天然14肽生长抑素联合5-Fu对胃癌移植瘤的抑制作用

doi:10.11904/j.issn.1002-3070.2016.03.004

实用肿瘤学杂志 ›› 2016, Vol. 30 ›› Issue (3): 214-219.doi: 10.11904/j.issn.1002-3070.2016.03.004

天然14肽生长抑素联合5-Fu对胃癌移植瘤的抑制作用

王静¹, 封安强², 张南征²

1.徐州医学院研究生学院(徐州 221002);
2.徐州医学院附属淮海医院消化内科

收稿日期:2016-01-08 出版日期:2016-06-20 发布日期:2016-07-01
通讯作者: 张南征,E-mail:nanzhzhang@sina.com
作者简介:王静,女,(1987-),硕士,住院医师,从事消化系肿瘤的基础与临床工作的研究
基金资助:
军区医学科技创新资助项目(NO.10MA032)

Effect of somatostatin-14 combined with 5-Fu on suppression in human gastric cancer xenografts in nude mice

WANG Jing¹,FENG Anqiang²,ZHANG Nanzheng²

1.Graduate School,Xuzhou Medical College,Xuzhou 221002,China;
2.Department of Gastroenterology,The Affiliated Huaihai Hospital of Xuzhou Medical College

Received:2016-01-08 Online:2016-06-20 Published:2016-07-01

摘要/Abstract

摘要： 目的观察14肽生长抑素(SST-14)及其联合5-Fu对BGC-823、MKN-28人胃癌细胞移植瘤生长抑制及血管内皮生长因子(VEGF)表达的影响。方法构建BGC-823、MKN-28人胃癌细胞移植瘤模型,随机分为对照组、SST-14组、5-Fu组、联合组,腹腔注射药物治疗3周后处死裸鼠获取肿瘤组织。测量瘤体重量,计算抑瘤率;免疫组化及Western blot检测瘤组织VEGF的表达。结果其他三组较对照组均可抑制肿瘤生长,联合组较SST-14、5-Fu单纯用药组抑制作用显著(P＜0.05)。免疫组化及Western blot结果显示,与对照组相比,其他三组瘤组织VEGF表达下降(P＜0.05);其中联合组较SST-14组、5-Fu组显著下降(P＜0.05)。结论 14肽生长抑素联合5-Fu能够有效抑制不同分化程度胃癌细胞移植瘤生长,且优于单纯用药组,两药具有协同抑制作用,可能通过下调VEGF的表达,抑制肿瘤血管生成。

关键词: 14肽生长抑素, 胃癌细胞, 移植瘤, 血管内皮生长因子

Abstract: Objective To observe the effect of Somatostatin-14(SST-14)and SST-14 combined with 5-Fu on the suppressive growth and vessculor endothelial growth factor(VEGF)expression in nude mice subcutaneous xenografts.Methods The animal models of xenotransplanted human gastric cancer were established by implanting BGC-823 and MKN-28 cell lines subcutaneously in nude mice and were randomly divided into 4 groups and treated with normal saline,SST-14,5-Fu and SST-14 combined with 5-Fu for three weeks,respectively.Three weeks after treatment,the animals were killed to take the tumors.The tumor growth inhibition was used to evaluate the suppressive growth.Immunohistochemistry and Western blot were applied to observe the expression of VEGF.Results SST-14 combined with 5-Fu obviously reduced the tumor weight of xenografts(P＜0.05).Immunohistochemical and Western blot indicated that VEGF expression and positive cells in three treatment groups were significantly decreased compared with normal saline group(P＜0.05).Moreover,VEGF expression in combined treatment group was reduced significantly than SST-14,5-Fu groups(P＜0.05).Conclusion Somatostatin-14 combined with 5-Fu significantly suppresses the subcutaneous xenografts growth in nude mice by different human gastric cancer cell lines.The inhibition of angiogenesis by SST-14 together with 5-Fu may through down-regulating the expression of VEGF in subcutaneous xenografts in nude mice.

Key words: Somatostatin-14, Gastric cancer cells, Xenografts, Vascular endothelial growth factor

中图分类号:

R735.2

王静, 封安强, 张南征. 天然14肽生长抑素联合5-Fu对胃癌移植瘤的抑制作用[J]. 实用肿瘤学杂志, 2016, 30(3): 214-219.

WANG Jing,FENG Anqiang,ZHANG Nanzheng. Effect of somatostatin-14 combined with 5-Fu on suppression in human gastric cancer xenografts in nude mice[J]. PRACTICAL ONCOLOGY JOURNAL, 2016, 30(3): 214-219.

参考文献

1 Torre LA,Bray F,Siegel RL,et al.Global cancer statistics,2012[J].CA Cancer J Clin,2015,65(2):87-108.
2 Shen L,Shan YS,Hu HM,et al.Management of gastric cancer in Asia:resource-stratified guidelines[J].Lancet Oncol,2013,14(12):535-547.
3 Patel YC.Somatostatin and its receptor family[J].Front Neuroendocrinol,1999,20(3):157-198.
4 Hofland LJ,Vandamme T,Albertelli M,et al.Hormone and receptor candidates for target and biotherapy of neuroendocrine tumors[J].Front Horm Res,2015,44:216-238.
5 Tejeda M,Gaal D,Hullan L,et al.Evaluation of the antitumor efficacy of the somatostatin structural derivative TT-232 on different tumor models[J].Anticancer Res,2006,26(5a):3477-3483.
6 Xie Y,Chen S,Wang CH,et al.SOM230 combined with celecoxib prolongs the survival in nude mice with HepG-2 xenografts[J].Cancer Biol Ther,2011,12(1):86-92.
7 Theodoropoulou M,Stalla GK.Somatostatin receptors:from signaling to clinical practice[J].Front Neuroendocrinol,2013,34(3):228-252.
8 邵国庆,张南征,江兴松.生长抑素对人胃癌裸鼠种植瘤生长的影响[J].徐州医学院学报,2014,34(6):360-363.
9 邵国庆,张南征.生长抑素对裸鼠人胃癌种植瘤内VEGF和bFGF表达的影响[J].世界华人消化杂志,2014,22(36):5659-5664.
10 Folkman J,Shing Y.Angiogenesis[J].J Biol Chem,1992,267(16):10931-10934.
11 Reinacher-Schick A,Pohl M,Schmiegel W.Drug insight:antiangiogenic therapies for gastrointestinal cancers-focus on monoclonal antibodies[J].Nat Clin Pract Gastroenterol Hepatol,2008,5(5):250-267.
12 Dimova I,Popivanov G,Djonov V.Angiogenesis in cancer-general pathways and their therapeutic implications[J].J buon,2014,19(1):15-21.
13 Zhao DQ,Chen J,Wu YF,et al.Correlation between vascular endothelial growth factor and somatostatin receptor with progression and prognosis in gastric cancer[J].Hepatogastroenterology,2014,61(132):1154-1158.
14 赵滨,杨静,孙勇伟,等.生长抑素对胃癌VEGFs和VEGF受体影响的实验研究[J].实用癌症杂志,2011,26(1):27-30.
15 赵滨,杨静,杨培民,等.生长抑素对胃癌组织中VEGF-C及其受体的影响[J].现代肿瘤医学,2013,21(9):2037-2040.

[1]	代欣综述, 张大昕审校. 非小细胞肺癌抗血管生成治疗研究现状[J]. 实用肿瘤学杂志, 2017, 31(5): 472-476.
[2]	张锐，王智煜，王帅，赵晖. 重组人血管内皮抑素联合NP方案治疗初诊晚期非小细胞肺癌骨转移疗效及对血清VEGF表达的影响[J]. 实用肿瘤学杂志, 2016, 30(6): 527-522.
[3]	陈龙综述, 钱诚审校. 血管内皮生长因子基因多态性与乳腺癌关系的研究进展[J]. 实用肿瘤学杂志, 2016, 30(3): 284-286.
[4]	蒋旸, 耿敬姝. HMGA2在胃癌细胞SGC-7901上皮间质转化中的作用[J]. 实用肿瘤学杂志, 2015, 29(3): 231-234.
[5]	李晓莉,高伊娜,李建慧,王艳颖,马玉彦. TIP30和VEGF-C在小细胞肺癌中的表达及临床意义[J]. 实用肿瘤学杂志, 2015, 29(2): 116-121.
[6]	邱子丹, 李建成. 食管癌靶向治疗研究进展[J]. 实用肿瘤学杂志, 2015, 29(1): 57-60.
[7]	王云萱, 张清媛, 赵红, 解丽, 田文静, 马新星. CXCR7和VEGF在乳腺癌中的表达及其临床意义[J]. 实用肿瘤学杂志, 2013, 27(4): 340-343.
[8]	林梅,夏百荣,高舒. MTDH和VEGF-C在上皮性卵巢癌组织中的表达及其临床病理特征[J]. 实用肿瘤学杂志, 2013, 27(3): 229-233.
[9]	刘婷婷, 韩波. VEGF与非小细胞肺癌放射治疗研究进展[J]. 实用肿瘤学杂志, 2012, 26(6): 564-568.
[10]	张纪妍, 陈杰, 郭兴罡. 四硫化四砷对人卵巢癌裸鼠皮下移植瘤抑瘤作用的研究[J]. 实用肿瘤学杂志, 2012, 26(5): 418-422.
[11]	耿淑美, 赵素彦, 张晓伟, 马旭东, 董静, 贾喜花. 检测腹水中血管内皮生长因子对晚期卵巢癌维持治疗的临床意义[J]. 实用肿瘤学杂志, 2012, 26(5): 443-448.
[12]	朱富娣, 李奇, 张云艳, 尤庆山, 孙静, 陈巍, 吕俊丽. ILK-ASODN对人卵巢上皮癌裸鼠皮下移植瘤形成的抑制作用[J]. 实用肿瘤学杂志, 2012, 26(4): 305-310.
[13]	蒋旸, 佟丹丹, 于晓宇, 冯迪, 谷新悦, 耿敬姝. Snail在TGF-β1诱导胃癌细胞MGC-803上皮间质转化中的作用[J]. 实用肿瘤学杂志, 2012, 26(2): 141-145.
[14]	杨宪广, 孙冀. VEGF研究进展及其在喉癌中的相关研究[J]. 实用肿瘤学杂志, 2011, 25(6): 575-578.
[15]	李陆鹏, 张晶岩, 于友涛, 何东风. TACE对肝细胞癌VEGF、P16表达的影响[J]. 实用肿瘤学杂志, 2011, 25(3): 228-232.

天然14肽生长抑素联合5-Fu对胃癌移植瘤的抑制作用

Effect of somatostatin-14 combined with 5-Fu on suppression in human gastric cancer xenografts in nude mice

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价