激发性CD28单抗对肿瘤患者外周血FOXP3+Treg细胞的下调作用及意义

doi:10.3969/j.issn.1002-3070.2009.02.004

实用肿瘤学杂志 ›› 2009, Vol. 23 ›› Issue (2): 113-117.doi: 10.3969/j.issn.1002-3070.2009.02.004

激发性CD28单抗对肿瘤患者外周血FOXP3⁺Treg细胞的下调作用及意义

杜阳阳,沈丽琴,王艳茹,刘玉华,孙杰,庄志祥,邱玉华

苏州大学附属第二医院肿瘤科(苏州 215004)

收稿日期:2009-02-19 出版日期:2009-04-20 发布日期:2012-02-21
作者简介:杜阳阳,女,(1984-),硕士研究生,从事肿瘤的生物治疗

The role and significance of CD28 monoclonal antibody stimulating peripheral blood of patients with tumors of FOXP3⁺Treg cells

DU Yangyang ,Shen Liqin,WANG Yanru,LIU Yuhua,SUN Jie,ZHUANG Zhixiang,QIU Yuhua

Suzhou University Medical Biotechnology Research Institute,Suzhou 215007

Received:2009-02-19 Online:2009-04-20 Published:2012-02-21

摘要/Abstract

摘要： 目的探究肿瘤患者外周血单个核细胞(PBMC)中FOXP3⁺Treg经CD28单抗激发后的改变及意义。方法采用腹水诱生法及免疫亲和层析法进行单抗的制备和纯化,Ficoll密度梯度离心法获取外周血PBMC,经激发性CD28单抗10μg/mL共培养后经免疫荧光及流式细胞术分析其中FOXP3⁺Treg的表达以及FOXP3⁺Fas⁺Treg和FOXP3⁺FasL⁺Treg的表达。结果免疫荧光和流式细胞术显示肿瘤患者外周血中FOXP3⁺Treg表达比例为(6.208±2.754)%,而对照组为(2.653±0.638)%。FOXP3⁺FasL⁺Treg细胞经CD28单抗活化后FasL表达上调为(7.252±2.23)%,而健康对照组为(7.96±2.938)%,FOXP3⁺Fas⁺Treg活化前后的比例分别为(1.467±0.590)%和(3.692±1.822)%。结论肿瘤患者外周血中PBMC经CD28单抗激发后FOXP3⁺Treg的比例下调(P＜0.01),提示该抗体能够使肿瘤患者的免疫功能得到一定程度的恢复与提高。

Abstract: Objective To explore the change and significance of FOXP3⁺Treg within peripheral blood mononudear cells(PBMC),which stimulated by CD28 monoclonal antibody in tumor patients.Methods Ascites induced immune and pro-law chromatography for purification and preparation of the monoclonal antibody,Ficoll density gradient centrifugation to obtain peripheral blood PBMC,and/or stimulated by CD28 monoclonal antibody 10μg/mL after co-culture by immunofluorescence and flow cytometry analysis of FOXP3⁺ Treg,as well as the expression of FOXP3⁺Fas⁺Treg and FOXP3⁺ FasL⁺Treg.Results Immunofluorescence and flow cytometry showed that the peripheral blood of tumor patients with FOXP3⁺Treg expression ratio(6.208±2.754)%,while the control group(2.653±0.638)%.FOXP3+FasL+Treg cells activated by CD28 monoclonal antibody for the FasL expression(7.252± 2.23)%,and healthy control group(7.96± 2.938)% Before and after activation the ratio of FOXP3⁺Fas⁺Treg(1.467± 0.590)%,(3.692± 1.822)%.Conclusion The ratio of FOXP3⁺Treg within PBMC in tumor patients decreased(P＜0.01)after stimulating by CD28 monoclonal antibody which suggested the patients immune function recover and improve.

中图分类号:

R392.1

杜阳阳,沈丽琴,王艳茹,刘玉华,孙杰,庄志祥,邱玉华. 激发性CD28单抗对肿瘤患者外周血FOXP3⁺Treg细胞的下调作用及意义[J]. 实用肿瘤学杂志, 2009, 23(2): 113-117.

DU Yangyang ,Shen Liqin,WANG Yanru,LIU Yuhua,SUN Jie,ZHUANG Zhixiang,QIU Yuhua. The role and significance of CD28 monoclonal antibody stimulating peripheral blood of patients with tumors of FOXP3⁺Treg cells[J]. PRACTICAL ONCOLOGY JOURNAL, 2009, 23(2): 113-117.

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激发性CD28单抗对肿瘤患者外周血FOXP3⁺Treg细胞的下调作用及意义

The role and significance of CD28 monoclonal antibody stimulating peripheral blood of patients with tumors of FOXP3⁺Treg cells

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激发性CD28单抗对肿瘤患者外周血FOXP3+Treg细胞的下调作用及意义

The role and significance of CD28 monoclonal antibody stimulating peripheral blood of patients with tumors of FOXP3+Treg cells

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激发性CD28单抗对肿瘤患者外周血FOXP3⁺Treg细胞的下调作用及意义

The role and significance of CD28 monoclonal antibody stimulating peripheral blood of patients with tumors of FOXP3⁺Treg cells