotspots of anti-tumor drugs . These mAbs have achieved remarkable results in combination with radio- or chemo-therapy drugs or other agents and immunotherapy in the treatment of tumor and offer a new therapy strategy for clinical tumor treatment.

AbstractThis paper summarizes the progress on anti-tumor effect and its mechanism of Noni over the past decade. Plenty of tests indicated that noni juice, juice extracts, leaf and root extracts have anti-tumor effects. It can inhibit tumor growth through inducing tumor cell apoptosis, activating host immune system, inhibition of angiogenesis and cyclooxygenase, anti-oxidation and blocking carcinogen -DNA adduct formation. This paper provides a reference for the adjuvant therapy of anticancer drugs and food form Noni.

AbstractThe MLN64 gene, which is localized in q12-q21 of the human chromosome 17, encodes a novel transmembrane protein containing 445 amino acids. The C-terminus of MLN64 shares significant homology with the steroidogenic acute regulatory (StAR) protein, while its N-terminal domain exhibits a function of targeting the protein to late endosomes. MLN64 is likely to be involved in cholesterol transport and synthesis of steroid hormones. MLN64 gene, coamplified with C-erbB-2, is overexpressed in certain breast carcinomas and exerts an influence on biological characteristics of breast cancer cells. The high levels of MLN64 observed in some breast carcinomas could contribute to the growth and progression of these tumors through increased intratumoral steroidogenesis, and is considered as a predictive factor of breast cancer prognosis.

Abstract HER2-targeting therapy is an important part of the treatment for breast cancer with HER2 overexpression. However, the therapy effectiveness is largely influenced by HER2 resistance. Previous studies have confirmed that PI3K/AKT/mTOR signaling pathway is activated during the process of drug resistance to HER2-targeting therapy. Therefore, investigating the PI3K/AKT/mTOR signaling pathway and drugs-targeted pathway is important for the treatment of breast cancer.

Abstract Osteosarcoma is a common malignant bone tumor in the skeletal system of minors. The five-year survival rate of patients with osteosarcoma is significanty improved by neoadjuvant chemotherapy combined with surgery. However, its mortality and morbidity rates remain quite high. With the development of molecular biology and genetics, gene therapy provides a new hope for patients with osteosarcoma. Researchers at home and abroad are actively exploring effective therapeutic targets. In this paper, new progress in the study of gene-targeted therapy for osteosarcoma is reviewed.

Abstract Cervical carcinoma is a serious threat to the health of women around the world, and its incidence ranks at the second after breast cancer in female reproductive system. In addition to oncogene activation and inactivation of tumor suppressor gene, endogenous factors and exogenous factors also affect the development of cervical cancer.

Abstract Alteration of metabolism and epigenetic status are two new focus in the field of cancer research. Recently, more and more reports have demonstrated that the changes of both metabolic enzymes and altered epigenetic status have close interrelationship with the pathogenesis and development of gliomas in human. We discuss here the effects of altered metabolic enzymes on epigenetic status in gliomas based on two important altered metabolic enzymes, with the aim to unlock the molecular mechanism of how gliomas occur and progress. This newfound molecular mechanism of how altered metabolic enzymes impact on epigenetic status in gliomas may contribute to the identification of novel therapeutic targets to gliomas in the future.

AbstractChemotherapy is one of the three most common treatment modalities for cancer. However, chemotherapy as current firstline therapy induces significant side effects and limited efficacy, leading to multidrug resistance and fast recurrence challenging the patient survival rate. Drug metabolizing enzymes (DMEs) and efflux transporters promote the metabolism, clearance, and detoxification of chemotherapeutic agents. Nuclear receptors, especially pregnane X receptor (PXR, NR112) and constitutive androstane activated receptor (CAR, NR113), regulate the expressions of target genes that could encode phase I DMEs, phase II DMEs, and efflux transporters in the development of multidrug resistance (MDR) during chemotherapy. Recent studies have revealed that PXR and CAR play pivotal roles in MDR of various human carcinomas. And their expression levels or activation statuses could predict the risk of drug resistance in patients subjected to chemotherapy. Accordingly, PXR/CAR antagonists, combining with existing chemotherapeutics that activate PXR/CAR, are promising options that could overcome MDR in cancer.

AbstractCurrently, EphB receptor has been found that it is an important impact on the biological behavior of normal cells. In some studies, which plays an important role in the regulation of normal cell morphology, cell adhesion, migration and angiogenesis. Another, there is a certain relationship between abnormal expression of EphB receptor with a variety of tumor development. Visible, EphB receptor is important research direction in cancer studies, accurate grasp of the relationship between EphB receptor and rumor can better understand the progress of the tumor, and the means to provide more scientific basis for cancer treatment and prevention.