原苏木素A对胃癌SGC-7901细胞系放疗增敏作用的研究

doi:10.11904/j.issn.1002-3070.2017.06.004

实用肿瘤学杂志 ›› 2017, Vol. 31 ›› Issue (6): 500-505.doi: 10.11904/j.issn.1002-3070.2017.06.004

原苏木素A对胃癌SGC-7901细胞系放疗增敏作用的研究

刘国慧, 谷安鑫, 殷洪涛, 曹阳, 贺云龙, 王春波, 鄂明艳

哈尔滨医科大学附属肿瘤医院胸部放疗二病区(哈尔滨 150081)

收稿日期:2017-05-21 出版日期:2017-12-28 发布日期:2018-01-02
通讯作者: 鄂明艳,E-mail:emingyansci@126.com
作者简介:刘国慧,女,(1989),硕士,住院医师,从事胸腹部肿瘤放射治疗的研究。

Protosappanin A increases the sensitivity of gastric cancer SGC-7901 cells to radiotherapy

LIU Guohui, GU Anxin, YIN Hongtao, CAO Yang, HE Yunlong, WANG Chunbo, E Mingyan

Department of Radiation Oncology,Harbin Medical University Cancer Hospital,Harbin 150081,China

Received:2017-05-21 Online:2017-12-28 Published:2018-01-02

摘要/Abstract

摘要： 目的本研究将原苏木素A、苏木醇提物、顺铂分别与放疗联合作用于胃癌细胞SGC-7901细胞系,研究原苏木素A是否增加胃癌SGC-7901细胞系放射敏感性,为临床用药开创更新的领域。方法体外培养人胃癌细胞SGC-7901细胞系,以苏木醇提物、顺铂为对照,MTT法检测原苏木素A对细胞增殖作用的影响,以及其与作用浓度和作用时间的关系;采用克隆形成实验,拟合细胞存活曲线,以单纯放疗组为对比,计算放射增比(SER),分析原苏木素A的增敏效果。结果原苏木素A对人胃癌细胞SGC-7901有生长抑制作用,但抑制作用相对较弱,其细胞毒性有明显的时间和浓度依赖性。不同作用时间及作用浓度下的细胞形态发生明显的改变。克隆形成实验显示原苏木素A显著增加了SGC-7901细胞的放射敏感性,与单纯放射组对比,差异有统计学意义。结论原苏木素A对胃癌SGC-7901细胞的抑制有浓度和时间的依赖性,联合放射治疗能够提高放射增敏作用,两者可能具有协同抗肿瘤作用。

关键词: 原苏木素A, 苏木醇提物, 胃癌, 放射治疗, 放射敏感性

Abstract: Objective In this study,Protosappanin A,Caesalpinia Sappan L extract and Cisplatin were combined with radiotherapy in gastric cancer cell SGC-7901 to investigate whether the Protosappanin A could increase radiosensitivity(SER)in gastric cancer SGC-7901 cells.This will be medication to create new areas of innovation in the future.Methods The cell proliferation of SGC-7901 cells was detected by MTT assay.The relationship between the effect of the Protosappanin A on cell proliferation and the time of action was determined.Caesalpinia Sappan L extract and Cisplatin were as controls.The fitted cell survival curve and clonal formation assays were used to determine the SER to analyze the sensitizative effect of Protosappanin A.Results Protosappanin A could inhibit the growth of SGC-7901 cells,and its inhibitory effect is relatively weak.Its cytotoxicity has a time-and concentration-dependent manner.Cellular morphological changes were observed accompanying with increased concentrations and time treatments of Protosappanin A.Clonal formation experiment showed that the Protosappanin A significantly increased the radiosensitivity of SGC-7901 cells when compared to the radioactive group.They showed a statistically difference.Conclusion The inhibitory effect of the Protosappanin A on SGC-7901 cells in a concentration and time-dependent manner.Protosappanin A combined radiotherapy can improve the radiosensitization of cells,both of which may have synergistic anti-tumor effect.

Key words: Protosappanin A, Caesalpinia Sappan L extract, Gastric cancer, Radiotherapy, Radiosensitivity

中图分类号:

刘国慧, 谷安鑫, 殷洪涛, 曹阳, 贺云龙, 王春波, 鄂明艳. 原苏木素A对胃癌SGC-7901细胞系放疗增敏作用的研究[J]. 实用肿瘤学杂志, 2017, 31(6): 500-505.

LIU Guohui, GU Anxin, YIN Hongtao, CAO Yang, HE Yunlong, WANG Chunbo, E Mingyan. Protosappanin A increases the sensitivity of gastric cancer SGC-7901 cells to radiotherapy[J]. PRACTICAL ONCOLOGY JOURNAL, 2017, 31(6): 500-505.

参考文献

1 Chen W,Zheng R,Baade PD,et al.Cancer statistics in China,2015[J].CA Cancer J Clin,2016,66(2):115-132.
2 季加孚.我国胃癌防治研究三十年回顾[J].中国肿瘤临床,2013,40(22):1345-1351.
3 徐建国,郭素堂,乔丽娟,等.苏木提取液抑制肿瘤作用的研究[J].肿瘤研究与临床,2006,11(3):726-728.
4 张静文.苏木药理作用研究进展[J].现代医药卫生,2008,16:234-237.
5 欧阳波.苏木化学成分研究[D].上海:中国科学院上海药物研究所,2002:17-21.
6 Zuo GY,Han ZQ,Han J,et al.Antimicrobial activity and synergy of antibiotics with two biphenyl compounds,protosappanins A and B from Suppuu Liguum against methicillin-resistant Staphylococcus aureus strains[J].J Pharm Pharmacol,2015,67(10):1439-1447.
7 Parkin DM,Bray F,Feraly J,et al.Global cancer statistics,2002[J].CA Cancer J Clin,2005,55(2):74-108.
8 舒诗会,张莉,杜冠华,等.苏木的化学成分研究[J].天然产物研究与开发,2007,19(1):63-66.
9 Kim EC,Hwang YS,Lee HJ,et al.Caesalpinia sappan induces cell death by increaming the expression of P53 and P21WAF1/CIPI in head and neck cancer cells[J].Am J Chin Med,2005,33(3):405-414.
10 Jemal A,Siegel R,Xu J,et al.Cancer statistics,2010[J].CA Cancer J Clin,2010,60(5):277-300.
11 Tian T,Zhang PT,Yu MW,et al.Effect of hematoxylin in C57BL/6 mice with Lewis lung cancer growth and metastasis of different times[J].Chin J Integr Med,2010,30(7):733-737.
12 Qiao LJ,Xu JG,Guo WJ,et al.Experimental study of the active ingredient of anti-cancer hematoxylin transplanted hepatoma H22[J].Hainan Medicine,2001,12(7):51-52.
13 Tian T,Zhang PT.Experimental study of bone marrow hematoxylin CK18 CK19 expression on C57/BL6 tumor-bearing mice[J].Beijing Chinese Medicine,2010,29(3):222-224.
14 Lange H.An overview of cancer multidurg resistance:a still unsolved problem[J].Cell Mol Life Sci,2008,65(20):3145-3167.
15 Gillet JP,Efferth T,Reamacle J,et al.Chemotherapy-induced resistance by ATP-binding cassette transporter genes[J].Biochim Biophys Acta,2007,1775(2):237-262.
16 Marchi N,Gonzalez-Martinez J,Njuyen MT,et al.Transporters in drug-refractory epilepsy;clinical significance[J].Clin Pharmacol Ther,2010,87(1):13-15.
17 Tao LY,Li JY.Research ABCB1-mediated multidrug resistance activity inhibits oxidation hematoxylin[J].Liaoning Medical Journal,2011,32(3):217-220.

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原苏木素A对胃癌SGC-7901细胞系放疗增敏作用的研究

Protosappanin A increases the sensitivity of gastric cancer SGC-7901 cells to radiotherapy

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