结直肠癌肿瘤微环境中调节性T细胞CD39、CD73亚群及二者双阳性亚群的临床意义

doi:10.11904/j.issn.1002-3070.2017.04.008

实用肿瘤学杂志 ›› 2017, Vol. 31 ›› Issue (4): 329-334.doi: 10.11904/j.issn.1002-3070.2017.04.008

结直肠癌肿瘤微环境中调节性T细胞CD39、CD73亚群及二者双阳性亚群的临床意义

王东亮¹,戚宁¹,牟冠男¹,张月桂²

1.哈尔滨医科大学附属肿瘤医院(哈尔滨 150081);
2.哈尔滨医科大学肿瘤研究所

收稿日期:2017-01-09 出版日期:2017-08-20 发布日期:2017-08-23
通讯作者: 王东亮,E-mail:drwangdl@139.com
作者简介:王东亮,男,(1981-),博士,主治医师,从事恶性肿瘤的免疫治疗及综合治疗的研究。
基金资助:
黑龙江省教育厅科学技术研究(面上)项目计划(12541485)

Clinical significance of CD39 CD73 subsets and both of them positive subgroups of regulatory T cells in the microenvironment of colorectal cancer

WANG Dongliang¹,QI Ning¹,MU Guannan²,ZHANG Yuegui²

1.Harbin Medical University Cancer Hospital,Harbin 150081,China;
2.Harbin Medical University Cancer Institute

Received:2017-01-09 Online:2017-08-20 Published:2017-08-23

摘要/Abstract

摘要： 目的观察结直肠癌肿瘤组织微环境中浸润的调节性T细胞的临床意义,并探究其CD39、CD73及CD39,CD73双阳性亚群与肿瘤微环境中其他淋巴细胞及临床病理参数之间的关系。方法收集24例结直肠癌患者根治术后组织标本,剪碎并分离培养组织内肿瘤浸润淋巴细胞(Tumor infiltrating lymphocyte,TIL),以流式细胞术观察调节性T细胞CD39⁺、CD73⁺及CD39⁺CD73⁺亚群与患者临床病理参数之间的关联。结果结直肠癌肿瘤组织微环境中浸润的调节性T细胞CD73⁺亚群,CD39⁺CD73⁺亚群与淋巴结转移及分化程度不良具有相关性,并且其机制与肿瘤相关性炎症密切相关。结论相比Treg中的其他亚群,CD39⁺CD73⁺阳性的结直肠肿瘤浸润Treg具有更为独特的生物学活性,本研究为预测结直肠癌预后提供了新思路与理论依据。

关键词: 结直肠癌, 肿瘤浸润的淋巴细胞, 调节性T细胞, CD39, CD73

Abstract: Objective The objectives of this study were to investigate the clinical significance of CD39,CD73,double positive subgroups for CD39 and CD73,and other lymphocytes with clinicopathological parameters in the microenvironment of colorectal cancer.Methods Tumor infiltrating lymphocyte(TIL)was collected from 24 patients with colorectal cancer after radical resection.The expression of CD39⁺,CD73⁺ or CD39⁺ with CD73⁺ in T cells were measured by flow cytometry.The association between these subgroups and clinicopathologic parameters was analyzed.Results The CD73⁺ and CD39⁺ with CD73⁺ subgroups were associated with lymph node metastasis and poor degree of differentiation,and this mechanism was closely related to tumor-associated inflammation.Conclusion CD39⁺ with CD73⁺ colorectal tumor infiltration Treg has a more unique biological activity than other Treg group.This study provides a new idea and theoretical basis for predicting the prognosis of colorectal cancer.

Key words: Colon cancer, Tumor-infiltrating lymphocyte, Regulatory T lymphocyte , CD39, CD73

中图分类号:

R574.62

王东亮,戚宁,牟冠男,张月桂. 结直肠癌肿瘤微环境中调节性T细胞CD39、CD73亚群及二者双阳性亚群的临床意义[J]. 实用肿瘤学杂志, 2017, 31(4): 329-334.

WANG Dongliang ,QI Ning ,MU Guannan ,ZHANG Yuegui. Clinical significance of CD39 CD73 subsets and both of them positive subgroups of regulatory T cells in the microenvironment of colorectal cancer[J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(4): 329-334.

参考文献

1 Siegel RL,Miller KD,Fedewa SA,et al.Colorectal cancer statistics,2017[J].CA Cancer J Clin,2017,67(3):177-193.
2 Chen W,Zheng R,Baade PD,et al.Cancer statistics in China,2015[J].CA Cancer J Clin,2016,66(2):115-132.
3 Donnem T,Kilvaer TK,Andersen S,et al.Strategies for clinical implementation of TNM-Immunoscore in resected nonsmall-cell lung cancer[J].Ann Oncol,2016,27(2):225-232.
4 Takeuchi Y,Nishikawa H.Roles of regulatory T cells in cancer immunity[J].Int Immunol,2016,28(8):401-409.
5 Ibrahim EM,Al-Foheidi ME,Al-Mansour MM,The prognostic value of tumor-infiltrating lymphocytes in triple-negative breast cancer:a meta-analysis[J].Breast Cancer Res Treat,2014,148(3):467-476.
6 Singh M,Loftus T,Webb E,et al.Minireview:Regulatory T cells and ovarian cancer[J].Immunol Invest,2016,45(8):712-720.
7 Zhuo C,Xu Y,Ying M,et al.FOXP3⁺ Tregs:heterogeneous phenotypes and conflicting impacts on survival outcomes in patients with colorectal cancer[J].Immunol Res,2015,61(3):338-347.
8 Shang B,Liu Y,Jiang SJ,et al.Prognostic value of tumor-infiltrating FoxP3+ regulatory T cells in cancers:a systematic review and meta-analysis[J].Sci Rep,2015,14(5):15179.
9 Renner K,Singer K,Koehl GE,et al.Metabolic hallmarks of tumor and immune cells in the tumor microenvironment[J].Front Immunol,2017,8(8):248.
10 Solinas C,Pusole G,Demurtas L,et al.Tumor infiltrating lymphocytes in gastrointestinal tumors:Controversies and future clinical implications[J].Crit Rev Oncol Hematol,2017,110:106-116.
11 Allard B,Longhi MS,Robson SC,et al.The ectonucleotidases CD39 and CD73:Novel checkpoint inhibitor targets[J].Immunol Rev,2017,276(1):121-144.
12 de Oliveira Bravo M,Carvalho JL,Saldanha-Araujo F.Adenosine production:a common path for mesenchymal stem-cell and regulatory T-cell-mediated immunosuppression[J].Purinergic Signal,2016,12(4):595-609.
13 Wang DL,Liu YY,Gu YL,et al.Increased number of forkhead box P3+ tumor-infiltrating lymphocytes correlates with high preoperative albumin level and better survival in patients with stage Ⅱ or Ⅲ colorectal cancer[J].Tumour Biol,2015,36(7):5407-5414.

[1]	方琳, 刘超, 张纯慧, 张艳桥. 转移性结直肠癌维持治疗的研究现状与治疗策略[J]. 实用肿瘤学杂志, 2019, 33(2): 173-178.
[2]	佟广海, 田洋, 吴华星. 脂质组学在结直肠癌研究中的应用[J]. 实用肿瘤学杂志, 2019, 33(2): 179-192.
[3]	刘恒辰, 张新宇. Toll样受体信号通路作为结直肠癌潜在治疗靶点的研究进展[J]. 实用肿瘤学杂志, 2019, 33(2): 188-192.
[4]	刘宏涛, 张彤彤综述, 刘雁军审校. 肥胖与结直肠癌发病机制的研究进展[J]. 实用肿瘤学杂志, 2018, 32(5): 464-467.
[5]	王志, 刘忠卫, 曾雪芸, 隋明洁, 廖静怡, 李月欢, 黄卫东, 刘国祥. 基于EQ-5D-5L的结直肠癌癌前病变患者健康相关生命质量研究[J]. 实用肿瘤学杂志, 2018, 32(4): 320-326.
[6]	崔雪阳, 邢承忠. 液体活检在结直肠癌领域的研究及应用现状[J]. 实用肿瘤学杂志, 2018, 32(4): 367-370.
[7]	李文琪, 耿敬姝. RAS/RAF突变与MSI状态在结直肠癌治疗中的研究进展[J]. 实用肿瘤学杂志, 2018, 32(4): 371-376.
[8]	张姗姗, 吴华星审校. 代谢组学在结直肠癌诊断中的应用[J]. 实用肿瘤学杂志, 2017, 31(6): 573-576.
[9]	孙丽娜, 赵晓迪综述, 卢瑗瑗, 王新审校. LncRNA与胃肠道肿瘤化疗耐药的研究进展[J]. 实用肿瘤学杂志, 2017, 31(5): 448-453.
[10]	郝小军，王传卓，赵相轩，辛鹤，刘兆玉. miR-34a对结肠癌细胞SW480增殖、迁移和侵袭能力的影响及机制探讨[J]. 实用肿瘤学杂志, 2017, 31(3): 193-198.
[11]	张明, 朱信强, 丁闯, 孔令永, 陈焰. 结直肠癌患者术后预后营养指数在预后评估中的应用[J]. 实用肿瘤学杂志, 2017, 31(2): 131-136.
[12]	苗大壮, 佟金学, 贾云鹤. 加速康复外科理念在腹腔镜辅助结直肠癌手术围手术期中的应用价值[J]. 实用肿瘤学杂志, 2017, 31(2): 137-141.
[13]	刘珈杞，刘耀华，陈鑫，综述，赵世光，审校. CD4⁺CD25⁺Foxp3⁺Treg细胞调控肿瘤免疫抑制微环境的研究进展[J]. 实用肿瘤学杂志, 2016, 30(6): 555-559.
[14]	崔君鹏, 刘宝林. VDR基因FokI位点多态性与结直肠癌易感性之间关系的Meta分析[J]. 实用肿瘤学杂志, 2016, 30(5): 400-408.
[15]	杨晓宏, 靳英综述, 史增祥审校. 代谢综合征与结直肠癌的相关性研究[J]. 实用肿瘤学杂志, 2016, 30(2): 146-149.

结直肠癌肿瘤微环境中调节性T细胞CD39、CD73亚群及二者双阳性亚群的临床意义

Clinical significance of CD39 CD73 subsets and both of them positive subgroups of regulatory T cells in the microenvironment of colorectal cancer

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价