miRNA-126和K-Ras及MMP-7在胃癌中的表达及其相关性分析

doi:10.11904/j.issn.1002-3070.2021.01.005

摘要/Abstract

摘要： 目的通过检测miRNA-126和预测靶基因K-Ras及MMP-7在胃癌组织中的表达情况,探索miRNA-126在胃癌发生中的作用。为miRNA-126/K-Ras、MMP-7通路研究提供新的理论依据。方法收集2015年3月—2015年12月内蒙古医科大学附属医院收治并确诊的胃癌患者的癌组织和正常组织,共50例。利用免疫组化染色技术检测K-Ras及MMP-7在胃癌组织和正常组织中的表达水平;利用real-time PCR技术检测miRNA-126在胃癌组织和正常组织中的表达水平。结果 K-Ras和MMP-7在胃癌组织中高表达;miRNA-126在胃癌组织中低表达;K-Ras和MMP-7在胃癌组织中的表达呈正相关,K-Ras及MMP-7分别表达阳性时,miRNA-126的表达量均偏低。结论K-Ras和MMP-7是胃癌的致癌因子,miRNA-126是胃癌的抑癌因子,K-Ras和MMP-7是miRNA-126的靶基因,且miRNA-126存在抑制K-Ras和MMP-7的作用,miRNA-126/K-Ras、MMP-7通路有望成为胃癌研究和治疗的新方向。

关键词: 胃癌, miRNA-126, K-Ras, MMP-7

Abstract: Objective The aims of this study were to investigate the role of miRNA-126 in the occurrence of gastric cancer cells by detecting miRNA-126 and predicting the expression of target genes K-Ras and MMP-7 in gastric cancer tissues. Which may provide a new theoretical basis for the study of miRNA-126/K-Ras and MMP-7 pathway.Methods A total of 50 gastric cancer tissues and normal tissues of gastric cancer patients who were diagnosed and accepted treatment in the Affiliated Hospital of Inner Mongolia Medical University from March,2015 to December,2015 were collected.Immunohistochemical staining was used to detect the expression of K-Ras and MMP-7 in gastric cancer tissues and normal tissues.Real-time PCR was used to detect the expression of miRNA-126 in gastric cancer tissues and normal tissues.Results K-Ras and MMP-7 were highly expressed in gastric cancer tissues.miRNA-126 was lowly expressed in gastric cancer tissues.K-Ras and MMP-7 was positively correlated in gastric cancer tissues.miRNA-126 was lowly expressed when K-Ras and MMP-7 expression was positive.Conclusion K-Ras and MMP-7 are carcinogenic factors of gastric cancer,and miRNA-126 is a tumor suppressor of gastric cancer,which is a antagonistic factor of K-Ras and MMP-7.K-ras and MMP-7 are the target genes of miRNA-126,and miRNA-126 exists to inhibit the interaction of K-Ras and MMP-7.The miRNA-126/K-Ras,MMP-7 pathway is expected to became a new direction of gastric cancer research and treatment.

Key words: Gastric cancer, miRNA-126, K-Ras, MMP-7

中图分类号:

R735.2

杜昕,曲泽,赵选忠,侯明星,王峰. miRNA-126和K-Ras及MMP-7在胃癌中的表达
及其相关性分析[J]. 实用肿瘤学杂志, 2021, 35(1): 23-27.

DU Xin,QU Ze,ZHAO Xuanzhong,HOU Mingxing,WANG Feng. Correlation analysis and expression of miRNA-126,K-Ras and MMP-7 in gastric cancer[J]. Journal of Practical Oncology, 2021, 35(1): 23-27.

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miRNA-126和K-Ras及MMP-7在胃癌中的表达
及其相关性分析

Correlation analysis and expression of miRNA-126,K-Ras and MMP-7 in gastric cancer

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