实用肿瘤学杂志 ›› 2010, Vol. 24 ›› Issue (1): 8-10.doi: 10.3969/j.issn.1002-3070.2010.01.002

• 论著 • 上一篇    下一篇

RT-PCR检测基因重组腺病毒感染AGZY表达效率及遗传稳定性研究

申宝忠1, 刘明1, 田长富2, 李任飞1   

  1. 1.哈尔滨医科大学附属第四医院(哈尔滨 150001);
    2.哈尔滨医科大学肿瘤防治研究所
  • 收稿日期:2010-01-15 出版日期:2010-02-28 发布日期:2015-01-23
  • 作者简介:申宝忠,男,(1961-),博士,主任医师,从事肿瘤影像学研究
  • 基金资助:
    黑龙江科技计划国际科技合作项目基金资助(编号WC03307)

Study of expression efficiency and genetic stability of adenovirus recombined with hIL-2 and hGM-CSF gene in AGZY Cells

SHEN Baozhong1, LIU Ming1, TIAN Changfu2, LI Renfei1   

  1. 1.The Fourth Hospital of Harbin Medical University,Harbin 150001;
    2.The Cancer Institute of Harbin Medical University
  • Received:2010-01-15 Online:2010-02-28 Published:2015-01-23

摘要: 目的 研究基因重组腺病毒转染人肺腺癌细胞AGZY后目的基因的表达效率及遗传稳定性。方法 以两种rAd分别转染AGZY细胞,RT-PCR半定量检测hIL-2和hGM-CSF基因mRNA表达。结果 rAd-hIL-2、rAd-hGM-CSF在人肺腺癌细胞AGZY内的表达量随时间的增长而增加,细胞传代对表达无明显影响。结论 rAd-hIL-2、rAd-hGM-CSF可在AGZY细胞内稳定表达外源基因,表达强度不受细胞传代影响。

Abstract: Objective To study the expressing of recombinant adenovirus with exogenesis genes in AGZY and the genetic stability.Method Transfected AGZY with two kinds of rAd.Then,detect the mRNA expression of Gene hIL-2 and Gene hGM-CSF with half-quantity RT-PCR.Result The expression level of hIL-2 and hGM-CSF in AGZY increased with time upto 72h.Conclusion The AGZY passage has no affect on the expression efficiency and the genetic stability of rAd-IL、rAd-GM-CS.

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