芦荟大黄素介导的光动力疗法对人口腔黏膜癌KB细胞杀伤效果的研究

doi:10.11904/j.issn.1002-3070.2016.03.005

实用肿瘤学杂志 ›› 2016, Vol. 30 ›› Issue (3): 220-224.doi: 10.11904/j.issn.1002-3070.2016.03.005

芦荟大黄素介导的光动力疗法对人口腔黏膜癌KB细胞杀伤效果的研究

纪雪钰¹, 臧立新², 王月萍¹, 孟培松¹

1.哈尔滨医科大学附属第四医院口腔科(哈尔滨 150000);
2.哈尔滨工业大学

收稿日期:2016-03-03 出版日期:2016-06-20 发布日期:2016-07-01
通讯作者: 孟培松,E-mail:mengpeisong641205@163.com
作者简介:纪雪钰,女,(1989-),硕士研究生,从事口腔颌面部肿瘤学的研究
基金资助:
哈尔滨市科技局应用技术与开发项目(2014RFXGJ095)

Study of cell killing effect of aloe-emodin mediated photodynamic therapy on KB cells

JI Xueyu¹,ZANG Lixin²,WANG Yueping¹,MENG Peisong¹

1.Department of Stomatology,The Fourth Affiliated Hospital of Harbin Medical University,Harbin 150000,China;
2.Harbin Institute of Technology

Received:2016-03-03 Online:2016-06-20 Published:2016-07-01

摘要/Abstract

摘要： 目的探讨芦荟大黄素(Aloe-emodin,AE)介导的光动力疗法(Photodynamic therapy,PDT)对人口腔黏膜癌KB细胞的杀伤效果。方法通过MTT法检测不同实验条件对KB细胞的杀伤作用。实验分为空白对照组(Control)、单纯药物组(AE)、单纯光照组(Laser)和光动力组(PDT)。分别比较AE组不同浓度(10μM、20μM、30μM和40μM)、Laser组不同光照时间(10s、20s、40s、80s)与Control组对KB细胞的杀伤作用,分析PDT组AE浓度和时间对KB细胞杀伤作用的影响。结果与Control组相比,AE组和Laser组对KB细胞的杀伤作用无统计学意义(P＞0.05)。PDT组对KB细胞的杀伤作用呈浓度和时间依赖性,当AE的浓度为40μM,照射时间为80s时,细胞存活率降低至56.7%。结论 AE介导的PDT对KB细胞有显著的杀伤作用。

关键词: 芦荟大黄素, 光动力疗法, KB细胞, 细胞存活率

Abstract: Objective To investigate the cell killing effect of aloe-emodin(AE)mediated photodynamic therapy(PDT)on human oral mucosa carcinoma KB cells.Methods The cells were randomly divided into four groups:control group;drug alone group;laser alone group and PDT group.The cells were incubated with different AE concentrations(10μM,20μM,30μM and 40μM)for 4h in the dark prior to the treatment,and then treated by PDT with different irradiate times(10s,20s,40s and 80s).The cell killing effect of PDT on KB cells was detected by MTT assay.Results Compared to the control group,neither drug alone group nor laser alone group has significant effect on KB cell(P＞0.05).The cell killing effect of PDT group on KB cells showed dose-dependent and time-dependent inhibition.The cell survival rate decreased in 56.7%,when the AE concentration was 40μM and the laser irradiate time was 80s.Conclusion AE-mediated PDT can be significantly in killing KB cells.

Key words: Aloe-emodin, Photodynamic therapy, KB cell, Cell survival rate

中图分类号:

R739.85

纪雪钰, 臧立新, 王月萍, 孟培松. 芦荟大黄素介导的光动力疗法对人口腔黏膜癌KB细胞杀伤效果的研究[J]. 实用肿瘤学杂志, 2016, 30(3): 220-224.

JI Xueyu,ZANG Lixin,WANG Yueping,MENG Peisong. Study of cell killing effect of aloe-emodin mediated photodynamic therapy on KB cells[J]. PRACTICAL ONCOLOGY JOURNAL, 2016, 30(3): 220-224.

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芦荟大黄素介导的光动力疗法对人口腔黏膜癌KB细胞杀伤效果的研究

Study of cell killing effect of aloe-emodin mediated photodynamic therapy on KB cells

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