ILF2及NF90在口腔鳞状细胞癌中的表达及临床意义

doi:10.11904/j.issn.1002-3070.2022.02.007

实用肿瘤学杂志 ›› 2022, Vol. 36 ›› Issue (2): 137-143.doi: 10.11904/j.issn.1002-3070.2022.02.007

ILF2及NF90在口腔鳞状细胞癌中的表达及临床意义

王雪¹, 高雨蔚¹, 宋红权¹, 张兴伟¹, 杨帆², 焦晓辉¹

1.哈尔滨医科大学附属第一医院口腔颌面外科(哈尔滨 150001);
2.黑龙江中医药大学附属第一医院口腔科

收稿日期:2021-11-08 修回日期:2022-02-22 出版日期:2022-04-28 发布日期:2022-04-28
通讯作者: 焦晓辉,E-mail:jiaoxiaohuidoctor@163.com
作者简介:王雪,女,(1996-),硕士,住院医师,从事口腔鳞癌发生机制与诊断方面的研究。
基金资助:
科技部十三五国家重点研发计划项目(编号:2016YFC1000504)

Expression and clinical significance of ILF2 and NF90 in oral squamous cell carcinoma

WANG Xue¹, GAO Yuwei¹, SONG Hongquan¹, ZHANG Xingwei¹, YANG Fan², JIAO Xiaohui¹

1. Department of Oral and Maxillofacial Surgery,The First Affiliated Hospital of Harbin Medical University,Harbin 150001,China;
2. Department of Stomatology,The First Affiliated Hospital of Heilongjiang University of Traditional Chinese Medicine

Received:2021-11-08 Revised:2022-02-22 Online:2022-04-28 Published:2022-04-28

摘要/Abstract

摘要： 目的探讨白细胞介素增强结合因子2(Interleukin enhancer-binding factor 2,ILF2)及核因子90(Nuclear factor 90,NF90)在口腔鳞状细胞癌(Oral squamous cell carcinoma,OSCC)中的表达及临床意义。方法使用免疫组织化学(Immunohistochemistry,IHC)及实时荧光定量检测(Quantitative real-time PCR,qRT-PCR)方法检测ILF2及NF90在OSCC和正常口腔黏膜(Normal oral mocusa,NOM)组织中的表达水平,并进一步分析二者表达情况与OSCC患者临床病理特征以及预后之间的关系;使用Oncomine及GEPIA数据库分析二者在不同组织中的表达情况。结果与NOM组织对比,ILF2及NF90在OSCC组织中高表达(P＜0.05),二者在OSCC组织中的表达具有相关性(φ=0.540,P＜0.001);Oncomine数据库结果显示ILF2及NF90同样在OSCC组织中高表达(P＜0.05);GEPIA数据库结果显示ILF2及NF90在OSCC中的表达呈正相关(r=0.600,P＜0.001)。ILF2及NF90的表达情况与OSCC的肿瘤分化程度、TNM分期及淋巴结是否转移相关(P＜0.05);生存分析显示ILF2和NF90高表达的患者总生存期较短,且ILF2及NF90因子的高表达和肿瘤分化程度是影响患者总生存期的独立危险因素(P＜0.05)。结论 ILF2和NF90在OSCC中高表达,且两者具有相关性,对OSCC患者的预后有不良影响,提示二者可以作为OSCC发生发展新的诊断标志物及预后评估指标。

关键词: 口腔鳞状细胞癌, 白细胞素增强结合因子2, 核因子90

Abstract: Objective The aim of this study was to investigate the expression of interleukin enhancer binding factor 2(ILF2)and nuclear factor 90(NF90)in oral squamous cell carcinoma(OSCC)and their clinical significance.Methods Immunohistochemistry(IHC)and quantitative real-time PCR(qRT-PCR)were used to detect the expression of ILF2 and NF90 in OSCC and normal oral mucosa(NOM)tissues.The relationship between their expression and clinicopathological features and prognosis of OSCC patients was further analyzed.Oncomine and GEPIA databases were used to analyze their expression in different tissues.Results Compared with NOM tissues,ILF2 and NF90 were highly expressed in OSCC tissues(P＜0.05);their expression was positively correlated in OSCC tissues(φ=0.540,P＜0.001);the results of Oncomine database showed that ILF2 and NF90 were also highly expressed in OSCC tissues(P＜0.05);The results of GEPIA database showed that the expression of ILF2 and NF90 in OSCC was positively correlated(r=0.600,P＜0.001).The expression of ILF2 and NF90 was correlated with the degree of tumor differentiation,TNM stage and lymph node metastasis of OSCC(P＜0.05).Prognostic survival analysis showed that patients with high expression of ILF2 and NF90 had a short overall survival,and the high expression of ILF2 and NF90 and the degree of tumor differentiation were independent risk factors affecting the overall survival of patients.Conclusion ILF2 and NF90 are highly expressed and correlated in OSCC,and they are correlated,and have adverse effects on the prognosis of OSCC patients,suggesting that they can be used as new diagnostic markers and prognostic indicators for the occurrence and development of OSCC

Key words: Oral squamous cell carcinoma, Leukocytokinin enhancing binding factor 2, Nuclear factor 90

中图分类号:

R782

王雪, 高雨蔚, 宋红权, 张兴伟, 杨帆, 焦晓辉. ILF2及NF90在口腔鳞状细胞癌中的表达及临床意义[J]. 实用肿瘤学杂志, 2022, 36(2): 137-143.

WANG Xue, GAO Yuwei, SONG Hongquan, ZHANG Xingwei, YANG Fan, JIAO Xiaohui. Expression and clinical significance of ILF2 and NF90 in oral squamous cell carcinoma[J]. Journal of Practical Oncology, 2022, 36(2): 137-143.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://journal09.magtechjournal.com/Jwk3_syzlxzz/CN/10.11904/j.issn.1002-3070.2022.02.007

http://journal09.magtechjournal.com/Jwk3_syzlxzz/CN/Y2022/V36/I2/137

参考文献

1 Li YY,Wang YT,Li J,et al.Tacrolimus inhibits oral carcinogenesis through cell cycle control[J].Biomed Pharmacother,2021,139:111545.
2 Sung H,Ferlay J,Siegel RL,et al.Global cancer statistics 2020:GLOBOCAN estimates of incidence and mortality worldwide for 36 Cancers in 185 countries[J].CA Cancer J Clin,2021,71(3):209-249.
3 王哲,祝率,刘欢,等.SRPX2和Rab31在口腔鳞癌组织中的表达、相关性及临床意义[J].实用肿瘤学杂志,2021,35(5):414-419.
4 Marcoulatos P,Koussidis G,Mamuris Z,et al.Mapping interleukin enhancer binding factor 2 gene(ILF2)to human chromosome 1(1q11-qter and 1p11-p12)by polymerase chain reaction amplification of human-rodent somatic cell hybrid DNA templates[J].Interferon Cytokine Res,1996,16(12):1035-1038.
5 Marcoulatos P,Avgerinos E,Tsantzalos DV,et al.Mapping interleukin enhancer binding factor 3 gene(ILF3)to human chromosome 19(19q11-qter and 19p11-p13.1)by polymerase chain reaction amplification of human-rodent somatic cell hybrid DNA templates[J].Interferon Cytokine Res,1998,18(5):351-355.
6 Masuda K,Kuwano Y,Nishida K,et al.NF90 in posttranscriptional gene regulation and microRNA biogenesis[J].Int J Mol Sci,2013,14(8):17111-17121.
7 张超杰,闫红娟,赵举红,等.口腔鳞癌中FOXP3的表达及临床意义[J].实用医学杂志,2021,37(7):939-943.
8 王树斌,秦帅华,易雅群,等.216例口腔鳞癌患者术后心理困扰影响因素及放疗前、后生存质量评价[J].上海口腔医学,2021,30(1):71-76.
9 Ni TM,Guo X,Xue Q,et al.Upregulated expression of ILF2 in non-small cell lung cancer is associated with tumor cell proliferation and poor prognosis[J].J Mol Histol,2015,46:4-5.
10 姚文健.NF45在食管鳞状细胞癌中的生物学作用及机制研究[D].湖北:华中科技大学,2019.
11 Jin Z,Xu L,Zhang L,et al.Interleukin enhancer binding factor 2 is a prognostic biomarker for breast cancer that also predicts neoadjuvant chemotherapy responses.[J].Am J Transl Res,2018,10(6):1677-1689.
12 Shi C,Yang Y,Yu J,et al.The long noncoding RNA LINC00473,a target of microRNA 34a,promotes tumorigenesis by inhibiting ILF2 degradation in cervical cancer[J].Am J Cancer Res,2017,7(11):2157-2168.
13 张文倩.NF90通过PI3K/Akt信号通路调节宫颈癌血管生成的研究[D].重庆:重庆医科大学,2018.
14 Zhang C,Xie C,Wang X,et al.Aberrant USP11 expression regulates NF90 to promote proliferation and metastasis in hepatocellular carcinoma[J].Am J Cancer Res,2020,10(5):1416-1428.
15 Higuchi T,Todaka H,Sugiyama Y,et al.Suppression of microRNA-7(miR-7)biogenesis by nuclear factor 90-nuclear factor 45 complex(NF90-NF45)controls cell proliferation in hepatocellular carcinoma[J].J Biol Chem,2016,291(40):21074-21084.
16 Bi Y,Shen W,Min M,et al.MicroRNA-7 functions as a tumor-suppressor gene by regulating ILF2 in pancreatic carcinoma[J].Int J Mol Med,2017,39(4):900-906.
17 Fei Q,Du MY,Qian LX,et al.Feedback loop in miR-449b-3p/ADAM17/NF-κB promotes metastasis in nasopharyngeal carcinoma[J].Cancer Med,2019,8(13):6049-6063.
18 续薇,刘力华,张明威,等.Tiam1-Rac1信号转导通路及其表达量值水平与胃癌恶性表型的相关性研究[J].中国实验诊断学,2007,11(4):462-467.
19 Li N,Liu T,Li H,et al.ILF2 promotes anchorage independence through direct regulation of PTEN[J].Oncol Lett,2019,18(2):1689-1696.
20 Wen X,Liu X,Mao YP,et al.Long non-coding RNA DANCR stabilizes HIF-1α and promotes metastasis by interacting with NF90/NF45 complex in nasopharyngeal carcinoma[J].Theranostics,2018,8(20):5676-5689.
21 岑兴,廖楚航,费伟,等.HIF-1α RNAi对口腔鳞癌生长以及血管生成影响的研究[J].实用口腔医学杂志,2021,37(4):482-486.
22 Shamanna RA,Hoque M,Pe′ery T,et al.Induction of p53,p21 and apoptosis by silencing the NF90/NF45 complex in human papilloma virus-transformed cervical carcinoma cells[J].Oncogene,2013,32(43):5176-5185.
23 李越.ILF2低表达下调CENPE抑制乳腺癌细胞增殖的机制研究[D].大连:大连医科大学,2018.
24 Jiang Z,Slater CM,Zhou Y,et al.LincIN,a novel NF90-binding long non-coding RNA,is overexpressed in advanced breast tumors and involved in metastasis[J].Breast Cancer Res,2017,19(1):62.

ILF2及NF90在口腔鳞状细胞癌中的表达及临床意义

Expression and clinical significance of ILF2 and NF90 in oral squamous cell carcinoma

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 1

编辑推荐

Metrics

本文评价