TRIM5α对肝癌细胞增殖和凋亡的影响

doi:10.11904/j.issn.1002-3070.2016.02.002

实用肿瘤学杂志 ›› 2016, Vol. 30 ›› Issue (2): 103-108.doi: 10.11904/j.issn.1002-3070.2016.02.002

TRIM5α对肝癌细胞增殖和凋亡的影响

余庆, 张文美, 孙迎迎, 龚媛媛, 李东升, 王小莉

湖北医药学院附属太和医院胚胎干细胞研究湖北省重点实验室(十堰 442000)

收稿日期:2016-01-15 出版日期:2016-04-28 发布日期:2016-04-28
通讯作者: 王小莉,E-mail:xiaolitina@126.com
作者简介:余庆,男,(1994-),本科,从事肿瘤基因治疗方面的研究
基金资助:
湖北省自然科学基金(2012FFA037);湖北医药学院自然科学研究启动金(2011QDZR-26)

The effect of TRIM5α on the proliferation and apoptosis of hepatocellular carcinoma cells

YU Qing, ZHANG Wenmei, SUN Yingying, GONG Yuanyuan, LI Dongsheng, WANG Xiaoli

Department of Hubei Key Laboratory of Embryonic Stem Cell Research,Taihe Hospital,Hubei University of Medicine,Shiyan 442000,China

Received:2016-01-15 Online:2016-04-28 Published:2016-04-28

摘要/Abstract

摘要： 目的探讨TRIM5α(Tripartite motif 5 alpha)对人肝癌细胞增殖和凋亡的影响。方法构建TRIM5α质粒,通过酶切、PCR和测序鉴定。将TRIM5α质粒转入人肝癌细胞株(HepG2),通过PCR、Western blot检测TRIM5α的表达。采用RTCA仪器实时检测细胞的增殖情况,采用流式细胞仪检测细胞的凋亡,Western blot检测凋亡相关蛋白的表达。结果本研究成功构建了TRIM5α质粒,RT-PCR和Western blot的结果显示构建的TRIM5α质粒可转入HepG2细胞中并且能够抑制肝癌细胞的增殖促进其凋亡。TRIM5α主要是通过抑制Bcl-2的表达,激活Caspase-3的表达从而引起肝癌细胞凋亡,并且TRIM5α还可抑制肝癌细胞体内成瘤。结论 TRIM5α可抑制人肝癌细胞的增殖促进其凋亡并抑制体内成瘤,为进一步研究其作用机制及其临床应用奠定基础。

关键词: TRIM5α, 肝癌细胞, 增殖, 凋亡

Abstract: Objective To study the effect of Tripartite motif 5 alpha(TRIM5α)on the proliferation and apoptosis of hepatocellular carcinoma cells.Methods The TRIM5α construction was identified by enzyme digestion,PCR and sequencing.The TRIM5α plasmid was transfected into HepG2 cells and identified by RT-PCR and Western blot.The cell proliferation was monitored by RTCA real-time instrument and cell apoptosis was analyzed by flow cytometry.The apoptosis related proteins were detected by Western blot.Results The TRIM5α vector was successfully constructed.The result of RT-PCR and Western blot showed that TRIM5α plasmid could enter HepG2 cells.TRIM5α could inhibit HepG2 cells proliferation also increased their apoptosis through down-regulation of Bcl-2 expression and activation of Caspase-3 expression.TRIM5α also could inhibit the tumor formation of HepG2 cells in vivo.Conclusion TRIM5α inhibited cell proliferation,promoted apoptosis and weakened the tumorigenic ability of HepG2 cells.This will establish the foundation for the mechanism study and the clinic use of TRIM5α for tumor therapy in the future.

Key words: Tripartite motif 5 alpha, Hepatocellular carcinoma cells, Proliferation, Apoptosis

中图分类号:

R73.3

余庆, 张文美, 孙迎迎, 龚媛媛, 李东升, 王小莉. TRIM5α对肝癌细胞增殖和凋亡的影响[J]. 实用肿瘤学杂志, 2016, 30(2): 103-108.

YU Qing, ZHANG Wenmei, SUN Yingying, GONG Yuanyuan, LI Dongsheng, WANG Xiaoli. The effect of TRIM5α on the proliferation and apoptosis of hepatocellular carcinoma cells[J]. PRACTICAL ONCOLOGY JOURNAL, 2016, 30(2): 103-108.

参考文献

1 Tomar D,Singh R.TRIM family proteins:emerging class of RING E3 ligases as regulator of NF-kappaB pathway[J].Biol Cell,2015,107(1):22-40.
2 Versteeg GA,Benke S,Garcia-Sastre A,et al.InTRIMsic immunity:Positive and negative regulation of immune signaling by tripartite motif proteins[J].Cytokine Growth Factor Rev,2014,25(5):563-576.
3 Rajsbaum R,Garcia-Sastre A,Versteeg GA.TRIMmunity:the roles of the TRIM E3-ubiquitin ligase family in innate antiviral immunity[J].J Mol Biol,2014,426(6):1265-1284.
4 Alloush J,Weisleder N.TRIM proteins in therapeutic membrane repair of muscular dystrophy[J].JAMA Neurol,2013,70(7):928-931.
5 Wang J,Zhu J,Dong M,et al.Knockdown of tripartite motif containing 24 by lentivirus suppresses cell growth and induces apoptosis in human colorectal cancer cells[J].Oncol Res,2014,22(1):39-45.
6 Wang Y,He D,Yang L,et al.TRIM26 functions as a novel tumor suppressor of hepatocellular carcinoma and its downregulation contributes to worse prognosis[J].Biochem Biophys Res Commun,2015,463(3):458-465.
7 Shaw RJ.Tumor metabolism:MAGE-a proteins help TRIM turn over AMPK[J].Curr Biol,2015,25(10):418-420.
8 Lukic Z,Campbell EM.The cell biology of TRIM5alpha[J].Curr HIV/AIDS Rep,2012,9(1):73-80.
9 Anderson JS.Using TRIM5alpha as an HIV therapeutic:the alpha gene[J].Expert Opin Biol Ther,2013,13(7):1029-1038.
10 Puvvada MP,Patel SS.Role of trim5alpha in the suppression of cross-species transmission and its defence against human immunodeficiency virus[J].Curr HIV Res,2013,11(8):601-609.
11 Hawthorn L,Stein L,Panzarella J,et al.Characterization of cell-type specific profiles in tissues and isolated cells from squamous cell carcinomas of the lung[J].Lung Cancer,2006,53(2):129-142.
12 Kosaka Y,Inoue H,Ohmachi T,et al.Tripartite motif-containing 29(TRIM29)is a novel marker for lymph node metastasis in gastric cancer[J].Ann Surg Oncol,2007,14(9):2543-2549.
13 Glebov OK,Rodriguez LM,Soballe P,et al.Gene expression patterns distinguish colonoscopically isolated human aberrant crypt foci from normal colonic mucosa[J].Cancer Epidemiol Biomarkers Prev,2006,15(11):2253-2262.
14 Zhang P,Zhang Z,Zhou X,et al.Identification of genes associated with cisplatin resistance in human oral squamous cell carcinoma cell line[J].BMC Cancer,2006,6 224.
15 Yao J,Xu T,Tian T,et al.Tripartite motif 16 suppresses breast cancer stem cell properties through regulation of Gli-1 degradation via the ubiquitin-proteasome pathway[J].Oncol Rep,2016,35(2):1204-1212.
16 Qi J,Ronai ZA.Dysregulation of ubiquitin ligases in cancer[J].Drug Resist Updat,2015,23(3)1-11.
17 Mandell MA,Jain A,Arko-Mensah J,et al.TRIM proteins regulate autophagy and can target autophagic substrates by direct recognition[J].Dev Cell,2014,30(4):394-409.
18 Mandell MA,Kimura T,Jain A,et al.TRIM proteins regulate autophagy:TRIM5 is a selective autophagy receptor mediating HIV-1 restriction[J].Autophagy,2014,10(12):2387-2388.
19 Hsieh MJ,Chen MK,Chen CJ,et al.Glabridin induces apoptosis and autophagy through JNK1/2 pathway in human hepatoma cells[J].Phytomedicine,2016,23(4):359-366.
20 Yu X,Li R,Shi W,et al.Silencing of MicroRNA-21 confers the sensitivity to tamoxifen and fulvestrant by enhancing autophagic cell death through inhibition of the PI3K-AKT-mTOR pathway in breast cancer cells[J].Biomed Pharmacother,2016,77:37-44.

[1]	刘志强,褚艳杰,刘静,王彦博. m白术内酯Ⅰ通过TLR4/MyD88通路调控肺癌A549细胞增殖侵袭的研究[J]. 实用肿瘤学杂志, 2019, 33(3): 228-232.
[2]	刘佳音,燕飞虎,张艳桥. 凋亡基因Caspase3和Caspase7单核苷酸多态性与胃癌遗传易感性研究[J]. 实用肿瘤学杂志, 2019, 33(3): 250-255.
[3]	白晓斌, 霍龙伟, 谢万福, 徐高峰, 王茂德. Chk1在胶质母细胞瘤中的表达及其与肿瘤生物学行为和预后生存的关联性[J]. 实用肿瘤学杂志, 2019, 33(2): 122-127.
[4]	袁桂霞, 李岩, 冯婉琪, 夏小娟, 李旭. 抑癌基因DKK2抑制儿童肾母细胞瘤细胞增殖的机制研究[J]. 实用肿瘤学杂志, 2019, 33(1): 14-20.
[5]	卞秀森, 李光, 关欣宇, 张伊, 狄畅, 马璨. UHRF1通过调控细胞自噬抑制肺腺癌细胞增殖的分子机制研究[J]. 实用肿瘤学杂志, 2018, 32(6): 498-502.
[6]	宋玲, 付琼. 紫云英苷通过抑制HIF-1α诱导的糖酵解途径发挥抗卵巢肿细胞作用的研究[J]. 实用肿瘤学杂志, 2018, 32(6): 503-509.
[7]	刘娟综述, 张澍田审校. 细胞周期蛋白依赖性激酶抑制因子3在肿瘤中的研究进展[J]. 实用肿瘤学杂志, 2018, 32(6): 575-578.
[8]	李万祯, 殷俊, 李萍, 林晓辉, 张国前, 张书旭. iR-17在非小细胞肺癌放射抗拒细胞中的表达及作用研究[J]. 实用肿瘤学杂志, 2018, 32(5): 391-397.
[9]	陈鳞, 王恩湘, 刘群友, 钟鹰, 周国良, 赵喜雁. CDX2基因通过NF-κB信号通路抑制结肠癌细胞增殖[J]. 实用肿瘤学杂志, 2018, 32(5): 398-403.
[10]	陈兆红, 何迎盈, 张友才, 鲁丁瑜. 过表达SOX7对胆囊癌GBC-SD细胞增殖生物学特性的影响及其机制研究[J]. 实用肿瘤学杂志, 2018, 32(4): 293-297.
[11]	李文辉, 吕博文, 钱钧, 苏丽菊, 杨桐树, 钱景荣, 王杰. MicroRNA-150对上皮性卵巢癌细胞增殖、凋亡和侵袭转移能力的影响[J]. 实用肿瘤学杂志, 2018, 32(3): 208-213.
[12]	范宁宁, 耿敬姝. 长链非编码RNA MEG3在人胶质瘤细胞中的表达及作用研究[J]. 实用肿瘤学杂志, 2018, 32(3): 219-223.
[13]	段艳军, 徐卫, 陈文杰, 王凤娟. Wnt通路抑制剂ETC-159处理对口腔鳞癌细胞增殖迁移的影响[J]. 实用肿瘤学杂志, 2018, 32(2): 103-106.
[14]	王笑笑, 华腾, 汪宏波. 雌激素受体相关受体γ在肿瘤中的研究进展[J]. 实用肿瘤学杂志, 2018, 32(1): 87-91.
[15]	孟永生. 番茄红素对人骨肉瘤细胞生长的影响及机制研究[J]. 实用肿瘤学杂志, 2017, 31(6): 506-511.

TRIM5α对肝癌细胞增殖和凋亡的影响

The effect of TRIM5α on the proliferation and apoptosis of hepatocellular carcinoma cells

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价