p21活化蛋白激酶1对喉癌细胞运动侵袭能力的影响

doi:10.3969/j.issn.1002-3070.2012.06.005

实用肿瘤学杂志 ›› 2012, Vol. 26 ›› Issue (6): 501-505.doi: 10.3969/j.issn.1002-3070.2012.06.005

p21活化蛋白激酶1对喉癌细胞运动侵袭能力的影响

何国庆¹, 管强¹, 张喜军², 张杰武¹, 姬宏飞³

1.哈尔滨医科大学附属第三医院头颈外科(哈尔滨 150081);
2.哈尔滨医科大学附属第四医院耳鼻咽喉科;
3.哈尔滨医科大学肿瘤防治研究所

收稿日期:2012-04-06 出版日期:2012-12-28 发布日期:2014-12-03
通讯作者: 张杰武,E-mail:jiewuzhang@yahoo.com.cn
作者简介:何国庆,男,(1973-),硕士,主治医师,从事头颈部肿瘤的基础和临床研究
基金资助:
黑龙江省教育厅科学技术研究面上项目(11541221)

The effect of PAK1 on migration and invasion of laryngeal cancer cells

HE Guoqing¹,GUAN Qiang¹,ZHANG Xijun²,ZHANG Jiewu¹,JI Hongfei³

1.Department of Head and Neck,The Third Affiliated Hospital of Harbin Medical University,Harbin 150081,China;
2.Department of Otolaryngology,The Fourth Hospital of Harbin Medical University;
3.The Institute of Cancer Prevention and Treatment of Harbin Medical University

Received:2012-04-06 Online:2012-12-28 Published:2014-12-03

摘要/Abstract

摘要： 目的探讨PAK1(p21 activated kinase 1)在喉癌中的表达及其对喉癌细胞运动侵袭能力的影响。方法首先获取22例喉癌和癌旁新鲜组织标本,采用Western blot方法检测PAK1的蛋白表达情况,再对63例喉癌和癌旁黏膜正常组织石蜡标本进行免疫组化研究,检测PAK1蛋白的表达,最后采用siRNA技术干扰FaDu喉癌细胞系的PAK1表达,同时进行细胞运动和侵袭能力的分析。结果 Western blot和免疫组化结果表明,PAK1在喉癌组织中的表达明显高于癌旁正常组织。喉癌细胞经PAK1 siRNA干扰后,运动和侵袭能力明显降低。结论 PAK1的激活可能参与喉癌的发生进展,下调PAK1的活性可降低喉癌细胞的运动和侵袭能力。

Abstract: Objective To investigate the expression of PAK1 in laryngeal cancer and its effect on migration and invasion of laryngeal cancer cells.Methods Firstly,22 samples of fresh laryngeal cancer tissues and adjacently normal laryngeal mucosa were collected to detect the expression of PAK1 by Western blot.Secondly,63 paraffin embedded laryngeal tissues were analyzed by immunohistochemistry to detect the expression of PAK1.Finally,We observed cell migration and invasion by knockdown of PAK1 in FaDu cell line via siRNA.Results The results from western blot and immunohistochemistry demonstrated that the expression of PAK1 in laryngeal cancer tissues was higher than that of normal mucosa.The cell migration and invasion of laryngeal squamous cell carcinoma was reduced after PAK1 was knocked down through siRNA.Conclusion The activation of PAK1 may contribute to the development and progression of laryngeal squamous cell carcinoma.Inhibition of PAK1 could decrease cell migration and invasion capability of laryngeal squamous cell carcinoma.

中图分类号:

R739.65

何国庆, 管强, 张喜军, 张杰武, 姬宏飞. p21活化蛋白激酶1对喉癌细胞运动侵袭能力的影响[J]. 实用肿瘤学杂志, 2012, 26(6): 501-505.

HE Guoqing,GUAN Qiang,ZHANG Xijun,ZHANG Jiewu,JI Hongfei. The effect of PAK1 on migration and invasion of laryngeal cancer cells[J]. PRACTICAL ONCOLOGY JOURNAL, 2012, 26(6): 501-505.

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p21活化蛋白激酶1对喉癌细胞运动侵袭能力的影响

The effect of PAK1 on migration and invasion of laryngeal cancer cells

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