肺癌完全性切除术对患者外周血中Th17/Treg细胞平衡的影响

doi:10.11904/j.issn.1002-3070.2019.02.009

实用肿瘤学杂志 ›› 2019, Vol. 33 ›› Issue (2): 142-148.doi: 10.11904/j.issn.1002-3070.2019.02.009

肺癌完全性切除术对患者外周血中Th17/Treg细胞平衡的影响

赵苏¹, 郑晓瑜², 朱开彬¹, 卜建龙¹, 徐世东¹

1.哈尔滨医科大学附属肿瘤医院胸外科(哈尔滨 150081);
2.哈尔滨医科大学附属肿瘤医院麻醉科

收稿日期:2018-12-13 修回日期:2019-01-14 出版日期:2019-04-20 发布日期:2019-04-25
通讯作者: 徐世东,E-mail:xushidonghyd@163.com
作者简介:赵苏,男,(1990-),硕士,住院医师,从事肺癌临床的研究

Effect of complete lung cancer resection on the balance of Th17/Treg in the peripheral blood of patients

ZHAO Su¹, ZHENG Xiaoyu², ZHU Kaibin¹, BU Jianlong¹, XU Shidong¹

1.Department of Thoracic Surgery, Harbin Medical University Cancer Hospital, Harbin 150081, China;
2.Department of Anesthesiology, Harbin Medical University Cancer Hospital

Received:2018-12-13 Revised:2019-01-14 Online:2019-04-20 Published:2019-04-25

摘要/Abstract

摘要： 目的探讨肺癌完全性切除术对肺癌患者外周血中Th17/Treg细胞平衡的影响。方法流式细胞术检测21例肺癌患者术前、术后和21名健康对照者外周血中Th17、Treg细胞的百分比,RT-PCR法检测PBMC中转录因子孤独核受体(Retinoic acid-related orphan receptor γt,RORγt)、叉头翼状旋转转录因子(Fork-head/winged helix transcription,Foxp3)的表达。ELISA法检测血浆中IL-17和TGF-β1的水平。结果手术后患者相比术前外周血中Th17细胞百分比减少,而Treg细胞百分比增加(P＜0.01),手术后患者相比术前CD4+T细胞中RORγt表达量降低,而Foxp3表达量升高(P＜0.01),手术后患者相比于术前血浆中IL-17的表达降低(P＜0.01),而TGF-β1的表达增加(P＜0.01)。此外,术前的肺癌患者和健康对照者相比,外周血中Th17和Treg细胞百分比、RORγt和Foxp3表达量以及IL-17和TGF-β表达水平均增加。结论肺癌完全性切除术后肺癌患者外周血中存在Th17/Treg失衡。

关键词: 肺癌完全性切除术, 免疫抑制, Th17, Treg, RORγt, Foxp3

Abstract: Objective The aim of this study was to investigate the effect of complete lung cancer resection on the balance of Th17/Treg cells in the peripheral blood of lung cancer patients. Methods Flow cytometry was used to detect the percentage of Th17 and Treg cells in the peripheral blood of 21 patients with lung cancer before and after operation and 21 healthy controls.RT-PCR was used to detect the expression of fork-head/winged helix transcription factor(Foxp3)and retinoic acid-related orphan receptor γt(RORγt)in PBMC.The plasma levels of interleukin(IL)-17 and transforming growth factor(TGF)-β1 were detected by enzyme-linked immunosorbent assay(ELISA). Results After surgery,the percentage of Th17 cells in the peripheral blood decreased and the percentage of Treg cells increased in patients when compared to the pre-operation(P＜0.01).After surgery,the expression of RORγt was decreased and the expression of Foxp3 was increased in the CD4⁺T cells of patients in comparison with the pre-operation(P＜0.01).After surgery,the expression of IL-17 was decreased and the expression of TGF-β1 was increased in the plasma of patients in comparison with the pre-operation(P＜0.01).In addition,the percentage of Th17 and Treg cells,the expression of RORγt and Foxp3,and the expression of IL-17 and TGF-β in the peripheral blood were increased in preoperative lung cancer patients when compared to healthy controls. Conclusion There is a Th17/Treg imbalance in the peripheral blood of lung cancer patients after complete resection of lung cancer.

Key words: Lung cancer resection, Immunosuppression, Th17, Treg, RORγt, Foxp3

中图分类号:

R734.2

赵苏, 郑晓瑜, 朱开彬, 卜建龙, 徐世东. 肺癌完全性切除术对患者外周血中Th17/Treg细胞平衡的影响[J]. 实用肿瘤学杂志, 2019, 33(2): 142-148.

ZHAO Su, ZHENG Xiaoyu, ZHU Kaibin, BU Jianlong, XU Shidong. Effect of complete lung cancer resection on the balance of Th17/Treg in the peripheral blood of patients[J]. PRACTICAL ONCOLOGY JOURNAL, 2019, 33(2): 142-148.

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肺癌完全性切除术对患者外周血中Th17/Treg细胞平衡的影响

Effect of complete lung cancer resection on the balance of Th17/Treg in the peripheral blood of patients

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