lncRNA AP000487.1在食管癌中的作用及机制研究

doi:10.11904/j.issn.1002-3070.2023.02.006

实用肿瘤学杂志 ›› 2023, Vol. 37 ›› Issue (2): 123-129.doi: 10.11904/j.issn.1002-3070.2023.02.006

lncRNA AP000487.1在食管癌中的作用及机制研究

刘静¹, 褚艳杰¹, 裴凤华¹, 徐睿玲¹, 王彦博²

1.哈尔滨医科大学附属第二医院消化内科(哈尔滨 150086);
2.哈尔滨医科大学附属肿瘤医院胸外科

收稿日期:2022-11-07 修回日期:2023-02-01 发布日期:2023-05-30
通讯作者: 王彦博,E-mail:wangyanbo_034@126.com
作者简介:刘静,女,(1984-),博士,副教授,从事消化道肿瘤的基础研究。
基金资助:
黑龙江省省属高等学校基本科研业务费科研项目(编号:2022-KYYWF-0275)

Role and mechanism of lncRNA AP000487.1 in esophageal cancer

LIU Jing¹, CHU Yanjie¹, PEI Fenghua¹, XU Ruiling¹, WANG Yanbo²

1. Department of Gastroenterology and Hepatology,The Second Affifiliated Hospital of Harbin Medical University,Harbin 150086,China;
2. Department of Thoracic Surgery,Harbin Medical University Cancer Hospital

Received:2022-11-07 Revised:2023-02-01 Published:2023-05-30

摘要/Abstract

摘要： 目的探究与生存相关的lncRNA AP000487.1在食管癌发生发展中的作用及机制。方法 qRT-PCR验证与食管癌患者总生存期显著相关的lncRNA AP000696.1、LINC00689、LINC00900和AP000487.1在食管癌组织和细胞株中的表达情况;在食管癌细胞株ECA109和TE-1中过表达和沉默AP000487.1后,采用CCK-8实验检测细胞增殖、Transwell侵袭实验评估细胞侵袭、划痕实验检测细胞迁移;采用Western blot测定TLR/NF-κB信号通路相关蛋白的表达;qRT-PCR检测NF-κB活化后炎症因子的表达。结果 qRT-PCR结果显示AP000487.1在食管癌细胞株ECA109(P＜0.001)、TE-1(P＜0.001)和食管癌组织(P＜0.001)中表达显著上调;CCK-8、Transwell侵袭实验及划痕实验结果显示,在ECA109和TE-1细胞中沉默AP000487.1后降低了食管癌细胞的增殖、侵袭以及迁移能力;过表达AP000487.1后,增强癌细胞的增殖、侵袭及迁移能力;Western blot测定通路相关蛋白,结果显示沉默AP000487.1后,可抑制TLR/NF-κB信号通路,并下调p-p65和p-IκB在TE-1细胞中的表达;qRT-PCR检测NF-κB信号通路活化后炎症因子的表达,与Smart silencer-NC相比,Smart silencer-AP000487.1组TNF-α(P＜0.001)、IL-1β(P＜0.01)、IL-6(P＜0.01)表达明显下调。结论 AP000487.1在食管癌细胞株及癌组织中显著高表达,其异常表达通过调控TLR/NF-κB信号通路促进食管癌的增殖、侵袭和迁移。AP000487.1可能是食管癌的潜在治疗靶点。

关键词: 食管癌, lncRNA, AP000487.1, Toll样受体, NF-κB

Abstract: Objective To explore the role and mechanism of survival-related lncRNA AP000487.1 in the occurrence and development of esophageal cancer. Methods qRT-PCR was used to verify the expression of lncRNA AP000696.1,LINC00689,LINC00900 and AP000487.1 that significantly associated with overall survival of esophageal cancer patients in esophageal cancer tissues and cell lines.Cell proliferation was detected by CCK-8 assay,cell invasion was evaluated by Transwell invasion assay,and cell migration was detected by scratch assay.The expression of TLR/NF-κB signaling pathway related proteins was detected by Western blot and qRT-PCR. Results The expression of AP000487.1 was significantly up-regulated in ECA109 cells,TE-1 cells,and ESCA tissues(P＜0.001).Silencing AP000487.1 in ECA109 cells and TE-1 cells significantly inhibited the proliferation,invasion and migration of ESCA cells.Overexpression of AP000487.1 significantly enhanced the proliferation,invasion and migration.Western blot was used to detect pathway-related proteins,and the results showed that silencing AP000487.1 could inhibit the TLR/NF-κB signaling pathway and down-regulate the expression of p-p65 and p-IκB in TE-1 cells;Compared with the Smart silencer-NC group,the expressions of TNF-α(P＜0.001),IL-1β(P＜0.01)and IL-6(P＜0.01)in the Smart silencer-AP000487.1 group were significantly down-regulated. Conclusion AP000487.1 is highly expressed in esophageal cancer cell lines and tissues,and its abnormal expression promotes the proliferation,invasion and migration of ESCA by regulating the TLR/NF-κB signaling pathway.AP000487.1 may be a potential therapeutic target for ESCA.

Key words: Esophagus cancer, lncRNA AP000487.1, Toll-like receptor, NF-κB

中图分类号:

R735.1

刘静, 褚艳杰, 裴凤华, 徐睿玲, 王彦博. lncRNA AP000487.1在食管癌中的作用及机制研究[J]. 实用肿瘤学杂志, 2023, 37(2): 123-129.

LIU Jing, CHU Yanjie, PEI Fenghua, XU Ruiling, WANG Yanbo. Role and mechanism of lncRNA AP000487.1 in esophageal cancer[J]. Journal of Practical Oncology, 2023, 37(2): 123-129.

0
/ / 推荐

导出引用管理器 EndNote|Reference Manager|ProCite|BibTeX|RefWorks

链接本文: http://journal09.magtechjournal.com/Jwk3_syzlxzz/CN/10.11904/j.issn.1002-3070.2023.02.006

http://journal09.magtechjournal.com/Jwk3_syzlxzz/CN/Y2023/V37/I2/123

参考文献

1 Ferlay J,Colombet M,Soerjomataram I,et al.Estimating the global cancer incidence and mortality in 2018:GLOBOCAN sources and methods[J].Int J Cancer,2019,144(8):1941-1953.
2 Huang FL,Yu SJ.Esophageal cancer:Risk factors,genetic association,and treatment[J].Asian J Surg,2018,41(3):210-215.
3 杜灵彬.中国人群食管鳞癌生存分析研究新进展[J].实用肿瘤学杂志,2018,32(4):344-348.
4 Hirano H,Kato K.Systemic treatment of advanced esophageal squamous cell carcinoma:chemotherapy,molecular-targeting therapy and immunotherapy[J].Jpn J Clin Oncol,2019,49(5):412-416.
5 Lin DC,Wang MR,Koeffler HP.Genomic and epigenomic aberrations in esophageal squamous cell carcinoma and implications for patients[J].Gastroenterology,2018,154(2):374-389.
6 Lorenzi L,Avila Cobos F,Decock A,et al.Long noncoding RNA expression profiling in cancer:challenges and opportunities[J].Genes Chromosomes Cancer,2019,58(4):191-199.
7 池淑琦,汪宏波.长链非编码 RNA 与肿瘤耐药性的研究进展[J].实用肿瘤学杂志,2016,30(2):150-153.
8 Zhou L,Jiang J,Huang Z,et al.Hypoxia-induced lncRNA STEAP3-AS1 activates Wnt/β-catenin signaling to promote colorectal cancer progression by preventing m6A-mediated degradation of STEAP3 mRNA.Mol Cancer.[J].Mol Cancer,2022,21(1):168.
9 Liu J,Wang Y,Chu Y,et al.Identification of a TLR-Induced Four-lncRNA signature as a novel prognostic biomarker in esophageal carcinoma[J].Front Cell Dev Biol,2020,8:649.
10 El-Zimaity H,Di Pilato V,Novella Ringressi M,et al.Risk factors for esophageal cancer:emphasis on infectious agents[J].Ann N Y Acad Sci,2018,1434(1):319-332.
11 Yang C,Chen K.Long non-coding RNA in esophageal cancer:A review of research progress[J].Pathol Oncol Res,2022,28:1610140.
12 Liu SJ,Dang HX,Lim DA,et al.Long noncoding RNAs in cancer metastasis[J].Nat Rev Cancer,2021,21(7):446-460.
13 Zhang N,Ng AS,Cai S,et al.Novel therapeutic strategies:targeting epithelial-mesenchymal transition in colorectal cancer[J].Lancet Oncol,2021,22(8):358-368.
14 Yang J,Antin P,Berx G,et al.Guidelines and definitions for research on epithelial-mesenchymal transition[J].Nat Rev Mol Cell Biol,2020,21(6):341-352.
15 Ntoufa S,Vilia MG,Stamatopoulos K,et al.Toll-like receptors signaling:A complex network for NF-κB activation in B-cell lymphoid malignancies[J].Semin Cancer Biol,2016,39:15-25.
16 Jia D,Yang W,Li L,et al.β-Catenin and NF-κB co-activation triggered by TLR3 stimulation facilitates stem cell-like phenotypes in breast cancer[J].Cell Death Differ,2015,22(2):298-310.
17 Rajamanickam V,Yan T,Xu S,et al.Selective targeting of the TLR4 co-receptor,MD2,prevents colon cancer growth and lung metastasis[J].Int J Biol Sci,2020,16(8):1288-1302.
18 Liu R,King A,Tarlinton D,et al.The ASCIZ-DYNLL1 axis is essential for TLR4-Mediated antibody responses and NF-κB pathway activation[J].Mol Cell Biol,2021,41(12):e0025121.
19 Fakhri S,Moradi SZ,Yarmohammadi A,et al.Modulation of TLR/NF-κB/NLRP signaling by bioactive phytocompounds:A promising strategy to augment cancer chemotherapy and immunotherapy[J].Front Oncol,2022,12:834072.

[1]	王晶, 杜婧, 苏健婷, 刘庆萍, 王苹, 韦再华. 1990—2019年北京市食管癌死亡趋势年龄时期队列模型分析[J]. 实用肿瘤学杂志, 2023, 37(2): 97-101.
[2]	陆小琴, 雷娇娇, 刘雨欣, 赵华清. lncRNA XIST通过miR-375-Notch1轴调控急性T淋巴细胞白血病细胞增殖、侵袭的实验研究[J]. 实用肿瘤学杂志, 2022, 36(5): 404-410.
[3]	智亮辉, 刘伟, 李伟, 甄四虎, 焦喜林. lncRNA-ATB介导的上皮间质转化在胃癌进展中的机制研究[J]. 实用肿瘤学杂志, 2022, 36(3): 208-213.
[4]	李娜, 邓茜, 蒋建军. NF-κB信号通路在恶性肿瘤化疗耐药中的作用[J]. 实用肿瘤学杂志, 2021, 35(6): 580-584.
[5]	刘言玉, 李道娟, 梁迪, 师金, 靳晶, 单保恩, 贺宇彤. 2011—2016年河北省肿瘤登记地区食管癌发病和死亡流行情况分析[J]. 实用肿瘤学杂志, 2021, 35(4): 314-320.
[6]	刘慧敏, 乔丽颖, 钱永刚, 席云峰. 内蒙古2016年食管癌发病和死亡现状及2012—2016年趋势分析[J]. 实用肿瘤学杂志, 2021, 35(4): 351-356.
[7]	李瑞阳,方琳(综述),张艳桥(审校). 食管癌免疫治疗研究进展[J]. 实用肿瘤学杂志, 2021, 35(1): 41-45.
[8]	冯羽昕(综述),朱春荣(审校). lncRNA-HEIH在肿瘤中的研究进展[J]. 实用肿瘤学杂志, 2021, 35(1): 87-90.
[9]	李荣迪, 李学东. 非编码RNA在膀胱癌中的研究进展[J]. 实用肿瘤学杂志, 2020, 34(6): 565-570.
[10]	董满库, 姜福全, 林海冠, 孙晓敏, 刘景欣, 胡刚, 杨建武. lncRNA BLACAT1靶向LEMD1促进结直肠癌细胞的增殖和转移[J]. 实用肿瘤学杂志, 2020, 34(5): 418-424.
[11]	张遥, 范丹. 长链非编码RNA PSMA3-AS1在人胶质瘤细胞中的表达及作用研究[J]. 实用肿瘤学杂志, 2020, 34(3): 214-219.
[12]	赵楠楠, 秦文, 韩永焕, 鲁静, 李亚敏, 徐亚君. SOX11 抑制食管癌细胞增殖和迁移的机制研究[J]. 实用肿瘤学杂志, 2020, 34(1): 17-23.
[13]	赵云霞, 牛波. LncRNA NBAT1通过调控miR-3664-5p/Caspase 9分子轴对人乳腺癌细胞增殖的影响[J]. 实用肿瘤学杂志, 2020, 34(1): 24-29.
[14]	刘恒辰, 张新宇. Toll样受体信号通路作为结直肠癌潜在治疗靶点的研究进展[J]. 实用肿瘤学杂志, 2019, 33(2): 188-192.
[15]	刘仲阳, 袁欣, 周文, 李晓亮, 樊立茂. 微创小切口手术对早期胸中下段食管癌患者的治疗效果及对其疼痛的影响[J]. 实用肿瘤学杂志, 2019, 33(1): 57-61.

lncRNA AP000487.1在食管癌中的作用及机制研究

Role and mechanism of lncRNA AP000487.1 in esophageal cancer

PDF

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价