肺癌患者外周血中MDR-1及MRP基因在化疗前后的表达及意义

doi:10.11904/j.issn.1002-3070.2015.02.007

实用肿瘤学杂志 ›› 2015, Vol. 29 ›› Issue (2): 127-132.doi: 10.11904/j.issn.1002-3070.2015.02.007

肺癌患者外周血中MDR-1及MRP基因在化疗前后的表达及意义

孔庆暖¹,傅兴宁²,丁晓燕³,纪霞⁴,黄维清¹

1.青岛市市立医院病理科(青岛 266071);
2.潍坊市益都中心医院病理科;
3.青岛大学医学院附属医院病理科;
4.青岛市市立医院呼吸内科

收稿日期:2014-10-31 出版日期:2015-04-28 发布日期:2015-05-05
通讯作者: 黄维清,E-mail:huangweiqing_40@163.com E-mail:huangweiqing_40@163.com
作者简介:孔庆暖,女,(1980-),硕士,主治医师,从事肿瘤病理的研究
基金资助:
青岛市公共领域科技支撑计划项目(11-2-3-2-(3)-nsh)

Comparative expression of MDR-1 and MRP in peripheral blood lymphocytes in lung cancer patients with chemotherapy treatment

KONG Qingnuan¹,FU Xingning²,DING Xiaoyan³,JI Xia⁴,HUANG Weiqing¹

1.Department of Pathology,Qingdao Municipal Hospital,Qingdao 266071,China;
2.Department of Pathology,Weifang Yidu Central Hospital;
3.Department of Pathology,The Medical College of Qingdao University Affiliated Hospital;
4.Department of Respiratory Medicine,Qingdao Municipal Hospital

Received:2014-10-31 Online:2015-04-28 Published:2015-05-05

摘要/Abstract

摘要： 目的探讨肺癌患者化疗前后外周血淋巴细胞中多药耐药基因(MDR-1)和多药耐药相关蛋白(MRP)的表达及意义。方法分别于化疗开始前及化疗周期结束后第3周收集肺癌患者外周血标本。采用逆转录聚合酶链反应(RT-PCR)法检测39例肺癌患者外周血中MDR-1mRNA和MRP mRNA的表达情况,并与20例健康体检者进行对比。结果病例组化疗前MDR-1mRNA和MRP mRNA的阳性表达率与健康对照组相比差异没有统计学意义(P＞0.05);各种病理类型的肺癌患者外周血MDR-1mRNA和MRP mRNA的阳性表达水平随着化疗次数的增多而增强,其中小细胞肺癌患者化疗后MDR-1 mRNA和MRP mRNA的表达水平均高于化疗前,差异具有统计学意义。结论化疗可诱导肺癌患者外周血淋巴细胞MDR-1mRNA和MRP mRNA的表达水平增强;应用外周血评估MDR-1mRNA和MRP mRNA的方法简单可靠,创伤性小,可能成为预估肺癌患者化疗疗效的有效指标。

Abstract: Objective To determine the significance of MDR-1 and MRP mRNA expression in peripheral blood lymphocytes in lung cancer patients with chemotherapy treatment.Methods Peripheral blood samples were taken from each lung cancer patient before chemotherapy and three weeks after the first chemotherapy cycle.The expressions of MDR-1 and MRP were tested for 39 lung cancer cases using RT-PCR technique comparing to 20 normal control cases.Results There was no significant difference for MDR-1 mRNA and MRP mRNA existing in lung cancer cases and control group(P＞0.05).MDR-1 and MRP mRNA expressions were increased after treatment of chemotherapy courses in almost all pathological types of lung cancer.Furthermore,the expression level in small cell lung cancer after chemotherapy was significantly higher than that before.Conclusion Chemotherapy may induce the incidence rate of MDR-1mRNA and MRP mRNA expression in lung cancer.Adenocarcinoma and squamous cell carcinoma are mainly intrinsic MDR while small cell lung cancer is mainly acquired MDR to chemotherapy.Based on thepositive expression rate of MDR-1 mRNA and MRP mRNA in peripheral blood,we may evaluate the results of chemotherapy in lung cancer patients easily and conveniently.

中图分类号:

R734.2

孔庆暖,傅兴宁,丁晓燕,纪霞,黄维清. 肺癌患者外周血中MDR-1及MRP基因在化疗前后的表达及意义[J]. 实用肿瘤学杂志, 2015, 29(2): 127-132.

KONG Qingnuan,FU Xingning,DING Xiaoyan,JI Xia,HUANG Weiqing. Comparative expression of MDR-1 and MRP in peripheral blood lymphocytes in lung cancer patients with chemotherapy treatment[J]. PRACTICAL ONCOLOGY JOURNAL, 2015, 29(2): 127-132.

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肺癌患者外周血中MDR-1及MRP基因在化疗前后的表达及意义

Comparative expression of MDR-1 and MRP in peripheral blood lymphocytes in lung cancer patients with chemotherapy treatment

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